Essential oil composition of diploid and tetraploid clones of ginger (Zingiber officinale Roscoe) grown in Australia

Ginger oil, obtained by steam distillation of the rhizome of Zingiber officinale Roscoe, is used in the beverage and fragrance industries. Ginger oil displays considerable compositional diversity, but is typically characterized by a high content of sesquiterpene hydrocarbons, including zingiberene, ar-curcumene, beta-bisabolene, and beta-sesquiphellandrene. Australian ginger oil has a reputation for possessing a particular "lemony" aroma, due to its high content of the isomers neral and geranial, often collectively referred to as citral. Fresh rhizomes of 17 clones of Australian ginger, including commercial cultivars and experimental tetraploid clones, were steam distilled 7 weeks post-harvest, and the resulting oils were analyzed by GC-MS. The essential oils of 16 of the 17 clones, including the tetraploid clones and their parent cultivar, were found to be of substantially similar composition. These oils were characterized by very high citral levels (51-71%) and relatively low levels of the sesquiterpene hydrocarbons typical of ginger oil. The citral levels of most of these oils exceeded those previously reported for ginger oils. The neral-to-geranial ratio was shown to be remarkably constant (0.61 +/- 0.01) across all 17 clones. One clone, the cultivar "Jamaican", yielded oil with a substantially different composition, lower citral content and higher levels of sesquiterpene hydrocarbons. Because this cultivar also contains significantly higher concentrations of pungent gingerols, it possesses unique aroma and flavor characteristics, which should be of commercial interest.     

Rapid harvest schedules and fruit removal as non-chemical approaches for managing spotted wing Drosophila

Spotted wing Drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), has caused significant economic losses to small fruit and berry growers throughout the USA and Europe since its invasion. This pest can lay many eggs over its lifetime within ripening and ripe berries, causing yield loss and the risk of fruit contamination. Zero tolerance for this pest has led to increased use of broad-spectrum insecticides to control it, which are costly and pose many other sustainability and pest management concerns. There is an urgent need to evaluate management strategies that can decrease reliance on chemical controls and mitigate economic losses. Over two growing seasons, we compared harvest schedules for their effect on infestation by D. suzukii, revealing that fruit harvested every 1 or 2 days had significantly fewer D. suzukii larvae than a 3-day harvest schedule. Furthermore, we found that yield per unit effort was highest on a 2-day schedule. Sanitation of the crop is another important component of a successful integrated pest management program, and we found that bagging infested waste berries killed 99% of larvae after 32 h, with higher fruit temperatures in clear bags than white or black bags. In combination, these methods can reduce the effects of this invasive pest on raspberry production. This study will provide guidance to growers on culturally based IPM tactics to decrease reliance on chemical management.     

A quantitative model for trade pathway analysis of plant pest entry and transfer to a host in European Union territory

A quantitative pathway model, QPAFood, has been designed to support risk assessment for plant pest entry into European Union (EU) territory on a range of edible plant commodities via trade flows. The model calculates the distribution of an imported infested/infected commodity along a pathway into and within the EU from source countries, based on Eurostat data and other data/information. The model determines the implications of global trade pathways for the potential arrival of the infested commodity in the EU28 Member States. Within each Member State, the calculation proceeds by distributing the commodity according to uses, notably retail or processing, to the vulnerable area of commercial host crops determined in each NUTS2 region and then quantifies the consequent potential for pest–host contact which could lead to pest transfer. Annual and monthly estimates of contact risk are tabulated and visualized for Member States and NUTS2 regions. The model was developed originally for the European Food Safety Authority using four case studies of specific pest–commodity combinations. These pests had relatively limited host ranges and the model has now been extended in the context of the EC FP7 DROPSA project for the multiple commodity pathways associated with the highly polyphagous fruit pest Drosophila suzukii.     

Rice Germin-Like Proteins: Allelic Diversity and Relationships to Early Stress Responses

Germin-like protein (GLP) markers were associated with quantitative trait loci (QTL) for resistance to the rice blast pathogen, Magnaporthe oryzae in multiple rice (Oryza sativa) mapping populations. Twelve paralogous OsGLP gene family members are located within the physical QTL region on chromosome 8, and gene silencing studies suggest that they contribute collectively to the resistance phenotype. We compared sequence and expression profiles of OsGLP alleles in two resistant and two susceptible parental rice lines to find functional polymorphisms that correlated with the resistant phenotype. Based on coding and promoter sequences, the genes belong to two germin subfamily groups (GER3 and GER4). OsGLP members from both subfamilies were constitutively expressed and developmentally regulated in all cultivars. Transient induction above constitutive levels was observed for some OsGLPs, especially GER4 subfamily members, at early time points after M. oryzae infection and mechanical wounding. Varying 5′ regulatory regions and differential expression of some family members between resistant and susceptible cultivars corresponded with differential hydrogen peroxide (H₂O₂) accumulation after the same stimuli. OsGLP of both GER subfamilies localized to the plant cell wall. The protein location and early gene induction suggest that OsGLPs protect rice leaves at early stages of infection before fungal penetration and subsequent ingress. Our data suggest that regulation of OsGLP genes defines resistant versus susceptible phenotypes.     

The Effect of a Chronic Stressor, Lameness, on Detailed Sexual Behaviour and Hormonal Profiles in Milk and Plasma of Dairy Cattle

The objectives of the present study were to quantify the effects of a biological chronic stressor (lameness) on the duration and frequency of different oestrous behaviours in parallel with milk hormone profiles. Dairy cows 51.8 ± 1.4 days postpartum (n = 59), including 18 non‐lame control cows, were scored for lameness and closely observed for signs of oestrus having had their follicular phases synchronized by administration of gonadotrophin‐releasing‐hormone (GnRH) followed by prostaglandin F_2α (PG) 7 days later. Lameness shortened the period when herd‐mates attempted to mount the lame cows (1.83 ± 0.69 h vs 5.20 ± 1.53 h; p = 0.042) but did not affect the overall duration of total behaviours (lame 12.3 ± 1.3 h vs non‐lame 15.2 ± 1.3 h). Lameness also lowered the intensity of oestrus [1417 ± 206 points (n = 18) vs 2260 ± 307 points (n = 15); p = 0.029]. Throughout the synchronized oestrous period, lame cows mounted the rear of herd‐mates less frequently (p = 0.020) and tended to chin rest less (p = 0.075). Around the period of maximum oestrous intensity, lameness also diminished the proportion of cows mounting the rear of another cow and chin resting (p = 0.048, p = 0.037, respectively). Furthermore, lame cows had lower progesterone values during the 6 days before oestrous (p ≤ 0.05). Fewer lame cows were observed in oestrus following PG (non‐lame 83%, lame 53%; p = 0.030); however, if prior progesterone concentrations were elevated, lame cows were just as likely to be observed in oestrus. In conclusion, following endogenous progesterone exposure, lameness shortens the period when herd‐mates attempt to mount lame cows but does not affect the incidence of oestrous. However, lame cows are mounted less frequently and express oestrus of lower intensity. This is associated with lower progesterone prior to oestrus but not with abnormal oestradiol or cortisol profiles in daily milk samples.     

Integrating advanced technologies for optimization of aerial herbicide applications

Aerial application of herbicides continues to be a dominant method of vegetation control in Canadian forest management. In this paper, we describe a suite of relatively modern technologies and emphasize their potential for integrated use in optimization of aerial herbicide treatments. The potential is illustrated using several case studies involving fixed-wing applications of glyphosate-based herbicide in the boreal forest region of Ontario, Canada. Results indicate that integration of geographic information systems, differential global positioning, electronic-guidance, on-site meteorological monitoring and remote sensing can be used to effectively plan, enhance application control and provide detailed post-treatment assessment and archival data for herbicide spray programs. SprayAdvisor, a GIS-based decision support system with the capacity to directly integrate all of these elements is briefly described.     

Phytophthora infestans sporangial germination and tuber rot reduced by soil residues of fungicides

Foliar sprays of Difolatan were equal to or slightly better than those of maneb for the control of late blight when applied at equivalent rates of active ingredient. Difolatan foliar sprays, in general, resulted in less late blight tuber rot than did maneb sprays. Foliar sprays of Bordeaux mixture or Difolatan produced residues of these fungicides in the soil. These residues were greater in the surface soil (0 to 1/2 inch depth) than in the 1 to 2 inch layer, but were not sufficient to inhibit indirect germination of the sporangia ofP. infestans in soil leachates. Similar results were obtained with applications of Bordeux mixture or Difolatan to the soil in the absence of any foliar spray. Residues from foliar sprays of Difolatan plus soil treatments of Difolatan or Bordeaux mixture, made before and after the last cultivation, inhibited indirect germination ofP. infestans sporangia in soil leachates from the surface soil, but not from the 1 to 2 inch layer of soil. Application of foliar sprays of Bordeaux mixture to plots given soil treatments of Difolatan before the last cultivation resulted in fungitoxic residues in the surface soil and in the 1- to 2-inch layer of soil, but not when Difolatan soil treatments were made after the last cultivation. Similar results were obtained when maneb foliar sprays were applied to plots that had received soil applications of Difolatan.