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131.
Lynne AM Kariyawasam S Wannemuehler Y Johnson TJ Johnson SJ Sinha AS Lynne DK Moon HW Jordan DM Logue CM Foley SL Nolan LK 《Avian diseases》2012,56(1):192-199
Avian pathogenic Escherichia coli (APEC) cause colibacillosis, a disease which is responsible for significant losses in poultry. Control of colibacillosis is problematic due to the restricted availability of relevant antimicrobial agents and to the frequent failure of vaccines to protect against the diverse range of APEC serogroups causing disease in birds. Previously, we reported that the increased serum survival gene (iss) is strongly associated with APEC strains, but not with fecal commensal E. coli in birds, making iss and the outer membrane protein it encodes (Iss) candidate targets for colibacillosis control procedures. Preliminary studies in birds showed that their immunization with Iss fusion proteins protected against challenge with two of the more-commonly occurring APEC serogroups (O2 and O78). Here, the potential of an Iss-based vaccine was further examined by assessing its effectiveness against an additional and widely occurring APEC serogroup (O1) and its ability to evoke both a serum and mucosal antibody response in immunized birds. In addition, tissues of selected birds were subjected to histopathologic examination in an effort to better characterize the protective response afforded by immunization with this vaccine. Iss fusion proteins were administered intramuscularly to four groups of 2-wk-old broiler chickens. At 2 wk postimmunization, chickens were challenged with APEC strains of the O1, O2, or O78 serogroups. One week after challenge, chickens were euthanatized, necropsied, any lesions consistent with colibacillosis were scored, and tissues from these birds were taken aseptically. Sera were collected pre-immunization, postimmunization, and post-challenge, and antibody titers to Iss were determined by enzyme-linked immunosorbent assay (ELISA). Also, air sac washings were collected to determine the mucosal antibody response to Iss by ELISA. During the observation period following challenge, 3/12 nonimmunized chickens, 1/12 chickens immunized with 10 microg of GST-Iss, and 1/12 chickens immunized with 50 microg of GST-Iss died when challenged with the O78 strain. No other deaths occurred. Immunized chickens produced a serum and mucosal antibody response to Iss and had significantly lower lesion scores than nonimmunized chickens following challenge, regardless of the challenge strain. This study expands on our previous report of the value of Iss as an immunoprotective antigen and demonstrates that immunization with Iss can provide significant protection of chickens against challenge with three different E. coli strains. 相似文献
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The development of private rural lands can significantly fragment landscapes, with potentially negative consequences on ecosystem services. Models of land-use trends beyond the urban fringe are therefore useful for developing policy to manage these environmental effects. However, land-use change models have been primarily applied in urban environments, and it is unclear whether they can adequately predict exurban growth. This study compared the ability of two urban growth models to project exurban development in north-central Virginia and western Maryland over a 24-year period. Pattern-based urban growth models (such as SLEUTH) are widely used, but largely mimic patterns that emerge from historic conditions rather than allowing landowner decision-making to project change. In contrast, spatially-explicit econometric models (such as the complementary log?Clog hazard assessed in this study) model landowner choices as profit-maximizing behavior subject to market and regulatory constraints. We evaluated the two raster-based models by comparing model predictions to observed exurban conversion at pixel and county scales. The SLEUTH model was more successful at matching the total amount of new growth at the county scale than it was at the pixel scale, suggesting its most appropriate use in exurban areas is as a blunt instrument to forewarn potential coarse-scale losses of natural resources. The econometric model performed significantly better than SLEUTH at both scales, although it was not completely successful in fulfilling its promise of projecting changes that were sensitive to policy. The lack of significance of some policy variables may have resulted from insufficient variation in drivers over our study area or time period, but also suggests that drivers of land use change in exurban environments may differ from those identified for urban areas. 相似文献
134.
Iahtasham Khan Lothar H. Wieler Mahboob Ahmad Butt Mandy C. Elschner Ashiq Hussain Cheema Lisa D. Sprague Heinrich Neubauer 《Journal of Equine Veterinary Science》2012
Glanders is a highly infectious and zoonotic disease of solipeds caused by Burkholderia mallei. Progressive loss of efficiency and fatal outcome resulted in massive economic losses, which forced veterinary authorities throughout the world to implement disease control measures; these measures included mass testing using the complement fixation test and/or malleinization, and the culling of positives. This led to the eradication of glanders from Western Europe and North America in the 1950s. However, in the last decade, the number of outbreaks in Asia and South America increased steadily, and glanders regained the status of a re-emerging transboundary disease. Pakistan has been an endemic country for the past 120 years, but concise data on the presence of disease are not available. A total of 533 serum samples were collected from draught equines, a suspected risk group for glanders, from various districts of Punjab in Pakistan. The complement fixation test and the highly sensitive Western blot technique were used for serodiagnosis. No animal (horse, mule, and donkey) was found to be positive for infection. Glanders seems to be restricted to remote, sporadic pockets of endemicity and may cause outbreaks after being introduced into naive populations by (asymptomatic) shedders. 相似文献
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Melissa Cordero Dianne McFarlane Melanie A. Breshears Lisa M. Miller Margaret A. Miller Wendy M. Duckett 《Journal of Equine Veterinary Science》2012
The objective of this study was to determine the effect of season on the histologic and histomorphometric appearance of the normal equine pituitary gland. Pituitary glands were collected at necropsy from 121 horses throughout the year. Plasma was also collected from 59 of these horses before euthanasia. Hematoxylin and eosin stained median sagittal sections of each pituitary were evaluated and histologically graded by three pathologists. Histomorphometric analysis was performed on the same slides. Plasma α-melanocyte stimulating hormone was measured by radioimmunoassay in a subset of horses (n = 59). A total of 118 pituitary glands were included in the study after exclusions were made on the basis of the presence of pars intermedia (PI) adenomas (>5 mm). There was a positive correlation between PI hormone concentration (α-melanocyte stimulating hormone) and PI area. Pituitary gland measurements and grades from samples collected in the fall were compared with those collected in the nonfall months using t-test. The PI area, total pituitary area, and PI/total pituitary ratio were significantly greater in the fall compared with nonfall months (P < .0001, P < .01, P < .0001, respectively). Pituitary grades were also higher in the fall compared with nonfall months (P < .001). There was no seasonal difference in pars distalis or pars nervosa area. The results of this study show that the normal equine pituitary shows seasonal changes in appearance and size. These changes must be considered when using postmortem histologic evaluations in the diagnosis of pituitary pars intermedia dysfunction or for validation of antemortem diagnostic tests. 相似文献
137.
Arbour J Blais MC Carioto L Sylvestre D 《Journal of the American Animal Hospital Association》2012,48(4):256-260
Based on previous research, cats were thought to have been resistant to the development of clinical signs following infection with Leptospira spp. This case report presents three confirmed, naturally infected clinical cases of feline leptospirosis. The cases presented were all indoor/outdoor cats that were known to hunt. They were also all presented at different stages of renal insufficiency; however, they did not show any liver involvement. The authors suggest that there may be a longer incubation period in cats than dogs and recommend further research in the form of a large, clinical study. 相似文献
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