虹鳟鱼的养殖技术特点和前景展望

本文阐述了养鳟业的经济意义和价值,并从养鳟用水和生产方式、苗种生产、食用鱼养成、养鳟饲料、海水养鳟,疾病防治等方面总结了养鳟基本技术,同时对我国养鳟历史作了回顾,提出了我国养鳟业的发展前景。     

高寒鲤染色体核型分析

采用植物血球凝集素（ＰＨＡ）体内注射,肾组织细胞短期培养,常规空气干燥法制备了高寒鲤染色体,核型分析结果是：高寒鲤染色体的核型公式为２ｎ＝１００＝２８ｍ＋２８ｓｍ＋４４ｓｔｔ,染色体臂数ＮＦ＝１５６。     

渤海秋汛对虾数量预报方法的研究

渤海秋汛对虾的数量预报，是我国渔业预报中比较成功的报例之一。本文简述了此项预报工作的发展过程，介绍取得预报指标——相对资源量的原则和方法、加权系数的推算以及对各种回归方程的预报效果的检验结果，提出了针对不同资源水平的预报计算方法。此外，还对有争议的预报中的渔捞努力量因素问题进行了讨论。     

Effect of yeast polysaccharide on some hematologic parameter and gut morphology in channel catfish (Ictalurus punctatus)

A study was conducted to investigate the effect of dietary yeast polysaccharides on some hematologic parameters and intestinal morphology of channel catfish. Channel catfish were fed diets containing yeast polysaccharides at 0 (control), 0.1, 0.2, or 0.3?% for 7?weeks. Each diet was provided to 10 channel catfish specimens (5.82?±?0.13?g initial weight) replicated 3?times in individual 250?L fiberglass tanks. Some hematologic parameters, leukocyte phagocytic activity, and intestinal morphology were monitored. After 7?weeks of trial, 0.2?% yeast polysaccharides resulted in significantly higher (P?相似文献   

7种经济贝类生殖腺脂肪酸含量的研究

为了提高文蛤等经济贝类的稚贝成活率,对文蛤等7种经济贝类的生殖腺中脂肪及脂肪酸的含量进行了研究。试验结果表明:不同的贝类总脂肪含量不同,其中,缢蛏最高,可达25.48%,虾夷扇贝22.32%,文蛤21.27%,泥蚶19.89%,紫贻贝16.79%,杂色鲍15.75%,青蛤含量最低,只有14.88%。由于浙江沿海的缢蛏一般是在10~11月产卵、受精及完成胚胎发育,在这种低温条件下配子的成熟和受精卵发育需要大量的能量,缢蛏的脂肪含量为最高,精巢为11.02%(占所取样品干重),卵巢为14.46%。雌雄生殖腺的脂肪含量也不同,一般是雌性生殖腺脂肪含量明显高于雄性生殖腺。不饱和脂肪酸的含量大于饱和脂肪酸,其中,紫贻贝含量最高,为67.23%,文蛤次之67.19%,泥蚶第三64.27%,虾夷扇贝59.74%,缢蛏59.40%,青蛤最低,仅为57.51%。生殖腺的质量是繁殖成败的关键,其中脂质的积累是影响生殖腺发育的主要因素之一。     

锯缘青蟹卵巢发育的组织学观察

根据锯缘青蟹卵巢的外部形态和内部组织学特征,卵巢发育可以划分为六个时期:未发育期、发育早期、发育期、将成熟期、成熟期和排卵后期。在发育早期,多数卵母细胞胞质中有多个嗜酸性液泡,这种结构在其他十足类卵母细胞中较少见。少数个体在发育早期发生卵母细胞全面退化,这种现象可能是缺乏交配或饥饿造成的。     

几种淡水养殖鱼死后僵硬的季节变化

几种淡水养殖鱼活杀死后在室全僵的时间（Ｔｒ）同其生活时的水温（ｔ）之间存在着良好的负相关，即ｌｎＴｒ＝５．０３７－０．１１６５ｔ（ｒ＝－０．９２３６），显示出明显的季节差异。死后僵硬还受致死方法影响，表现为活杀鱼的僵硬时间长，挣扎死的短，半死的近于二者之间。据此，讨论了在温度低，僵硬期长的季节，采用冰藏或１０℃以常温条件下活杀鲜鱼流通上市，拓宽淡水养殖鱼类流通渠道的可能性及活杀鲜鱼的鲜度质量指标等     

Molecular identification and genetic analysis for 24 turf-type Cynodon cultivars by Sequence-Related Amplified Polymorphism markers

Bermudagrass (Cynodon ssp.) germplasm is genetically diverse and widely distributed in the world. The study was conducted to identify and assess the molecular variation and relationship among 24 cultivars developed in China, Australia and the USA. Sequence-Related Amplified Polymorphism (SRAP) was applied to cultivars identification in this study for the first time. Thirty of the 90 SRAP primer combinations generated a total of 274 clearly bands encompassing 249 (91%) polymorphic. Each bermudagrass cultivar has its unique binary code and can be distinguished from the others. Three distinct clusters were obtained by unweighted pair-group method with arithmetic averages based on the polymorphic markers. Coefficients of genetic distance among the genotypes ranged from 0.57 to 0.97. The results demonstrated that SRAP marker is a stable molecular marker technique for the identification of bermudagrass cultivars and their genetic relationships.     

Inhibitory effect of T-bet gene transfer on airway inflammation in a established murine allergic asthmatic model

AIM: To investigate the effect of T-bet plasmid gene transfer to airway on allergen induced airway inflammation in a murine asthmatic model. METHODS: A mouse asthma model was established by sensitization with ovalbumin (OVA). Forty C57BL/6 mice were divided into 4 groups (10 mice in each group): the normal control group (group A), the asthmatic model group (group B), the pcDNA3 plasmid group (group C), and the pcDNA3-T-bet group (group D). The animals in group B, C and D were sensitized and challenged with OVA. The animals in group A were applied with normal saline. pcDNA3 plasmid at dose of 50 μg was intranasally administered at 24 h before intranasal challenges to the mice in group C, and the 50 μg pcDNA3-T-bet plasmid for the mice in group D. Bronchial alveolar lavage fluid (BALF) was collected and lung tissues were resected at 48 h after OVA challenge for later assay. RESULTS: After administration with pcDNA3-T-bet plasmid, high level of T-bet expression at 48 h was detected in the lung tissue by Western blotting. In pcDNA3-T-bet treated asthmatic models, histological evaluation revealed the significant suppression of eosinophil peribronchial and perivascular infiltration, and reduction of epithelial damage. The numbers of eosinophils, neutrophils and lymphocytes in BALF from pcDNA3-T-bet treated mice were significantly reduced compared to those in asthmatic control group (P<0.05). The level of IL-4 in BALF was significantly decreased in pcDNA3-T-bet group compared to that in asthmatic control group (P<0.05), while the level of IFN-γ in BALF was significantly increased in pcDNA3-T-bet group. No significant change of inflammation cells and cytokines in pcDNA3 plasmid group and asthmatic control group was observed (P>0.05). CONCLUSION: Intranasal pcDNA3-T-bet plasmid transfer inhibits asthmatic airway inflammation in the murine asthmatic model, suggesting a new therapeutic strategy for allergic asthma.     

水产品养殖中如何做好渔政执法工作

河北省委省政府、省农业厅高度重视农产品质量安全问题,多次部署开展我省农业、畜牧业,水产业产品的质量安全整治行动。各地方政府、各有关部门做了大量工作,取得了可喜成绩。我省在农业部抽查的水产品中,合格率接近100％,取得认证的养殖基地、良种场都能够按要求操作,建立了生产用药和饲料记录、销售记录、生产规程等,并对产品定期检测。