This work aimed to investigate the effect of fucoidan (FPS) on urate transporters induced by uric acid (UA). The results showed that UA stimulated the expression of glucose transporter 9 (GLUT9) and urate transporter 1 (URAT1) in HK-2 cells, and FPS could reverse the effect. Moreover, UA could activate NF-κB, JNK and PI3K/Akt pathways, but both pathway inhibitors and FPS inhibited the UA-induced activation of these three pathways. These data suggested that FPS effectively inhibited the expression induction of reabsorption transporters URAT1 and GLUT9 by UA, through repressing the activation of NF-κB, JNK and PI3K/Akt signal pathways in HK-2 cells. The in vitro research findings support the in vivo results that FPS reduces serum uric acid content in hyperuricemia mice and rats through inhibiting the expression of URAT1 and GLUT9 in renal tubular epithelial cells. This study provides a theoretical basis for the application of FPS in the treatment of hyperuricemia. 相似文献
Oak (Quercus sp.) is an excellent tree species as a windbreak, for water conservation, and for fireproofing in forests in China. However, several trees of this genus were found to be suffering from various fungal diseases. In this study, we evaluated 15 fungal pathogens that can cause dieback and canker disease in oak in China, and discovered two Cytospora species. They were identified as Cytospora quercinum sp. nov. and C. vinacea, based on detailed morphological comparisons and phylogenetic analysis of ITS, LSU, act, rpb2, tef1-α, and tub2 loci. This study is the first record of C. quercinum and C. vinacea as causal agents of dieback in oak based on pathogenicity tests conducted on 2-year-old plants in a greenhouse. In addition, this study also revealed the influence of different conditions on the growth rate of mycelia. Mycelial growth of C. quercinum and C. vinacea occurred at optimum temperatures of 20.1 and 20.8 °C, and optimum pH of 5.4 and 5.3, respectively. For these two species, utilization of glucose and fructose was highly efficient, and sucrose was the least efficient. The habitat of Quercus mongolica indicated that more attention and management are needed to prevent the occurrence of Cytospora disease in the summer in north-eastern China, and in spring and autumn in eastern and northern China. This study contributes to the understanding of the species causing canker or dieback diseases in important economic forest trees, and provides useful information for effective disease management of oak trees in China. 相似文献
Mineral-organic associations (MOAs) are the basic structural units of soil aggregates and are important reservoirs of nutrients for plants and soil microorganisms, determining the soil structure and fertility. However, the influence of exogenous dissolved organic matter (DOM) chemistry on the stability of MOAs is rarely reported.
Materials and methods
We first characterized different exogenous DOM through elemental analysis and spectroscopy analysis technologies. Then, a chamber incubation experiment was conducted with DOM addition concentration at 3 g C kg?1 red soil. Principal component analysis, redundancy analysis, and the partial least squares path model were used to better understand the effect of exogenous DOM chemistry on the stability of MOAs.
Results and discussion
The addition of DOM into the red soil significantly increased not only the organic carbon both in the bulk soil and the soil heavy fraction, but also the soil combined humus and the soil mineral-organic compound quantity. Moreover, the rice straw-derived DOM had the best effect on improving the soil mineral-organic compound quantity/degree (additional), followed by the animal-derived DOM, while the fulvic acid increased it the least. The ratios of elements (C/N ratio, O/C ratio, and H/C ratio), aromaticity (SUVA254), and phenolic C content of exogenous DOM had positively significant contributions to the stability of MOAs.
Conclusions
The rice straw-derived DOM had the greatest enhancement on the stability of the MOAs for its higher C/N ratio and phenolic groups content, so the exogenous DOM characteristics could be as an indicator in predicting the stability of the MOAs and evaluating the soil fertility.