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991.
The results of epidemiological studies of helminths in young beef cattle in Central Queensland over a 2½-year period commencing in January 1964 are reported. Studies were made on ten beef cattle properties, which have a total annual calf crop of approximately 2500 calves. Twenty-five faecal samples were collected monthly at random from 30 to 40 permanently identified calves on each property, to determine the types and intensity of helminth infections. Although rainfall was well below average, infections with most of the helminth species commonly found in Queensland were detected. Increases in infection levels occurred, after rainfall of two inches or more, in animals up to 21 months of age. Only on a property where pastures were irrigated and the stocking rate was higher than normal for the area did a high percentage of calves show clinical symptoms of parasitic gastroenteritis. Haemonchus placet and Oesophagostomum radiatum were the predominant species. An immunity developed to O. radiatum during the first 12 to 15 months of the calf's life. The immunity to H. placet was variable with some animals susceptible to re-infection up to 21 months of age. Recommendations for the control of helminths in beef cattle in this area are given. 相似文献
992.
Irrigation and tillage effects on root development,water use and yield of wheat on coarse textured soils 总被引:3,自引:0,他引:3
Summary Rapid drying of surface layers of coarse-textured soils early in the growth season increases soil strength and restricts root growth. This constraint on root growth may be countered by deep tillage and/or early irrigation. We investigated tillage and irrigation effects on root growth, water use, dry matter and grain yield of wheat on loamy sand and sandy loam soils for three years. Treatments included all combinations of two tillage systems i) conventional tillage (CT) — stirring the soil to 10 cm depth, ii) deep tillage (DT) — subsoiling with a single-tine chisel down to 35–40 cm, 40 cm apart followed by CT; and four irrigation regimes, i) I0 — no post-seeding irrigation, ii) I1 — 50 mm irrigation 30 days after seeding (DAS), iii) I2 — 50 mm irrigation 30 DAS and subsequent irrigations of 75 mm each when net evaporation from USWB class A open pan (PAN-E) since previous irrigation accumulated to 82 mm, and iv) I3 — same as in I2 but irrigation applied when PAN-E accumulated to 62 mm. The crop of wheat (Triticum aestivum L. HD 2329) was fertilized with 20kg P, 10kg K and 5kg Zn ha–1 at seeding. The rate of nitrogen fertilization was 60 kg ha–1 in the unirrigated and 120 kg ha–1 in the irrigated treatments. Tillage decreased soil strength and so did the early post-seeding irrigation. Both deep tillage and early irrigation shortened the time needed for the root system to reach a specified depth. Subsequent wetting through rain/irrigation reduced the rate of root penetration down the profile and also negated deep tillage effects on rooting depth. However, tillage/irrigation increased root length density in the rooted profile even in a wet year. Better rooting resulted in greater profile water depletion, more favourable plant water status and higher dry matter and grain yields. In a dry year, the wheat in the DT plots used 46 mm more water, remained 3.3 °C cooler at grain-fill and yielded 68% more grain than in CT when unirrigated and grown in the loamy sand. Early irrigation also increased profile water depletion, more so in CT than DT. Averaged over three years, grain yield in DT was 12 and 9% higher than in CT on loamy sand and sandy loam, respectively. Benefits of DT decreased with increase in rainfall and irrigation. Irrigation significantly increased grain yield on both soils, but the response was greatly influenced by soil type, tillage system and year. The study shows that soil related constraints on root growth may be alleviated through deep tillage and/or early irrigation. 相似文献
993.
Sex pheromone of the winter moth, a geometrid with unusually low temperature precopulatory responses
Roelofs WL Hill AS Linn CE Meinwald J Jain SC Herbert HJ Smith RF 《Science (New York, N.Y.)》1982,217(4560):657-659
The sex pheromone for the winter moth, Operophtera brumata (L.), has been identified as the novel compound (Z,Z,Z)-1,3,6,9-nonadecatetraene. The male moths respond to the pheromone at low temperatures (4 degrees to 15 degrees C) and exhibit an upper response limit that coincides with the lower response limit for other reported moth sex pheromone systems. The pheromone attracted two other geometrid species, O. bruceata (Bruce spanworm) and O. occidentalis. 相似文献
994.
J W McBride R E Corstvet D B Paulsen J R McClure F M Enright 《American journal of veterinary research》1992,53(10):1889-1894
Systemic and pulmonary antibody responses of calves to Pasteurella haemolytica were evaluated by measuring immunoglobulin production in blood for 9 days and in pulmonary lavage fluid for 7 days after intrapulmonary inoculation. Clinical signs, pulmonary lesions, pulmonary and systemic inflammatory response, and amount of antigen in lavage fluid were used to evaluate the response of calves to challenge with P haemolytica. The pulmonary response consisted of production of IgG, IgE, and IgM antibodies to P haemolytica antigens and a 17- to 68-fold increase of cells in lavage fluid 8 hours after inoculation, with a gradual decrease toward normal. Antibodies of the IgM isotype to P haemolytica were demonstrated as early as 8 hours through 7 days after inoculation in 3 of 3 calves. Of the anti-P haemolytica isotypes, IgM was found in the highest concentration. In all of the inoculated calves, IgE was found 1 to 2 days after inoculation, and IgG was found in 2 of 3 inoculated calves from day 1 through 7 after inoculation. Detection of IgG correlated with smaller pulmonary lesions. Immunoglobulin A was not detected in lavage fluid. Serum was evaluated for IgG and IgM antibody response to P haemolytica. Specific IgM was detectable 5 days after inoculation, and IgG was detectable 7 days after inoculation. Pasteurella haemolytica antigens were not detected in serum or plasma. A transient increase in neutrophil count was found 8 hours after inoculation, with return to baseline values by 24 hours after inoculation.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
995.
Aerobic bacterial populations were studied on the distal hair coat and at the skin surface of the shoulder, rump and abdomen of 10 healthy dogs. Coagulase negative staphylococci (CNS) were more frequently isolated from the hair than the skin at the shoulder and rump. There was no difference in the isolation rate of coagulase positive staphylococci (CPS) (Staphylococcus intermedius) between the hair and skin. Total skin counts were greatest on the abdomen whereas CNS counts from the hair were least at this site. There were no differences between CPS counts at the three sites on either hair or skin. The populations on the relatively unfavourable microenvironment of the distal hair may represent contamination rather than colonisation. The low populations of CPS at the skin surface also indicate contamination or transient colonisation rather than true resident status. 相似文献
996.
Spawning runs of brown trout Salmo trutta L., and rainbow trout, Oncorhynchus mykiss Walbaum, were compared in three tributaries of two lakes for timing, numbers and redd characteristics. Redd characteristics for the two species in four other streams were examined. No significant differences in redd characteristics between the two species were observed, but in all lake tributaries the possibilities of superimposition of spring spawning rainbow trout on redds of brown trout were examined. A common feature was the high ratio of numbers of spawners to the area of preferred spawning gravel, and it was concluded that in the lake tributaries, superimposition resulted in increased mortality of brown trout eggs, and in one case elimination of the brown trout population. Management possibilities for control of the species proportions in comparable situations are discussed. 相似文献
997.
Van de Walle GR Favoreel HW Nauwynck HJ Van Oostveldt P Pensaert MB 《Veterinary microbiology》2002,86(1-2):51-57
Addition of pseudorabies virus (PrV)-specific polyclonal immunoglobulins to PrV-infected monocytes induces internalization of plasma membrane anchored viral glycoproteins. This process may interfere with antibody-dependent cell lysis and resembles the well-studied physiological endocytosis process. A confocal study was designed to investigate whether the major cellular components, involved in physiological endocytosis (clathrin, actin, dynein and microtubules), play a role in this virological internalization process. In order to visualize the interaction of endosomes, which contain the internalized viral glycoproteins, with clathrin, actin, dynein and microtubules, a double labeling of viral glycoproteins and different cellular proteins was performed. Porcine monocytes were inoculated with the PrV-strain 89V87 at a multiplicity of infection of 50 for 13h. After the addition of FITC-labeled porcine polyclonal PrV-specific antibodies, cells were fixed with para-formaldehyde at different time points and afterwards permeabilized. The different cellular components were visualized with monoclonal antibodies and a Texas Red-conjugate, with the exception of actin, which was stained with phalloidin-Texas Red. The cells were analyzed by confocal microscopy. A clear co-localization was observed between the viral glycoproteins and clathrin and dynein during the internalization process. The microtubules were in close contact with the internalized vesicles. For actin no co-localization could be observed. It can be stated that clathrin, dynein and microtubules, important components during physiological endocytosis, are also of importance during the antibody-induced internalization of viral glycoproteins. 相似文献
998.
Ecotypic variations in leaf conductance, soil-to-leaf hydraulic conductance, components of tissue water potential, hydraulic architecture parameters and xylem embolism were examined in greenhouse-grown two-year-old Aleppo pine (Pinus halepensis Mill.) seedlings from six origins representing the geographic range of the species in Italy. Cortical resin composition of the seedlings was also determined. Measurements were made on well-watered seedlings and on seedlings subjected to recurring severe drought. Drought-stressed seedlings had lower mean leaf conductances, transpiration rates and soil-to-leaf hydraulic conductances than well-watered seedlings. They also exhibited more negative osmotic potentials, higher relative water deficit at incipient plasmolysis, but a similar maximum modulus of elasticity. Drought-stressed seedlings showed a higher degree of xylem embolism, a lower Huber value, lower leaf specific conductivity and lower specific conductivity than well-watered seedlings. Drought-stressed seedlings of provenances from more xeric habitats (Tremiti, Porto Pino and Mottola) had greater leaf conductances, transpiration rates and soil-to-leaf hydraulic conductances than drought-stressed seedlings of provenances from more mesic habitats (Imperia, Otricoli and Vico del Gargano). They also showed higher osmotic adjustment and a lower degree of xylem embolism. Among provenances, there were no significant differences in hydraulic architecture parameters in response to the drought treatment; however, Tremiti and Porto Pino seedlings displayed smaller drought-induced reductions in specific conductivity and leaf specific conductivity, respectively, than seedlings from other provenances. These differences suggest that seedlings from xeric provenances, especially Tremiti, have greater resistance to desiccation than seedlings from mesic provenances. No clear association was found between terpene variability and the other traits investigated, although terpene composition was related to the geographical distribution of the provenances. We conclude that the drought-tolerance responses of Tremiti make it a more suitable provenance than the others for establishment on sites prone to severe soil water deficits. 相似文献
999.
Freeman RG Grabar KC Allison KJ Bright RM Davis JA Guthrie AP Hommer MB Jackson MA Smith PC Walter DG Natan MJ 《Science (New York, N.Y.)》1995,267(5204):1629-1632
The self-assembly of monodisperse gold and silver colloid particles into monolayers on polymer-coated substrates yields macroscopic surfaces that are highly active for surface-enhanced Raman scattering (SERS). Particles are bound to the substrate through multiple bonds between the colloidal metal and functional groups on the polymer such as cyanide (CN), amine (NH(2)), and thiol (SH). Surface evolution, which can be followed in real time by ultraviolet-visible spectroscopy and SERS, can be controlled to yield high reproducibility on both the nanometer and the centimeter scales. On conducting substrates, colloid monolayers are electrochemically addressable and behave like a collection of closely spaced microelectrodes. These favorable properties and the ease of monolayer construction suggest a widespread use for metal colloid-based substrates. 相似文献
1000.
Agreement of high definition oscillometry with direct arterial blood pressure measurement at different blood pressure ranges in horses under general anaesthesia 下载免费PDF全文
ObjectiveTo determine the agreement of high definition oscillometry (HDO) with direct arterial blood pressure measurements in normotensive, hypotensive and hypertensive horses during general anaesthesia.Study designExperimental study.AnimalsSeven healthy warmblood horses, aged 3–11 years, weighing 470–565 kg.MethodsMeasurements from a HDO device with the cuff placed around the base of the tail were compared with pressures measured invasively from the facial artery. High blood pressures were induced by intravenous (IV) administration of dobutamine (5 μg kg−1 minute−1) over ten minutes followed by norepinephrine (0.1 mg kg−1 IV) and low pressures by increasing the inspired fraction of isoflurane and administration of nitroglycerine (0.05 mg kg−1 IV). For analysis three pressure levels were determined: high (MAP>110 mmHg), normal (60 mmHg