Epidemiological study of enzootic pneumonia in dairy calves in Saskatchewan.

A field study involving 325 calves from 17 dairy herds in Saskatchewan was conducted to determine the risk of enzootic pneumonia and to assess its association with a number of factors. Two different case definitions of pneumonia were used in the analyses: the first was based on producers' treatment risk (CASE1) and the second was based on semimonthly clinical examinations of calves by the research veterinarian (CASE2). The risk of pneumonia based on CASE1 was 39% and on CASE2 was 29%. The measure of agreement between CASE1 and CASE2 at the calf level of analysis was poor (kappa = 0.24, SE = 0.02) and at the herd level of analysis was moderate (kappa = 0.40, SE = 0.12). The mortality risk from pneumonia was 1.8% and a variety of infectious organisms were isolated from pneumonic lungs. Twenty-seven percent of the calves had inadequate (total IgG < or = 800 mg/dL) levels of passively acquired antibodies as measured by radial immunodiffusion. The proportion of seropositive titers in calves within the first two weeks of age was 94% to parainfluenza 3 virus (PI3V) and bovine respiratory syncytial virus (BRSV), 73% to Pasteurella haemolytica (Ph), 68% to bovine viral diarrhea virus (BVDV), 67% to infectious bovine rhinotracheitis virus (IBRV), 46% to Mycoplasma dispar (Md), 44% to Haemophilus somnus (Hs), and 21% to Mycoplasma bovis (Mb). At the calf level of analysis and after adjusting for clustering, there was a negative association (p = 0.10) between the diagnosis of pneumonia based on CASE2 and total IgG levels and Ph titers (rPh).(ABSTRACT TRUNCATED AT 250 WORDS)     

不同种类杀虫剂对斜纹夜蛾的药效评价

通过室内测定和田间药效试验，评价了有机磷、拟除虫菊酯、氨基甲酸酯、昆虫生长调节剂、苯基吡唑类、抗生素及植物性农药等７大类杀虫剂对斜纹夜蛾的效应，结果表明，毒死蜱、保得、万灵及抑太保对斜纹夜蛾具有优良的防效，可作为首选杀虫剂进行交替使用     

奶牛冷冻胚胎规模化移植效果研究

为了提高奶牛良种化程度,对奶牛冷冻胚胎的规模化移植效果进行了研究。结果表明:PG二次肌注法的发情率显著高于(P<0.05)PG一次肌注法,两种方法的发情同期率均超过85%,受体牛发情时间多集中在肌注PG后48～72h;胚胎解冻后的复苏率达到82.58%,且早期囊胚与囊胚的复苏率要高于桑椹胚(P>0.05);共移植受体牛218头,受胎97头,受胎率为44.5%,且春、秋季的移植效果差异不显著(P>0.05)。为奶牛冻胚的规模化移植研究提供了参考依据。     

微波电路电测及其计算方法

从实际工程的角度出发，对电路电测方法进行了论述。在微通信工程中，需要获得障碍物的位置和高度，最合理的天线高度组合和障碍物绕射损耗等参数，为此论述了微波电路电测方法及技术要求。在大庆－秦皇岛输油管道通信工程中采用了Ａ，Ｂ测试法进行测试，得到高度－损耗曲线。这种曲线给出相对自由空间接收电平与收，发天线高度之间的关系。在测试所得的高度－损耗曲线与断面图相结合的基础上，由绕射损耗计算公式推出了几种常见障碍     

马尾松不同苗木类型与整地方式造林早期效果的研究

通过对不同苗木类型和整地方式的马尾松幼林的造林成活率、生长状况、生物量和根系特征进行测定，结果表明：容器苗可明显提高造林成活率、当年抽梢高和生物量；整地方式对造林当年的马尾松幼林有一定影响，而在第３年则对幼林树高和抽梢高起着明显促进作用。     

甲状腺球蛋白免疫组化对甲状腺肿瘤的诊断价值

用免疫组化A B C法对60例甲状腺病理标本进行了甲状腺球蛋白检查,结果表明源于甲状腺滤泡上皮细胞的肿瘤,均呈阳性或弱阳性反应。大部分甲状腺癌呈弱阳性反应。作者认为甲状腺球蛋白可作为鉴别甲状腺滤泡上皮细胞源性与非滤泡上皮细胞源性的良好标记。     

Alkaline phosphatase isoenzymes in feline serum using an agarose gel alkaline phosphatase kit method.

Total serum alkaline phosphatase (ALP) activity is the product of the combined activity of isoenzymes from a number of tissue sources. In this study, a commercially available kit for electrophoretic separation of ALP isoenzymes in an agarose gel was used to separate ALP isoenzymes in feline tissue extracts and serum. Five separate bands of ALP activity were identified. These bands were numbered 1 to 5 with band 1 having the most anodal migration. The tissue of origin corresponding to the migration position of the isoenzymes are as follows: Band 3 was the liver isoenzyme, band 4 was the bone isoenzyme and ALP isoenzymes of both intestine and kidney migrate in the position labelled band 5. Band 1 appears to be related to albumin and does not represent true ALP activity. The tissue source of band 2 (a and b) was not identified. Serum ALP activity of mature, healthy cats is primarily of liver origin. Immature cats (< 1 year of age) have a greater proportion of the bone isoenzyme in the serum.     

明确目标 加快发展全面推进江苏水利现代化进程 明确目标 加快发展全面推进江苏水利现代化进程 明确目标 加快发展全面推进江苏水利现代化进程

全面推进现代化建设，是21世纪的三大任务之一。水利作为首位国民经济基础设施，在经济和社会的发展过程中有着不可替代的战略地位。全面加快现代化建设，水利现代化必须先行，并适当超前。 1　全面推进水利现代化建设是我省经济社会和水利事业发展的客观要求 　　江苏滨江临海，地处江淮沂沭泗的下游，河湖众多，地势低洼，全省10多万km2的面积承泄上中游200多万km2的来水，汛期80%的地面处于洪水威胁之下，素有“洪水走廊”之称。江苏又处于南北气候过渡地带，降水时空分布不均，水资源也相对紧缺，淮北地区、沿海垦区、丘陵山区水资源不足的矛盾尤为突出，苏南面临着水质型缺水的问题。特殊的地理位置和气候条件决定了江苏是一个水旱灾害频发的省份。据统计，1949年后的50年中，江苏受灾面积在66.7万hm2以上的水旱灾害共44年，其中洪涝15年、干旱10年、水旱交错19年，正常年景仅6年。因此，水利在江苏经济社会发展中始终处于十分重要的位置。江苏近50年经济与社会发展的历史证明，“经济要发展，水利必先行。”刚刚结束的江苏省委九届十二次全会明确提出：“十五”期间要以富民强省、争取率先基本实现现代化来总揽全局。在这种形势下，加快水利现代化建设进程，既是全省现代化建设的要求，也是水利自身发展的要求。 1.1　全面推进水利现代化是江苏社会和经济发展的必然需求 　　江苏经济较为发达，　1999年GDP达到7 700亿元，　居全国第二位，　占全国总量的9.4%；　人均GDP达10 669元，　已接近中等收入国家下限水平。　《中共江苏省委关于制订江苏省国民经济和社会发展第十个五年计划的建议》提出：　“十五”　是江苏全面建设宽裕的小康社会，　为率先基本实现现代化奠定坚实基础的关键时期，　全省GDP年均增长预期调控目标为9%～10%，　人均GDP年均增长8%～9%。　到2010年，　全省GDP在2000年的基础上增长1.4倍左右，　苏南及其他有条件的地方基本实现现代化。　水利作为国民经济重要的基础设施，　必须保持与经济和社会的同步发展，　并适度超前。　随着全省现代化进程的加快，　必然要求加快水利现代化建设步伐，　进一步提高防洪保安能力、　水资源供给水平和水环境保护能力，　为国民经济和社会发展提供更为可靠的水利保障。 1.2　全面推进水利现代化是江苏水利发展到新阶段的客观要求 　　1949年以来，　在历届省委、省政府的领导下，江苏人民 　　　本文为江苏省水利厅黄莉新厅长2000年12月15日在部分省、市农村水利现代化研讨会上的发言，略有删节。     

近交系大鼠DNA指纹图与微卫星DNA比较分析

为了建立一种对近交系大鼠遗传物质进行精确可靠、快速简便的监测方法,利用DNA指纹技术对国内6个品系8个近交系大鼠群体进行了DNA多态性的分析,并与PCR扩增微卫星DNA技术进行了比较.其结果显示(1)不同品系之间DNA指纹图差异较大,同一群体不同个体间DNA指纹图带的相似系数和共有带率除SHR(哈)和WKY(哈)小于0.7外,其他均大于0.9.不同地区同一SHR间和WKY间DNA指纹图也存在差异,相同DNA不同次制作的DNA指纹图谱基本一致.(2)不同品系个体间微卫星DNA具有显著多态性;同一群体不同个体之间除SHR(哈)的SMST位点和WKY(哈)的AGT位点出现一定的差异外,其他均没有差异.DNA指纹图能更精确可靠地反映出动物个体间的遗传背景,而微卫星DNA遗传监测方法比较简便快捷.     

Nephrotoxicity of amphotericin B in dogs: a comparison of two methods of administration.

Two methods of administration of amphotericin B were compared for their ability to produce nephrotoxicity in 12 dogs. Six dogs received six alternate day doses of amphotericin B: 1 mg/kg administered as a rapid bolus in 25 mL 5% dextrose in water. Another six dogs received alternate day treatments of the same dose of amphotericin B in 1 L 5% dextrose in water over 5 h. Both treatment groups experienced significant reductions in glomerular filtration rate, as measured by inulin clearance, 24 h endogenous creatinine clearance, serum creatinine and serum urea. This reduction in glomerular filtration rate was most marked in the group receiving the drug as a rapid bolus. The inulin clearances decreased from 3.54 +/- 0.30 mL/min/kg (means +/- SEM) on day 0 to 1.15 +/- 0.25 mL/min/kg on day 12 in the slow infusion group and from 3.24 +/- 0.25 mL/min/kg on day 0 to 0.46 +/- 0.11 mL/min/kg on day 12 in the rapid bolus group. Renal lesions characteristic of amphotericin B administration were observed in all dogs tested. The dogs which received amphotericin B as a rapid bolus had a significantly greater number of tubular lesions than the slow infusion group. Systemic side effects, such as vomiting, diarrhea and weight loss, were observed in both treatment groups but were most severe in the rapid bolus group.(ABSTRACT TRUNCATED AT 250 WORDS)