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101.
Jianjiang Li Ningning Han Hao Zhang Xiaoyu Xie Yaoyao Zhu E Zhang Jiahui Ma Chuangeng Shang Mengxiong Yin Weidong Xie Xia Li 《Marine drugs》2022,20(9)
Moromycin B (Mor B), saquayamycin B1 (Saq B1), saquayamycin B (Saq B), and landomycin N (Lan N), four angucyclines produced by the marine-derived actinomycete Streptomyces sp., are a class of polyketone compounds containing benzanthracene. Here, the structure–activity relationship of these four compounds was analyzed in human colorectal cancer (CRC) cells. Saq B1, which showed the strongest cytotoxicity with an IC50 of 0.18–0.84 µM for CRC cells in MTT assays, was employed to test underlying mechanisms of action in SW480 and SW620 cells (two invasive CRC cell lines). Our results showed that Saq B1 inhibited CRC cell proliferation in a dose- and time-dependent manner. Notably, lower cytotoxicity was measured in normal human hepatocyte cells (QSG-7701). Furthermore, we observed proapoptosis, antimigration, and anti-invasion activities of Saq B1 in CRC cells. At the same time, the protein and mRNA expression of important markers related to the epithelial–mesenchymal transition (EMT) and apoptosis changed, including N-cadherin, E-cadherin, and Bcl-2, in Saq B1-treated CRC cells. Surprisingly, the PI3K/AKT signaling pathway was shown to be involved in Saq B1-induced apoptosis, and in inhibiting invasion and migration. Computer docking models also suggested that Saq B1 might bind to PI3Kα. Collectively, these results indicate that Saq B1 effectively inhibited growth and decreased the motor ability of CRC cells by regulating the PI3K/AKT signaling pathway, which provides more possibilities for the development of drugs in the treatment of CRC. 相似文献
102.
Weiwen Lin Hanpeng Li Zhiwen Wu Jingyi Su Zehong Zhang Li Yang Xianming Deng Qingyan Xu 《Marine drugs》2022,20(11)
Five new indole diterpenoids named paspaline C–D (1–2) and paxilline B–D (3–5), as well as eleven known analogues (6–16), were identified from fungus Penicillium brefeldianum strain WZW-F-69, which was isolated from an abalone aquaculture base in Fujian province, China. Their structures were elucidated mainly through 1D- and 2D-NMR spectra analysis and ECD comparison. Compound 1 has a 6/5/5/6/6/8 hexacyclic ring system bearing 2,2-dimethyl-1,3-dioxocane, which is rare in natural products. Compound 2 has an unusual open F-ring structure. The cytotoxic activities against 10 cancer cell lines and antimicrobial activities against model bacteria and fungi of all compounds were assayed. No compound showed antimicrobial activity, but at a concentration of 1 μM, compounds 1 and 6 exhibited the highest inhibition rates of 71.2% and 83.4% against JeKo-1 cells and U2OS cells, respectively. 相似文献
103.
The biocontrol fungus Trichoderma harzianum, from both marine and terrestrial environments, has attracted considerable attention. T. harzianum has a tremendous potential to produce a variety of bioactive secondary metabolites (SMs), which are an important source of new herbicides and antibiotics. This review prioritizes the SMs of T. harzianum from 1988 to June 2022, and their relevant biological activities. Marine-derived SMs, especially terpenoids, polyketides, and macrolides compounds, occupy a significant proportion of natural products from T. harzianum, deserving more of our attention. 相似文献
104.
Xinnan Zheng Weizhe Xu Qi Ying Jiajun Ni Xiaoyuan Jia Yanrong Zhou Ting Ye Gongchu Li Kan Chen 《Marine drugs》2022,20(11)
Aphrocallistes vastus lectin (AVL) is a C-type marine lectin derived from sponges. Our previous study demonstrated that oncolytic vaccinia virus carrying AVL (oncoVV-AVL) significantly enhanced the cytotoxicity of oncoVV in cervical cancer, colorectal cancer and hepatocellular carcinoma through the activation of Ras/ERK, MAPK/ERK and PI3K/Akt signaling pathways. In this study, the inflammatory response induced by oncoVV-AVL in a hepatocellular carcinoma cell (HCC) model was investigated. The results showed that oncoVV-AVL increased the levels of inflammatory cytokines including IL-6, IL-8 and TNF-α through activating the AP-1 signaling pathway in HCC. This study provides novel insights into the utilization of lectin AVL in the field of cancer therapy. 相似文献
105.
为明确大豆-玉米带状间作种植模式对点蜂缘蝽Riptortus pedestris发生规律的影响,2023年在山东省大豆单作田中进行诱捕效果试验,筛选诱捕效果最好的诱捕器对黄淮海地区山东、河南和安徽3个省6个市不同种植模式下点蜂缘蝽的发生动态进行监测。结果显示,风叶型诱捕器对点蜂缘蝽的诱捕效果最佳。在大豆单作田内点蜂缘蝽的发生高峰期为7月中旬—8月中旬,此时大豆正值盛花-始荚期。在大豆-玉米带状间作田内点蜂缘蝽的发生动态与大豆单作田的无明显差异,但点蜂缘蝽发生数量及高峰期发生时间可能与杀虫剂的施用时间和次数密切相关。 相似文献
106.
为了阐明大穗型品种豫麦66的灌浆后期衰老特征,本文以小偃22为对照,对两个品种生育后期的衰老以及氮代谢做了研究。结果表明,灌浆后期大穗型品种豫麦66的绿叶面积高于小偃22,MDA含量较小偃22低。其叶片、茎秆以及籽粒的全氮含量均高于小偃22。花前的氮素转运率豫麦66高于小偃22,对于籽粒的贡献率而言,豫麦66花前低于小偃22,花后高于小偃22。因此,大穗型品种豫麦66在生育后期较高的含氮量保证了其后期的高光合作用,进一步提高了产量。 相似文献
107.
坝上沽源县土地利用变化对生态系统服务价值的影响 总被引:7,自引:0,他引:7
分析土地利用变化数据,采用中国陆地生态系统的服务价值测算方法,评估了沽源县土地利用变化引起的生态系统服务价值的变化。结果表明:1990~2006年沽源县耕地面积大幅度减少,牧草地面积稍有减少,林地面积大幅度增加,园地面积没有变化,其余类型面积稍有增加;其中耕地面积减少了73 508.2 hm2,林地面积增加了73 975.5 hm2,这主要是退耕还林草的结果;1990~2006年生态系统服务价值增加了30 550.9万元,其中林地和牧草地对生态系统服务价值的贡献最大。 相似文献
108.
运用统计学方法对祁连山东部武威市的五站近50a的冰雹资料、历史天气图和卫星云图进行了分析,得出了祁连山东部冰雹天气的地理分布规律、气候特征以及冰雹天气产生的环流形势和云图特征,做出了冰雹易发区的冰雹路径图。同时分析了近几年的防雹实例的效果,结果表明:取得了良好的社会和经济效益。 相似文献
109.
本研究对红桔小孢子母细胞减数分裂各时期染色体进行了详细观察。结果表明:细线期、偶线期、粗线期和终变期具有较高的同步性,四分体时期完全一致;染色体行为较为有规律;双线期同源染色体联会构型有棒形、环形、“T”形、“+”字形、环叉形及“3”字形;终变期具9个正常二价体的频率为60%,其余细胞中可观察到单价体、双核、落后染色体、三分体等畸变类型。文章讨论了红桔花粉低育性的遗传学机制。 相似文献
110.
菌丝融合型测定结果表明,32个茭白纹枯病菌菌株隶属于两个菌丝融合群:30个属AG1群,占测定菌株的93.95%,其中AG1-IA、AG1-IC两亚群菌株依次为24与6,有两菌株为AG4,占6.25%。显然,AG1-IA为优势融合群,占测定菌株的75%。融合群致病性测定结果表明,AG1-IA致病性强,AG1-IC弱,AG4基本不致病。茭白与水稻两种纹枯病菌交互接种,其发病基本一致。由此,作者认为这两种纹枯病菌为同一病原物,或者说茭白、水稻是同一病原物的两个寄主植物。 相似文献