全文获取类型
收费全文 | 4783篇 |
免费 | 320篇 |
国内免费 | 448篇 |
专业分类
林业 | 447篇 |
农学 | 500篇 |
基础科学 | 403篇 |
729篇 | |
综合类 | 1590篇 |
农作物 | 278篇 |
水产渔业 | 168篇 |
畜牧兽医 | 936篇 |
园艺 | 238篇 |
植物保护 | 262篇 |
出版年
2024年 | 27篇 |
2023年 | 100篇 |
2022年 | 217篇 |
2021年 | 260篇 |
2020年 | 207篇 |
2019年 | 226篇 |
2018年 | 135篇 |
2017年 | 241篇 |
2016年 | 186篇 |
2015年 | 255篇 |
2014年 | 258篇 |
2013年 | 293篇 |
2012年 | 342篇 |
2011年 | 334篇 |
2010年 | 301篇 |
2009年 | 234篇 |
2008年 | 234篇 |
2007年 | 232篇 |
2006年 | 203篇 |
2005年 | 201篇 |
2004年 | 92篇 |
2003年 | 74篇 |
2002年 | 96篇 |
2001年 | 90篇 |
2000年 | 104篇 |
1999年 | 73篇 |
1998年 | 58篇 |
1997年 | 35篇 |
1996年 | 42篇 |
1995年 | 39篇 |
1994年 | 38篇 |
1993年 | 32篇 |
1992年 | 33篇 |
1991年 | 27篇 |
1990年 | 21篇 |
1989年 | 25篇 |
1988年 | 25篇 |
1987年 | 18篇 |
1986年 | 16篇 |
1985年 | 7篇 |
1984年 | 13篇 |
1979年 | 5篇 |
1974年 | 9篇 |
1973年 | 5篇 |
1972年 | 8篇 |
1971年 | 5篇 |
1970年 | 8篇 |
1968年 | 4篇 |
1966年 | 4篇 |
1904年 | 4篇 |
排序方式: 共有5551条查询结果,搜索用时 15 毫秒
81.
82.
83.
Xiaowen Long Renfu Wu Xugan Wu Wenjie Hou Guiping Pan Chaoshu Zeng Yongxu Cheng 《Aquaculture Nutrition》2019,25(6):1218-1230
A 120‐day trial was conducted to assess the effects of dietary fish oil replacement with vegetable oils on growth, lipid metabolism and antioxidant capacity of subadult swimming crab Portunus trituberculatus. Five isonitrogenous and isolipidic diets were formulated to replace 0, 250, 500, 750 and 1000g/kg of fish oil with a mixture of soybean and rapeseed oil (defined as D1–D5), and each treatment had 30 replicate crabs. Dietary fish oil replacement had no significant effects on growth of the crabs, while the D3 had the highest hepatosomatic index and total lipids in hepatopancreas. The triglyceride and lipase activities in hepatopancreas increased significantly with increasing dietary fish oil replacement. The D4 had the highest levels of alkaline phosphatase (ALP) and acid phosphatase (ACP) in the hepatopancreas, as well as the haemolymph ALP, ACP and peroxidase. The highest levels of haemolymph total antioxidant capacity, catalase and malondialdehyde were detected in D1. Total n‐3 polyunsaturated fatty acids levels in hepatopancreas decreased significantly with increasing dietary fish oil replacement. In conclusion, dietary fish oil replacement had no significant effects on growth of P. trituberculatus, and 500g/kg of fish oil replacement could improve antioxidant capacity, but excessive replacement level will enhance lipid accumulation and peroxidation in the hepatopancreas. 相似文献
84.
皱纹盘鲍食道的结构与功能 总被引:3,自引:0,他引:3
以组织学,组织化学,扫描电镜和透射电镜观察及酶活性测定等方法研究了皱纹盘鲍的食道,食道可分为前,中后,三段,中段又可分为食物通道和食道侧囊,食道粘膜上皮由纤毛柱状细胞,粘液细胞,闰状腺细胞,微绒毛细胞和分泌细胞组成,纤毛柱状细胞参与运输食物和分泌物,并呈现吸收细胞的结构特征;粘液细胞分泌中性和酸性粘多糖;颗粒状腺细胞内充盈分泌颗粒;微绒毛细胞呈现吸收细胞的特征;分泌细胞具有强的蛋白酶等酶活性,能以楔浆分泌形式分泌消化酶,该细胞还具有吸收和细胞内消化作用。食道中段还呈现3种植物多糖酶活性。 相似文献
85.
86.
It is hypothesized that the frequency of bacterial coldwater disease outbreaks can be reduced through the detection of the aetiologic agent, Flavobacterium psychrophilum, in broodstock followed by culling of eggs from heavily infected broodstock. Before a culling programme can be instituted, however, it is necessary to determine the sensitivity and specificity of existing assays for the detection of F. psychrophilum. In this study, tissue and ovarian fluid samples were collected from 224 fish at five hatcheries and screened using an enzyme‐linked immunosorbent assay (ELISA), a membrane‐filtration fluorescent antibody test (MF‐FAT), bacteriological culture and nested PCR. Latent class analysis was used to estimate sensitivity and specificity of kidney culture, kidney ELISA, nested PCR and MF‐FAT. Analytical sensitivity of the ELISA varied but was greatest when bacteria were cultured under iron‐limiting conditions. Diagnostic sensitivity estimates ranged from 0.02 (kidney culture) to 0.97 (kidney ELISA). Specificity estimates ranged from 0.02 (MF‐FAT) to 0.98 (kidney ELISA). In a separate challenge experiment, the ELISA confirmed the presence of F. psychrophilum in sub‐clinically infected fish. Results from this study demonstrate that the ELISA is an appropriate tool to screen broodstock and provides an indication of infection severity, which is crucial for implementation of a screening/culling programme. 相似文献
87.
Hongli Xia Huimin Fan Meng Long Jun Cheng Wenjie Chen Dapeng Yu Liqun Xia Yishan Lu 《Journal of fish diseases》2021,44(1):45-52
Streptococcus agalactiae is a Gram‐positive facultative intracellular bacterium that leads to severe economic loss of tilapia worldwide. Previous studies demonstrated that CD40 contributes to host protection against intracellular injection. In this study, CD40 was characterized from Nile tilapia (Oreochromis niloticus), named OnCD40. Sequence analysis showed that open reading frame of OnCD40 was 933 bp, containing a single peptide, a transmembrane domain and four cysteine‐rich domains. The qRT‐PCR revealed that OnCD40 was expressed in all examined tissues with the most abundant ones in spleen and thymus. After S. agalactiae stimulation, the expression of OnCD40 was significantly induced in most of the detected organs. Moreover, OnCD40‐overexpressing fish elicited significant protection against subsequent S. agalactiae challenge; approximately 10000‐fold fewer bacteria were detected in spleen of OnCD40‐overexpressing fish in comparison with control fish. Thus, CD40 had protecting function in Nile tilapia against intracellular pathogens. 相似文献
88.
Lam My Lan Jean-Claude Micha Duong Nhut Long Tran Thanh Hai 《Journal Of Applied Aquaculture》2013,25(3):168-183
ABSTRACT The on-farm trials of rotational rice-prawn farming in a semi-deep water area in the Mekong Delta of Vietnam was carried out in six rice fields (0.7–1.0 ha) to evaluate the production and economic efficiency of using low and high cost feed on prawn culture in paddies at different densities. Two stocking densities of 4 and 5 PL/m2 were investigated. Two treatments of feed types (pellets only or a combination of pellets, trash fish, and snail meat) were applied at stocking 4 PL/m2. At the treatment of 5 PL/m2, prawns were fed a combination of pellets, trash fish, and snail meat. Prawns were stocked in the mid-April and harvested in mid‐November before the next dry season rice crop. Rice farming was started 3 to 5 days after prawn harvesting. By cull harvesting during the culture period, final mean weights of prawn ranged from 47.2 to 57.2 g/prawn and the male:female ratio at harvesting was 2.5:1.0. The prawn yield of treatment 5 PL/m2 was highest (630 ± 22 kg/ha). Net profits in treatments using the combination of pellets, trash fish, and snail meat were 861 ± 193 US$/ha to 1,019 ± 25 US$/ha for the prawn crop and 1,393 ± 71 US$/ha to 1,576 ± 180 US$/ha for the whole system (prawn crop + dry rice crop) and significantly higher than in treatment using pellet only (P < 0.05). Prawns fed on pellets or a combination of pellets and snail meat both offer similar results in terms of production and economics. The dry rice crop offered high cost benefit ratio (2.29–2.33) with low operating cost (414–434 USD/ha) and it made a better use in sustainable rice fields through the rotational rice-prawn system. 相似文献
89.
90.
Junde Chen Ying Liu Ruizao Yi Long Li Ran Gao Nuohua Xu 《Journal Of Aquatic Food Product Technology》2017,26(1):86-94
Acid soluble collagen was extracted from the scales of lizardfish and characterized with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid analysis. After 8 h of collagen hydrolysis, hydrolysates had an estimated degree of hydrolysis (DH) from 4 ± 0.05 to 25 ± 0.63% (p < 0.05). All hydrolysates had angiotensin converting enzyme inhibitory and antioxidant activity. These activity levels showed little change after treatment with gastrointestinal proteases. Results indicated that the lizardfish by-products may be improved by enzymatic treatment with acid-soluble collagen from lizardfish scales. 相似文献