Pearson相关分析法在京津冀空气质量分析中的应用

通过分析北京、天津、石家庄等7个城市53个监测点的2014年4月8日至2014年7月23日连续15周6种污染指标 SO2、NO2、CO、O3、 PM10、PM2.5的监测数据,运用相关系数法,借助 SPSS软件确定了这些城市每种污染物的相关度。结果表明,北京与河北主要城市及天津在 SO2、PM10、PM2.53个主要指标上具有强相关性。最后,为京、津、冀的协同治理空气污染提出一些建议和防范措施,以期为京津冀的协同治理空气污染提供帮助。     

贮能式高压喷雾降温系统的研制

目前塑料大棚已越来越多的应用于种养行业,但大棚内部夏季温度过高的问题一直未能有效解决。喷雾降温法通过向空气中喷射细小水滴,水分子汽化吸热从而降低环境温度,是可用于不易密闭的柔性大棚的唯一降温方法。该项目组研制出了贮能式的高压喷雾降温系统,采用普通的洗车水泵作为高压水源,利用贮能管将部分动能贮存起来,在水泵停歇时通过释放压缩空气的能量维持喷雾,减少了使用成本及系统磨损,每天仅消耗1度电,已广泛用于养殖大棚的夏季降温。经过近期的不断完善,已将其用途扩展至消毒、除尘、加湿、动物免疫和夏日广场、会场、街市的降温加湿,展现出了广阔的应用前景。     

益生菌SFU—9发酵工艺的确定

益生菌具有促进动物生长，提高机体免疫能力等生理功能，常用作微生态饲料添加剂。菌体SFU－9是一株蜡状芽杆菌，该菌株在不利环境下能够以孢子形式存在，具有耐高温，耐酸碱和耐挤压等优点，较乳酸菌，酵母菌等益生菌更能保持活菌状态，适合饲料加工业的需要。该研究以菌株SFU－9进行实验，研究发酵温度，接种量，培养基初始pH值，溶解氧等因素对菌株生长的影响，确定出最佳培养条件为：培养基初始pH值为6．0－6．5，接种量5％（相对摇瓶装液量），培养温度30－32℃，装液量28-33mL/250mL。发醇液中的活菌数可达10^11cfu/mL。在此基础上，采用单因子和正交实验相结合的方法，确定出最佳发酵培养基组成为：玉米淀粉2．0％，啤酒酵母1．5％，NaH2PO40.2%,Na2HPO40.2%,玉米浆2．0％。是以最佳培养基配方给菌体提供营养，在最佳培养条件下培养，可以使发酵液中的活菌数高达10^12cfu/mL.     

Effects of ascorbic acid 2‐glucoside and alpha‐tocopherol on the characteristics of equine spermatozoa stored at 5°C

The aim of this experiment was to evaluate the effects of adding ascorbic acid 2‐glucoside (AA2G), a water‐soluble antioxidant and stable derivative of ascorbate, to the semen extender and compare it to the addition of vitamin C (Vit. C) and the fat‐soluble antioxidant α‐tocopherol (α‐Toh), both individually and in combination, on the seminal variables of equine sperm submitted to cooling for 72 h. We used two ejaculates from 10 stallions and evaluated them for motility, membrane integrity, chromatin fragmentation, mitochondrial activity and lipid peroxidation. In the analysis of lipid peroxidation, the control group showed 2506.2 ± 796.4 ng malondialdehyde/10⁸ sperm, which was higher (P < 0.05) than the groups treated with antioxidants. The average value of motility in the AA2G group was 68.4 ± 18.1%, which was higher (P < 0.05) than that observed in the control group (62.1 ± 16.2%). The variables membrane integrity, chromatin fragmentation and mitochondrial activity did not show significant difference (P > 0.05) between treatments. It was concluded that the antioxidants protected sperm cells from lipid peroxidation and that AA2G was effective during the cooling process of equine semen at 5°C for72 h, providing increased levels of total motility.     

两系杂交水稻新组合苯两优3113制种技术

根据制种实践,总结了苯两优3113的制种技术：为了获得高产、稳产,苯两优3113在制种方面应选择合适的基地,合理安排播期,合理安排父母本行比,加强肥水管理,适度割叶,严格除杂,搞好人工授粉,抓好病虫防治等。     

Bacillus spp. Inhibit Edwardsiella tarda Quorum-Sensing and Fish Infection

The disruption of pathogen communication or quorum-sensing (QS) via quorum-quenching (QQ) molecules has been proposed as a promising strategy to fight bacterial infections. Bacillus spp. have recognizable biotechnology applications, namely as probiotic health-promoting agents or as a source of natural antimicrobial molecules, including QQ molecules. This study characterized the QQ potential of 200 Bacillus spp., isolated from the gut of different aquaculture fish species, to suppress fish pathogens QS. Approximately 12% of the tested Bacillus spp. fish isolates (FI). were able to interfere with synthetic QS molecules. Ten isolates were further selected as producers of extracellular QQ-molecules and their QQ capacity was evaluated against the QS of important aquaculture bacterial pathogens, namely Aeromonas spp., Vibrio spp., Photobacterium damselae, Edwardsiela tarda, and Shigella sonnei. The results revealed that A. veronii and E. tarda produce QS molecules that are detectable by the Chr. violaceum biosensor, and which were degraded when exposed to the extracellular extracts of three FI isolates. Moreover, the same isolates, identified as B. subtilis, B. vezelensis, and B. pumilus, significantly reduced the pathogenicity of E. tarda in zebrafish larvae, increasing its survival by 50%. Taken together, these results identified three Bacillus spp. capable of extracellularly quenching aquaculture pathogen communication, and thus become a promising source of bioactive molecules for use in the biocontrol of aquaculture bacterial diseases.     

胰岛素样生长因子结合蛋白-1基因的研究进展

胰岛素样生长因子结合蛋白-1（IGFBP-1）是IGFBP～1家族的重要成员,它具有很多生物学功能。现介绍了IGFBP-1基因的定位、基因结构、生物学功能以及IGFBP-1的表达、多态及遗传效应研究,进而阐述了其在生物学研究中的重要意义及研究进展。     

蟹爪兰花粉萌发及贮藏特性（英文）

以蟹爪兰花粉为材料,采用液体培养法研究培养基组成、培养温度、花粉采集方式、贮藏温度等对花粉萌发特性的影响。结果表明:适宜蟹爪兰花粉萌发的液体培养基为:200g/L蔗糖+20 mg/L硼酸+20 mg/L二氯化钙;20℃下蟹爪兰花粉萌发时间比较短,且萌发率较高;有花丝的花粉萌发较好,花粉管生长良好。     

氮素供应形态对水稻根系形态和磷吸收的影响

 采用水培方法,研究了缺磷条件下不同形态氮（NH₄⁺、NO^₃-和NH₄NO₃）下的水稻根系形态性状,以及它们产生的后效应对磷吸收的影响。结果表明,在氮素供应充足但磷饥饿胁迫的状况下,氮素形态对根系的影响仍然十分显著。与单一的铵营养相比,铵硝混合营养增加了根长和根系的表面积、根系的密度以及磷的总吸收量;与单一的铵或硝营养相比,铵硝混合营养可增加吸收的磷从根系向地上部的运输。因此,铵硝混合营养改善低磷胁迫下水稻对磷的吸收和转运,其部分原因与氮素形态与根系形态发生有关。     

Chemical Constituents from the Flowers of Dendrobium chrysanthum

[Objective] This study aimed to investigate the chemical constituents from the flowers of Dendrobium chrysanthum and determine its bioactive compounds.[Method] The compounds were extracted by 95% alcohol and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis(1H NMR,13 CNMR and EIMS). [Result] Nine compounds were obtained and identified as stigmasterol(1), β-sitosterol(2), linoleic acid(3),stigmast-4-en-3-one(4), dibutyl phthalate(5), moscatilin(6), ergosterol(7), ergosterol peroxide(8) and daucosterol(9). [Conclusion] All compounds were isolated from its flowers for the first time.