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41.
A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
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杨桃果实生长发育过程中营养品质的变化 总被引:8,自引:0,他引:8
杨桃(Averrhoa arambola L.)为华南地区重要的热带水果。本试材为华南农业大学园艺分场种植的3年生盆栽马来西亚甜杨桃,砧木为酸杨桃。在杨桃的开花期,选择生长势、开花数量和时间较一致45株为试材,再选择受精时间一致的果实做标记。采样时问从开花后(2003年7月15日)至杨桃充分成熟(2003年10月25)。测定杨桃果实中的糖(蒽酮比色法)、有机酸(HPLC法)、维生素C(2,6-二氯靛酚钠滴定法)和单宁(高锰酸钾滴定法)的含量。 相似文献
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《动物防疫法》自1998年1月1日实施至今已有7年了,其在加强动物防疫工作的管理及预防、控制和扑灭动物疫病,促进养殖业发展,保护人体健康方面发挥了积极的作用。但在实际工作中,我们常常感到《动物防疫法》中的一些条款规定不明确、不具体,造成可操作性差,难以落实;同时一些罚则设置不当,处罚力度小,与相关法律衔接不上,起不到应有的作用。 相似文献
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AIM:To explore the effect of brain ischemia injury on cell proliferation and nestin expression in cortex and subependymal zone (SEZ).METHODS:Using a local brain ischemia model(MCAO), BrdU positive cells of cortex and subependymal zone (SEZ), also nestin positive cells, were observed by immunohistochemistry.RESULTS:BrdU and nestin positive cells in SEZ of MCAO rats were obviously increased. In cortex, only nestin positive cells were observed.CONCLUSION:Neural stem cells in SEZ and cortex were activated after brain ischemia, it may be related with neural recovery after brain ischemia injury. 相似文献
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Glucoraphanin (4-甲基亚磺酰丁基芥子油苷 )在青花菜中含量丰富 ,其降解产物Sulforaphane具很强的抗癌活性。本试验以‘Marathon’青花菜为试材 ,将新鲜花球切成小花球后用 10 0mg/L的NaClO表面消毒 1min ,清水洗净。分别取 6 5 0g花球置于 0℃、 5℃和 10℃冷藏 ,并在 5℃下进行 5种气调处理 :1)Air,2 )Air+10 %CO2 ,3)Air+2 0 %CO2 ,4) 1%O2 ,5 ) 1%O2 +10 %CO2 。每一处理分别在处理当天、处理后 3、 6、 9、 12d取样 5 0g ,真空冷冻干燥 ,并研磨成粉末状贮藏于 4℃待用。将 0 .2g冷冻干燥样品在沸水中煮 10min ,35 0 0g离心 5m… 相似文献
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Shohei MATSUURA Shigeru HOSHINO Hideaki HAYASHI Tetsuyuki KOHGUCHI Kyoji HAGIWARA Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102
DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum
production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently
infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV
originating from latently infected stock plants in chrysanthemum production fields.
Received 27 July 2001/ Accepted in revised form 27 November 2001 相似文献
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