紫花苜蓿不同基肥配比效应的研究

针对云南紫花苜蓿Medicago sativa种植缺乏专用肥的研究,采用"3414"的试验设计来研究不同基肥配比对紫花苜蓿第1年产量、株高、叶茎比、单株质量的影响.试验得出,施N 57.3 kg/hm2、P2O5 138 kg/hm2、K2O 36.45 kg/hm2时年产最高,达104 865 kg/hm2;施N 129.3 kg/hm2、P2O5 -5.1 kg/hm2(视为不施磷肥)、K2O 82.5 kg/hm2时茎叶比最大,达52.12%;施N 79.35 kg/hm2、P2O5 73.05 kg/hm2、K2O 106.05 kg/hm2时株高最大,达61.56 cm;施N 24.9 kg/hm2、P2O5 79.05 kg/hm2、K2O 80.1 kg/hm2时单株质量最高,达5.58 g.     

The Productive Performance and the Correlation Analysis Between Hatchability and Egg Quality in Jiangxi Green-eggshell Layers

The purpose of this study was to provide the reference to Jiangxi Green-eggshell breeding through the analysis of laying performance, hatchability and egg quality in the first generation of Jiangxi Green-eggshell layers at 300-days old. 306 Jiangxi Green-eggshell layers and 80 eggs were selected to determine the laying performance, hatchability and egg quality among families, and the correlation between the hatchability and egg quality were analyzed also. The results were as fellows:The average body weight of these hens was 1 596.99 g, the average egg weight was 50.57 g, the average egg-laying number was 14.53, the average fertility rate was 93.69%, the average dead fertile egg rate was 5.55%, the average healthy chick rate was 84.3%, the average dead embryo egg rate was 13.9%, the average egg shape index was 1.28, the average Haugh unit was 71.77, and the average eggshell strength was 4.44 kg/cm². The healthy chick rate and the egg weight had significant correlation,as well as the eggshell color and the egg shape index (P < 0.05). The healthy chick rate had extremely significantly correlation with the dead fertile egg rate(P < 0.01), as well as the eggshell strength and the eggshell thickness (P < 0.01). Generally, the first generation of Jiangxi Green-eggshell layers had good egg quality, higher laying performance and hatchability, but they still need to be further selected.     

Chemical composition and microstructure of uroliths and urinary sediment crystals associated with the feeding of high-level cottonseed meal diet to water buffalo calves

The chemical composition and microstructure of seven uroliths and four urinary sediment samples associated with the feeding of high-level cottonseed meal diet to buffalo calves were examined by chemical qualitative analyses, scanning electron microscopy ( ), X-ray diffraction, and X-ray energy dispersive spectrometry ( ). Struvite was a major component of kidney stones and of some bladder stones. The kidney stone sample appeared cracked under low power under , aggregated into tiny balls under high power, and as a bladelike structure under even higher power. The bladder stone samples appeared finely granular or granular with various forms of prismatic crystals. The urinary sediments were prismatic crystals, with granules. The newly found prismatic crystals, which were rich in potassium and similar to struvite in crystal structure, were identified as potassium magnesium phosphate (KMgPO₄ · 6H₂O) in some bladder stones and urinary sediments. However, crystals which contained Mg and P only, which had been used for struvite identification, were not found by examination in urinary sediments from fresh urine samples of buffalo calves fed the high-level cottonseed meal diet.     

猪毛滴虫病病原及诊治的研究进展

猪毛滴虫病的历史较短,关于该病的研究和病例报道也较少。近年来,随着该病导致保育猪腹泻病例日益增多,猪毛滴虫病对养猪业的危害日益显现。本文就该病的病原、危害性、致病机理、流行病学、临床症状、病理变化、诊断以及防治作一综述,旨在为猪毛滴虫病的研究和防控提供参考。     

人参三醇组皂苷对大鼠急性坐骨神经损伤修复相关蛋白表达的影响

研究人参三醇组皂苷(PTS)对大鼠坐骨神经急性损伤蛋白表达的影响。建立大鼠坐骨神经挤压损伤模型,随机分为PTS组,模型对照组及空白对照组,每组各10只大鼠;各组均经腹腔注射给药,PTS组损伤后注射PTS;模型对照组,损伤后注射同剂量生理盐水;空白对照组,不损伤坐骨神经,注射同剂量生理盐水。坐骨神经损伤术后30d,免疫荧光化学染色检测PTS对损伤侧坐骨神经神经丝蛋白(NF-M)、微管相关蛋白-2(MAP-2)、生长相关蛋白(GAP-43)表达的影响,观察PTS对损伤侧神经的影响。大鼠损伤侧坐骨神经NF-M、MAP-2、GAP-43平均光密度表达(9.66±0.99、12.43±1.44、0.344±0.040)与模型对照组(8.21±1.25、11.90±1.14、0.278±0.042)比较有极显著性差异(P0.01)。PTS可促进坐骨神经损伤大鼠NF-M,MAP-2,GAP-43的表达。     

基于TM和ASTER的植被指数建模性能对比

在对TM、ASTER数据进行多种植被指数生成的基础上,结合研究区48个杨树样地的测树因子数据,分别就杨树单位面积蓄积量、杨树平均高和杨树年龄进行了遥感统计建模研究,通过植被指数及其变形式共65个特征对比结果表明,由ASTER数据派生的植被指数中RVI3(0.5)对于上述3个测树因子的估测性能最好,由TM数据导出的植被指数中则是DVI2对上述3个测树因子回归效果最好,从建模性能上分析,ASTER数据生成的植被指数要优于TM数据生成的植被指数。     

Real-time PCR和常规PCR方法快速鉴定肉骨粉中的牛源性成份

疯牛病是一种严重危害动物和人类健康的疾病,其病原朊病毒可以通过食物链进行传播,因此建立准确的检测牛源性成份的方法非常重要.我们采用常规PCR和Real-time PCR中的TaqMan技术建立了快速鉴定牛源性成份的方法,使用Chelex-100提取肉骨粉中的DNA,过程简单可靠仅需要20min左右.对不同含量的牛肉骨粉进行检测,常规PCR能够检测到的最低含量是0.001%,而Real-time PCR对相同的样品进行检测所得到的灵敏度更高,最高可达到0.0001%,两种PCR方法的鉴定过程都非常简单快速,各需要2 h左右.这两种方法可以根据各实验室的不同条件作为肉骨粉鉴别的常规检测方法.     

老年犬的养护

如果家里有一头老年犬,它一定陪伴你走过了好几个年头,在这么多年里它也许是你的精神寄托;有了它你的生活更快乐了,是它提高了你的生活品质。这个时候,它仍然在你的身旁,但它已经不像年轻时那样爱玩爱闹了,对事情的注意力、警惕性也有明显下降。这些都是不可避免的,你深爱的犬正在逐渐衰老。这个时候,它的需求正在变化,它对你的依赖越来越强,它需要你给它保持一个健康的身体和安逸的老年生沽。     

家禽脂肪营养研究进展

脂肪代谢能值高,且是必需脂肪酸的来源,在家禽营养中发挥着重要的作用。饲料中添加合适的脂肪能改善家禽的生产性能、屠体品质、免疫性能、产蛋性能、蛋品质及繁殖性能。本文综述了国内外近十年关于脂肪在肉禽、蛋禽和种禽上的研究报道,旨在为脂肪在家禽饲料中的合理应用提供科学依据。