Isolation of methicillin-resistant Staphylococcus aureus from the environment in a veterinary teaching hospital

After recognition of a cluster of methicillin-resistant Staphylococcus aureus (MRSA) infection in horses and humans at the Ontario Veterinary College Veterinary Teaching Hospital, environmental contamination with MRSA was evaluated. A total of 260 environmental surfaces were sampled during periods when MRSA-infected horses were hospitalized, and MRSA was isolated from 25/260 sites (9.6%). The most commonly contaminated sites were stalls housing MRSA-positive horses, but other stalls, medical equipment, personal items, and equine restraint devices also were contaminated. The role of the environment in the transmission of MRSA infection to horses or humans is unclear. However, relatively widespread contamination of the hospital environment, as apparently occurs when infected horses are hospitalized, suggests that the environment may be an important source of MRSA infection. This possibility must be taken into consideration when designing infection control and disinfection protocols.     

Spatial and temporal patterns of vegetation recovery following sequences of forest fires in a Mediterranean landscape,Mt. Carmel Israel

The Mediterranean ecosystem of Mt. Carmel is subjected to increasing number of forest fires at various extents and severities due to increasing human activities. Accordingly, we tested whether in areas exposed to different fire histories vegetation regeneration is different in north versus south facing slopes, and the potential impact on erosion processes. Using remote sensing techniques we evaluated the Enhanced Vegetation Index (EVI) to monitor vegetation recovery following a single fire and three successive fires, using a series of Landsat images taken between 1985–2002. Following a single fire, vegetation cover reached pre-disturbance values within less than 5 years. Repeated fires caused further reduction of EVI values, especially at south facing slopes (SFS). The effects of three successive fires within 10 years, followed by a three year recovery period, however, are negligible when considering vegetation cover values. This was deduced as north facing slope EVI values returned to pre-disturbance conditions at the end of the 3 years and SFS EVI values to 80% of the pre-disturbance conditions. Our results indicate that Mediterranean eco-geomorphic systems are quite resilient, showing quick response, at least in terms of return to pre-disturbance states of vegetation cover, and hence of soil erosion rates. This is true not only in response to a disturbance caused by a single fire, but also for repetitive fire incidents.     

Regulation of lysine metabolism and endosperm protein synthesis by the opaque-5 and opaque-7 maize mutations

Two high lysine maize endosperm mutations, opaque-5 (o5) and opaque-7 (o7), were biochemically characterized for endosperm protein synthesis and lysine metabolism in immature seeds. Albumins, globulins, and glutelins, which have a high content of lysine, were shown to be increased in the mutants, whereas zeins, which contain trace concentrations of lysine, were reduced in relation to the wild-type lines B77xB79+ and B37+. These alterations in the storage protein fraction distribution possibly explain the increased concentration of lysine in the two mutants. Using two-dimensional polyacrylamide gel electrophoresis of proteins of mature grains, variable amounts of zein polypeptides were detected and considerable differences were noted between the four lines studied. The analysis of the enzymes involved in lysine metabolism indicated that both mutants have reduced lysine catabolism when compared to their respective wild types, thus allowing more lysine to be available for storage protein synthesis.     

Ultrasonographic imaging of abomasal milk clotting and abomasal diameter in healthy and diarrheic calves

In case of diarrhea calves are treated with oral rehydration solutions (ORS), which are known to increase abomasal pH and inhibit milk clotting in vitro. Nevertheless, recent studies have shown that ORS with HCO₃^‐ ≤ 62 mmol/L do not interfere with abomasal milk clotting in healthy calves. However, in diarrheic calves, feeding ORS and milk simultaneously may disturb abomasal curd formation and exacerbate diarrhea due to faster abomasal passage of ingesta. Therefore, the aim of the present study was to ultrasonographically examine abomasal milk clotting and diameter after feeding milk and milk replacer (MR) with and without ORS to healthy and diarrheic calves. Abomasal curd formation and diameter in healthy and diarrheic calves were ultrasonographically imaged before and after feeding milk, MR and ORS prepared in milk or MR. Feeding mixtures of milk or MR with ORS did not cause any remarkable differences in the ultrasonographic images of abomasal content. Moreover, abomasal milk clotting was not disturbed due to diarrhea. Statistically significant differences of abomasal diameter after feeding between healthy and diarrheic calves indicated that abomasal emptying is delayed in diarrheic calves. Hence, further studies are needed to determine reasons for decelerated abomasal passage in calves suffering from diarrhea.     

Identification and characterization of H2N3 avian influenza virus from backyard poultry and comparison with novel H2N3 swine influenza virus

In early 2007, H2N3 influenza virus was isolated from a duck and a chicken in two separate poultry flocks in Ohio. Since the same subtype influenza virus with hemagglutinin (H) and neuraminidase (N) genes of avian lineage was also identified in a swine herd in Missouri in 2006, the objective of this study was to characterize and compare the genetic, antigenic, and biologic properties of the avian and swine isolates. Avian isolates were low pathogenic by in vivo chicken pathogenicity testing. Sequencing and phylogenetic analyses revealed that all genes of the avian isolates were comprised of avian lineages, whereas the swine isolates contained contemporary swine internal gene segments, demonstrating that the avian H2N3 viruses were not directly derived from the swine virus. Sequence comparisons for the H and N genes demonstrated that the avian isolates were similar but not identical to the swine isolates. Accordingly, the avian and swine isolates were also antigenically related as determined by hemagglutination-inhibition (HI) and virus neutralization assays, suggesting that both avian and swine isolates originated from the same group of H2N3 avian influenza viruses. Although serological surveys using the HI assay on poultry flocks and swine herds in Ohio did not reveal further spread of H2 virus from the index flocks, surveillance is important to ensure the virus is not reintroduced to domestic swine or poultry. Contemporary H2N3 avian influenza viruses appear to be easily adaptable to unnatural hosts such as poultry and swine, raising concern regarding the potential for interspecies transmission of avian viruses to humans.     

Phytophthora hydropathica,a new pathogen identified from irrigation water,Rhododendron catawbiense and Kalmia latifolia

A new species of Phytophthora, previously referred to as taxon Dre II, is named Phytophthora hydropathica. It is heterothallic, but all isolates recovered to date are of the A1 compatibility type. Plerotic oospores are produced. Its sporangia are usually obpyriform and are nonpapillate and noncaducous. Isolates of P. hydropathica had nearly identical single‐strand conformation polymorphism (SSCP)‐based DNA fingerprints that are distinct from those of all existing species. Their closest relatives are P. parsiana and P. irrigata. This new species is able to grow at relatively high temperatures, with an optimum of 30°C and a maximum of 40°C. It was frequently isolated from irrigation water during warm summers. This species caused leaf necrosis and shoot blight of Rhododendron catawbiense and collar rot of Kalmia latifolia at two nurseries where irrigation reservoirs yielded P. hydropathica. Its potential impact on other horticultural crops is discussed.     

Variability in the El Niño-Southern Oscillation through a glacial-interglacial cycle

The El Ni?o-Southern Oscillation (ENSO) is the most potent source of interannual climate variability. Uncertainty surrounding the impact of greenhouse warming on ENSO strength and frequency has stimulated efforts to develop a better understanding of the sensitivity of ENSO to climate change. Here we use annually banded corals from Papua New Guinea to show that ENSO has existed for the past 130,000 years, operating even during "glacial" times of substantially reduced regional and global temperature and changed solar forcing. However, we also find that during the 20th century ENSO has been strong compared with ENSO of previous cool (glacial) and warm (interglacial) times. The observed pattern of change in amplitude may be due to the combined effects of ENSO dampening during cool glacial conditions and ENSO forcing by precessional orbital variations.