In vivo kinetic study on uptake and distribution of intramuscular tritium-labeled polysulfated glycosaminoglycan in equine body fluid compartments and articular cartilage in an osteochondrial defect model

The uptake and distribution of intramuscularly (IM) administered tritium-labeled polysulfated glycosaminoglycan (³H-PSGAG) in serum, synovial fluid, and articular cartilage of eight horses was quantitated, and hyaluronic acid (HA) concentration of the middle carpal joint was evaluated in a pharmacokinetic study. A full-thickness articular cartilage defect, created on the distal articular surface of the left radial carpal bone of each horse served as an osteochondral defect model. ³H-PSGAG (500 mg) was injected IM, between 14 and 35 days after creation of the defects. Scintillation analysis of serum and synovial fluid, collected from both middle carpal joints at specific predetermined times up to 96 hours post-injection, revealed mean ³H-PSGAG concentrations peaked at 2 hours post-injection. ³H-PSGAG was detected in cartilage and subchondral bone 96 hours post-injection in samples from all eight horses. There were no statistically significant differences in ³H-PSGAG concentration of synovial fluid or cartilage between cartilage defect and control (right middle carpal) joints.

HA assay of synovial fluid revealed concentrations significantly increased at 24, 48, and 96 hours post-injection in both joints. The concentration nearly doubled 48 hours post-injection. However, no statistically significant differences were found between synovial concentrations of HA in cartilage defect and control joints.

³H-PSGAG administered IM to horses, was distributed in the blood, synovial fluid, and articular cartilage. HA concentrations in synovial fluid increased after IM administration of polysulfated glycosaminoglycan.     

鸡新城疫免疫失败的原因及防制措施

1 免疫失败的原因1.1 免疫方式不当。鸡的新城疫(ND)保护性抗体可分为粘膜表面的IgA和血清中的IgG,只有呼吸道粘膜抗体和血清抗体都达到一定滴度时才能有效防止新城疫发生。由于免疫方法选择不当(如饮水免疫),致使鸡的呼吸道粘膜抗体未达到保护水平,或使血清中新城疫抗体不高,都可引起鸡发生新城疫。     

一种澄清的低浓度桑果汁饮料的研制

本研究了桑果汁的澄清方法，确定了一种澄清低浓度桑果汁饮料的配方和工艺，也指出了该饮料的不足以及进一步研究需要解决的问题。     

我国价格体系改革趋势初探

本文以我国国民经济系统与河南省经济子系统的同构性为基础,利用河南省1987年投入产出表,计算出诺依曼最优生产强度和与之相应的最优价格,进而对照现行价格,提出了我国价格体系改革走向的基本构想,并对其合理性、可行性进行了初步研究。     

荔枝采后果皮变色机理研究

对不同采后处理的荔枝在贮藏过程中果皮的渗透率和多酚氧化酶活性变化进行了研究，结果表明：当果皮细胞膜渗透率大于30％时。荔枝果皮就发生大面积褐变；果皮细胞膜渗透率的变化是荔枝采后果皮变色的一个重要指标，而多酚氧化酶活性的高低并不是荔枝果皮颜色变化的决定因素。     

微孢子虫DNA提取方法的比较

本实验采用陈秀改进法、SDS法、CTAB法和TEK改进法对四种微孢子虫SD1、SD2、SD3、SD4基因组DNA进行抽提,并对抽提效果做了对比,结果表明,陈秀改进法对SD1、SD2、SD3基因组DNA抽提效果较好,TEK改进法对SD2、SD3、SD4基因组DNA抽提效果较好,SDS法和CTAB法对四种微孢子虫基因组DNA的抽提效果均不理想,     

利木赞牛改良互助本地黄牛的效果

为了研究利木赞牛对互助黄牛的改良效果,本试验测定了初生、6月龄和1岁利本F1代的体尺、体重,并与本地黄牛进行了比较。结果表明,利本F1代牛的各项被检指标均比本地黄牛有明显的提高(P<0.01)。     

论会计谨慎性原则在茶业企业资产管理中的应用

随着我国成为WT0成员国,茶叶出口政策的调整和我国经济市场化程度日益提高,茶业企业面临各种不确定因素急剧增加,作为会计第一基础要素的资产所受到的冲击更加显著,只有充分估计各种风险和损失,进行合理的资产确认、计量,才能保证财务信息的真实、可靠,避免出现因夸大或缩小资产可能发生的风险造成错误决策,确保茶业企业可持续发展.