西藏日喀则地区栽培牧草中7种微量元素的分布特征

通过对西藏日喀则地区3科43种栽培牧草中的铁、锰、钼、铜、锌、硒、钴7种微量元素的测定,探究微量元素在不同科属牧草中的分布特征。结果表明,西藏日喀则地区栽培牧草中微量元素的分布存在一定的规律性。总体而言,铁含量最高,可达800μg/g;锌次之,最高接近200μg/g;硒含量最低,含量变幅为0.002~0.013μg/g。3科牧草中,禾本科牧草铁含量相对较低,锌、铜含量相对较高;藜科牧草锌、铜含量相对较低,铁、锰含量相对较高;豆科牧草多数微量元素含量相对居中;各科牧草中的铁、锌、铜含量均属较高水平,钴均属中等水平;锰、硒、钼在3科间含量水平不一致。     

Betanodavirus ability to infect juvenile European sea bass,Dicentrarchus labrax,at different water salinity

Viral encephalopathy and retinopathy (VER) is one of the most devastating and economically relevant diseases for marine aquaculture. The presence of betanodavirus in freshwater fish is recorded, but very little is known about VER outbreaks in marine species reared in freshwater. Our study investigated the ability of betanodavirus to cause disease in European sea bass, Dicentrarchus labrax, reared at different salinity levels. Fish were challenged with RGNNV or mock infected by bath at different salinity levels (freshwater, 25‰ and 33‰). Fish were checked twice a day and the dead ones were examined by standard virological techniques, by rRT‐PCR and by histochemical and immunohistochemical analyses. All the infected groups showed a significant higher mortality rate than the one of the mock‐infected group. VERv presence was confirmed by rRT‐PCR. Histochemical and immunohistochemical analyses highlighted the typical lesions associated with VER. Our results highlight that salinity does not affect the ability of betanodavirus to induce clinical signs and mortality in European sea bass infected under experimental conditions. These results underline the great adaptation potential of VERv, which in combination with its already known high environmental resistance and broad host range, may explain the diffusion of this disease and the threat posed to aquaculture worldwide.     

Study of a germplasm collection of loquat (Eriobotrya japonica Lindl.) by multivariate analysis

Loquat species (Eriobotrya japonica Lindl.) originated in China, later was introduced in Japan. However, introduction in Europe occurred much later, in 18th century, it was introduced as an ornamental tree. Since then, the species was very well adapted to the Mediterranean climate, mainly in South East of Spain, where is located more than 50% of total European crop production. A European Project titled ‘Conservation, evaluation and collection of underutilized fruit species’ supported surveys and collection of plant material from these species in the Mediterranean basin countries. One of these surveys resulted in a germplasm collection of loquat established at IVIA, Valencia, Spain. This collection is being extended with new accessions collected across the world, currently the collection have 123 accessions under study. Germplasm management needs first a characterization of the plant material introduced and second diversity studies into the collection that could point out the variability pattern, to establish groups of accessions with similar traits and the most significant variables. These results allow organizing the information gathered for further uses. In this paper, 62 accessions from the IVIA germplasm collection were studied. Results from 43 variables were gathered and data studied by multivariate analysis. The analysis allowed summarizing the data tables with new variables containing most of the information. These results along with those obtained in the coming years will allow define a nuclear collection (minimum number of accessions that contained all the variability present in the collection).     

N-硝基三氯苯胺类化合物的杀虫活性研究

试验了6个N-硝基三氯苯胺类化合物在500mg.L-1浓度下对菜粉蝶5龄幼虫的杀虫活性。结果表明,筛选出来的化合物N-硝基-N-(N'-对乙酰基苯基)-氨基甲酰-2,4,6-三氯苯胺、N-硝基-N-(N'-间硝基苯基)-氨基甲酰-2,4,6-三氯苯胺和N-硝基-N-(N'-对硝基苯基)-氨基甲酰-2,4,6-三氯苯胺对菜粉蝶5龄幼虫均具有一定的杀虫活性,其LC50分别为447.19mg.L-1、67.63mg.L-1和224.85mg.L-1。     

Epidemiology of canine leishmaniosis in Catalonia (Spain) the example of the Priorat focus.

An epidemiological survey of canine leishmaniosis was conducted in the Priorat, a rural region in the Northeast of Spain, for 10 years (1985-1994). Seroprevalence throughout the region, determined by dot-ELISA and IFI, was 10.2% (8-12%). Forty percent of the dogs studied had a low level of anti-Leishmania antibodies, whereas only 50% were seronegative. Only one-third of the seropositive dogs had evident symptoms of the disease. Annual incidence of the disease was 5.7% and the level of endemicity was stable during the study. Four Leishmania zymodemes (MON-1, MON-29, MON-77, MON-105) were present in the focus, and their distribution in the different hosts is discussed. Apart from dogs and foxes, no other reservoir host has been found in the region.     

Effect of Sowing Date and Cutting Stage on Yield and Quality of Irrigated Barley and Triticale Used for Forage and Grain

The influence of cutting stage on forage and grain yield and quality has been studied in three barley and one triticale varieties, using two sowing dates in each of two sites. When cut at the first detectable node stage (C.31), forage yield was almost double the yield at the pseudo-stem erect stage (C.30). Barley yielded more forage than triticale at both cutting stages. Both species had similar forage protein content, but forage fibre content was greater in barley than in triticale. Cutting at stage 30 reduced grain yield by about 11 % and at stage 31 by nearly 35 %. Barley and triticale had similar grain yield. Grain protein content was not affected by forage removal, but was significantly greater in triticale than in barley. The later the stage of cutting, the greater was the delay in heading date. Triticale was more responsive to environmental conditions than barley.     

茉莉酸甲酯对杂交水稻同化物运输的调节

在杂交水稻拔节期及灌浆初期施用不同浓度(10～30mg·kg~(-1))JA-Me溶液,结果表明:JA-Me能促进杂交水稻对~(32)P和~(15)N的吸收;加速剑叶中同化物向穗部运输;同时提高剑叶的光合速率以及提高谷粒产量等。     

Epidemiology of Fasciola hepatica infection in the Paraíba River Valley, S?o Paulo, Brasil

The study revealed that although Lymnaea columella and Stenophysa marmorata were found in the same habitat, only L. columella harbored intramolluscan stages of F. hepatica. The population density of L. columella cyclically decreased between September and February, and increased from March to September. The drought that occurred between June and November 1981 dramatically reduced the number of snails collected. Larger snails were collected in March-July and November-December, while the smaller snails were collected in January-February and August-October, suggesting that there may be two generations of L. columella per year. The highest prevalence levels of infection in L. columella were: first year, June 8.82%, September 9.09% and October 10.52%; second year, March 6.25% and April 6.89%; third year, July 7.69%, August 10.25%, April 17.91% and May 13.91%. Results obtained with tracer sheep showed that the largest numbers of metacercariae were on pasture between June and October, and between March and April, but the infection was present during most of the year.     

GRASSES IN WINTER

The Aberystwyth district experienced exceptionally cold winter weather in 1954, 1955 and 1956, especially during the period from the end of January to early March. Its effect on the grasses, both in the single-plant nurseries and in the agronomic experimental areas, is briefly described.
It was observed that plants which entered the winter carrying a bulk of herbage in a winter-proud condition were at a disadvantage compared with those which had been reasonably grazed or cut back during the autumn. The younger plants were found to be better able than the older ones to withstand extreme conditions.
Suggestions are made as to how some simple-mixture swards can be managed so as to be reasonably likely to provide keep in situ during the difficult months December to April. The species under consideration are cocksfoot ( Dactylis glomerata ), timothy ( Phleum pratense and P. nodosum ), tall fescue ( Festuca arundinacea ), perennial ryegrass ( Lolium perernne ) and Italian ryegrass ( L. italicum ).