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11.
Long unfolded linkers facilitate membrane protein import through the nuclear pore complex 总被引:1,自引:0,他引:1
Meinema AC Laba JK Hapsari RA Otten R Mulder FA Kralt A van den Bogaart G Lusk CP Poolman B Veenhoff LM 《Science (New York, N.Y.)》2011,333(6038):90-93
Active nuclear import of soluble cargo involves transport factors that shuttle cargo through the nuclear pore complex (NPC) by binding to phenylalanine-glycine (FG) domains. How nuclear membrane proteins cross through the NPC to reach the inner membrane is presently unclear. We found that at least a 120-residue-long intrinsically disordered linker was required for the import of membrane proteins carrying a nuclear localization signal for the transport factor karyopherin-α. We propose an import mechanism for membrane proteins in which an unfolded linker slices through the NPC scaffold to enable binding between the transport factor and the FG domains in the center of the NPC. 相似文献
12.
Liesbeth Vogel Mariken Van der Lubben Eddie G. Te Lintelo Cornelis P.J. Bekker Tamara Geerts Leontine S. Schuijff Guy C.M. Grinwis Herman F. Egberink Peter J.M. Rottier 《Veterinary research》2010,41(5)
Feline coronaviruses (FCoV) comprise two biotypes: feline enteric coronaviruses (FECV) and feline infectious peritonitis viruses (FIPV). FECV is associated with asymptomatic persistent enteric infections, while FIPV causes feline infectious peritonitis (FIP), a usually fatal systemic disease in domestic cats and some wild Felidae. FIPV arises from FECV by mutation. FCoV also occur in two serotypes, I and II, of which the serotype I viruses are by far the most prevalent in the field. Yet, most of our knowledge about FCoV infections relates to serotype II viruses, particularly about the FIPV, mainly because type I viruses grow poorly in cell culture. Hence, the aim of the present work was the detailed study of the epidemiologically most relevant viruses, the avirulent serotype I viruses. Kittens were inoculated oronasally with different doses of two independent FECV field strains, UCD and RM. Persistent infection could be reproducibly established. The patterns of clinical symptoms, faecal virus shedding and seroconversion were monitored for up to 10 weeks revealing subtle but reproducible differences between the two viruses. Faecal virus, i.e. genomic RNA, was detected during persistent FECV infection only in the large intestine, downstream of the appendix, and could occasionally be observed also in the blood. The implications of our results, particularly our insights into the persistently infected state, are discussed. 相似文献
13.
Dana Nigussie van der Waaij Liesbeth H. Dessie Tadelle van Arendonk Johan A. M. 《Tropical animal health and production》2010,42(7):1519-1529
To generate information essential for the implementation of breeding schemes suitable for village poultry producers in Ethiopia,
a survey was conducted aimed at defining the socioeconomic characteristics of the production environments in different geographic
regions, understanding the important functions of chickens, identifying farmers’ choice of chicken breeds and the underlying
factors that determine the choice of genetic stock used. The survey included both questionnaire survey and a participatory
group discussion. A total of 225 households (45 households from each of five Woredas) were interviewed. The questionnaire was designed to collect data covering general information on village poultry production
such as socio-management characteristics, production objectives, population structure, breed choice and trait preferences,
market preferences of specific traits, and farmers’ selection practices. The participatory farmers’ discussions were designed
to involve stakeholders in defining the breeding objective “traits” and deriving their relative importance in the production
environment based on the different functions of chickens and “traits” identified in the interviews. The results showed that
production of eggs for consumption is the principal function of chickens in most regions followed by the use as source of
income and meat for home consumption. The production system in all geographic regions studied revealed similar features generally
characterized by extensive scavenging management, absence of immunization programs, increased risk of exposure of birds to
disease and predators, and reproduction entirely based on uncontrolled natural mating and hatching of eggs using broody hens.
Farmers’ ratings of indigenous chickens with respect to modern breeds showed the highest significance of the adaptive traits
in general, and the superior merits of indigenous chickens to high yielding exotic breeds in particular. Adaptation to the
production environment was the most important attribute of chickens in all the study areas. The high significance attributed
to reproduction traits indicates the need for maintaining broody behavior and high level of hatchability while breeding for
improved productivity of indigenous chickens for village conditions. The market price of chickens is primarily dictated by
weight, but farmers rated growth (males) and number of eggs followed by growth (females) as the production traits they would
like the most to be improved. Therefore, the ultimate breeding goal should be to develop a dual-purpose breed based on indigenous
chicken genetic resources with any of the comb types other than single for all the regions studied having the most preferred
white body plumage for farmers in the Amhara region and red body plumage for those in Oromia, Benshangul-Gumuz, and Southern
regions. 相似文献
14.
Daniel Pauly Dyhia Belhabib Roland Blomeyer William W W L Cheung Andrés M Cisneros‐Montemayor Duncan Copeland Sarah Harper Vicky W Y Lam Yining Mai Frédéric Le Manach Henrik Österblom Ka Man Mok Liesbeth van der Meer Antonio Sanz Soohyun Shon U Rashid Sumaila Wilf Swartz Reg Watson Yunlei Zhai Dirk Zeller 《Fish and Fisheries》2014,15(3):474-488
We conservatively estimate the distant‐water fleet catch of the People's Republic of China for 2000–2011, using a newly assembled database of reported occurrence of Chinese fishing vessels in various parts of the world and information on the annual catch by vessel type. Given the unreliability of official statistics, uncertainty of results was estimated through a regionally stratified Monte Carlo approach, which documents the presence and number of Chinese vessels in Exclusive Economic Zones and then multiplies these by the expected annual catch per vessel. We find that China, which over‐reports its domestic catch, substantially under‐reports the catch of its distant‐water fleets. This catch, estimated at 4.6 million t year?1 (95% central distribution, 3.4–6.1 million t year?1) from 2000 to 2011 (compared with an average of 368 000 t·year?1 reported by China to FAO), corresponds to an ex‐vessel landed value of 8.93 billion € year?1 (95% central distribution, 6.3–12.3 billion). Chinese distant‐water fleets extract the largest catch in African waters (3.1 million t year?1, 95% central distribution, 2.0–4.4 million t), followed by Asia (1.0 million t year?1, 0.56–1.5 million t), Oceania (198 000 t year?1, 144 000–262 000 t), Central and South America (182 000 t year?1, 94 000–299 000 t) and Antarctica (48 000 t year?1, 8 000–129 000 t). The uncertainty of these estimates is relatively high, but several sources of inaccuracy could not be fully resolved given the constraints inherent in the underlying data and method, which also prevented us from distinguishing between legal and illegal catch. 相似文献
15.
Liesbeth De Vetter Griet Depraetere Colin Janssen Marc Stevens Joris Van Acker 《Annals of Forest Science》2008,65(5):504-504
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17.
Achard P Cheng H De Grauwe L Decat J Schoutteten H Moritz T Van Der Straeten D Peng J Harberd NP 《Science (New York, N.Y.)》2006,311(5757):91-94
Plants live in fixed locations and survive adversity by integrating growth responses to diverse environmental signals. Here, we show that the nuclear-localized growth-repressing DELLA proteins of Arabidopsis integrate responses to independent hormonal and environmental signals of adverse conditions. The growth restraint conferred by DELLA proteins is beneficial and promotes survival. We propose that DELLAs permit flexible and appropriate modulation of plant growth in response to changes in natural environments. 相似文献
18.
Liesbeth Colen Francis Turkelboom Sarah Van Steenwinkel Kasem Al Ahmed Jozef Deckers Jean Poesen 《Land Degradation \u0026amp; Development》2016,27(2):416-426
This paper analyses a local innovation in response to intense soil degradation in olive orchards of north‐west Syria. Farmers developed a practice consisting of quarrying red clayey soil in valley bottoms and applying this soil to hillslope olive orchards with heavily degraded calcareous soils. A biophysical, economic and social analysis of the practice of soil application identified the opportunities and risks of this innovative soil management technique. On the basis of a pairwise comparison of nine adjacent treated and nontreated orchard plots, soil applications were found to increase soil depth by 36%, soil water availability by 28% and total available soil nutrients: potassium (+45%), nitrogen (+12%) and phosphorus (+6%). Olive yield increased by about 40%. A cost–benefit analysis found this practice to be economically viable within a large geographical area, and farmers scored the practice higher than alternative methods. A socio‐economic analysis revealed its widespread adoption among different farmer types. The positive results of soil applications at the farm level explain its fast adoption. However, potential risks – including the further depletion of soil resources and the transfer of soil‐borne diseases – limit the long‐term sustainability of this locally developed practice. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
19.
Bert Noseda Anh Ngoc Tong Thi Lodewijk Rosseel Frank Devlieghere Liesbeth Jacxsens 《Aquaculture International》2013,21(3):709-727
Although the farming of Pangasianodon hypophthalmus is of worldwide economic importance, only little scientific information is currently present on the microbiological quality and safety of these fish fillets throughout the processing chain. This research assessed the microbiological quality and safety of P. hypophthalmus in a Vietnamese Pangasianodon processing company as a case study. The company was initially characterized by a diagnosis of the food safety management system (FSMS) performance by an in-depth interview, which is referred to as a diagnostic instrument. Secondly, a microbiological assessment scheme (MAS), an active and repetitive sampling methodology, was used to evaluate the food safety output and the microbiological quality from raw material until final product. The overall MAS result (level 2) was found to be slightly lower than the company’s food safety performance indicators had indicated (level 2–3). It can be concluded that the microbiological risk of the Vietnamese company and its products is medium high. This is mainly due to the lack of data and knowledge regarding the origin and composition of the microbiological flora present in the fish and in the processing environment. The variability in concentrations of microorganisms also contributed to this lower MAS score and could probably be reduced by improving some specific aspects of the FSMS and the production process. 相似文献
20.
Effect of time and temperature on anticoagulant‐dependent pseudothrombocytopenia in Göttingen minipigs
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