Preliminary results of differentiating apple sports by pollen ultrastructure

The pollen grain surface morphology of two Red Delicious apple (Malus × domestica Borkh.) sports, Aversang and Ultrared, and two Gala apple sports, Galalea and Splenola, were examined using scanning electron microscopy for genotype differentiation. Quantitative data on pollen dimensions, ridge patterns, and pore dimensions were extracted from micrographs using image analysis. Data were examined with analysis of variance, canonical variate analysis, and discriminant analysis in order to differentiate genotypes. The combination of image analysis to extract quantitative data and multivariate analysis was successful in differentiating apple sports with exine pore and pollen grain measurements. This revised version was published online in July 2006 with corrections to the Cover Date.     

Manova and discriminant analyses of phenotypic data as a guide for parent selection in kiwifruit (Actinidia deliciosa) breeding

Evaluation of seedling populations assists in identifying those plants most desirable as parents for a breeding programme. A multivariate analysis of variance (Manova) showed that the seedling populations from 6 kiwifruit (Actinidia deliciosa) crosses differed in their overall merit. Discriminant functions were then used to identify the characters most powerful in distinguishing between populations. The populations were placed in order of their function scores which represent overall merit, and the superior populations were determined. The results showed that `Bruno' was a superior female parent for the production of seedlings with high discriminant function scores for floriferousness (male offspring)and productivity and high vitamin C content (female offspring). One male parent D-1-20 was consistently superior to D-1-6 the other male parent. This revised version was published online in July 2006 with corrections to the Cover Date.     

Genetic variation in stomatal characteristics and behaviour,water use and growth of five Vicia faba L. genotypes under contrasting soil moisture regimes

Summary Five genotypes of Vicia faba L. with contrasting frequencies were grown in a controlled environment in soil moisture regimes of 100%, 78% and 61% of field capacity. Growth, water use and leaf conductance characteristics were measured. Stomata were more frequent on abaxial than adaxial surfaces, the abaxial/adaxial ratio ranging from 1.12 to 1.34. There were significant (P<0.001) correlations between calculated stomatal conductance, based on measurements of stomatal frequency and length, and leaf conductance measured with a diffusion porometer, in the two higher soil moisture treatments. In the 100% regime only, abaxial stomata opened wider than adaxial. Ranking of the genotypes for leaf conductance and plant transpiration was similar in all three regimes with large leaved types having the lowest conductance and greatest water use efficiency. Growing plants with reduced soil moisture availability improved water use efficiency, the ranking for which was also similar in all treatments. Relative growth rate and net assimilation rate were greatest with full watering, less in the 78% and least in the 61% regime. Leaf area ratio was less affected by available soil moisture and only in some genotypes.     

Tropical tree community shifts: Implications for wildlife conservation

In tropical forest systems tree community change after initial succession (approximately 50-100 years) is very difficult to detect because of the very slow pace of transformation. Since the mid 1980s, there has been an accumulation of evidence that many forests traditionally considered old growth or mature forests have been disturbed. Using 18 years of data on forest change in Kibale National Park, Uganda, we tested the following hypotheses. Species that frequently recruit only into areas of large-scale disturbance (e.g., conversion to agriculture) (1) have a more strongly negative annualized rate of population change (i.e., recruitment is less than mortality) than trees recruiting into the understory or canopy treefall gaps and (2) these species are declining in their average cumulative diameter at breast height (DBH). Both hypotheses were verified. We then examined relationships between forest change and diets of the five diurnal primates in Kibale. The emergent patterns suggest that forest change will lead to declines in some species, particularly the black-and-white colobus (Colobus guereza). We concluded that what was considered mature forest in Kibale has actually been disturbed in the recent past, and we discuss how potential sources of disturbance (dry-periods, elephant population fluctuations, and human disturbance) may affect both forest change and animal populations. We assess how such information might be useful in forest management.     

Genomic Imprinting in Canis familiaris

For the vast majority of mammalian genes, maternally- and paternally-derived alleles behave identically and are either expressed or repressed, regardless of whether they were inherited from egg or sperm. For imprinted genes, however, this is not the case. The alleles of imprinted genes are epigenetically modified in a parent-of-origin-specific manner and, as a consequence, maternally- and paternally-derived alleles behave differently. Typically one allele is expressed while the other is silent. Although relatively few in number, imprinted genes are the focus of intensive study, as they have important roles in embryonic development. Abnormal expression of imprinted genes results in growth disorders and is implicated in several clinical conditions. Most studies of imprinted genes have been performed in rodents or primates, with limited studies in other mammals such as bovine and opossum. We have recently demonstrated the existence of imprinted genes in the canine, by showing that the canine insulin-like growth factor 2 receptor gene ( IGF2R ) is monoallelically expressed, with predominant expression of the maternally-derived allele and repression of the paternally-inherited allele. Our ultimate goal is to characterize all imprinted genes in the canine, and to understand how they contribute to canine reproduction, development and disease. Such knowledge will be vital for optimizing the success of most reproductive strategies in the canine.     

Genotypic diversity in field isolates of Babesia bovis from cattle with babesiosis after vaccination

Objective To determine whether particular genotypes of Babesia bovis were common to field isolates obtained from cattle properties in Queensland where the B bovis vaccine had apparently failed.
Design A comparative study of polymerase chain reaction genotypes in different populations of B bovis .
Procedure Two polymerase chain reaction assays were applied to analyse DNA extracts of B bovis vaccine (K, T and Dixie strains) and 27 field isolates from 24 properties where disease outbreaks had occurred despite the use of the vaccine. To evaluate the stability of the genotypes identified, 11 of the field isolates were inoculated into experimental cattle that had either been previously vaccinated with T strain or not vaccinated.
Results No particular genotype of B bovis was responsible for the problems observed in previously vaccinated herds. None of the isolates had genotypes identical to the vaccine strains used. No geographic trends among the genotypes were observed. Isolates that originated from the same property also had different genotypes. Blood passage of the 11 field isolates in either previously vaccinated or nonvaccinated cattle did not alter the original genotype.
Conclusion No particular genotypes identified by the Bv80 and BvVA1 polymerase chain reaction assays could be associated with vaccine failures.     

Post-Partum Involution of the Canine Uterus – Gross Anatomical and Histological Features

We aimed to study the normal puerperium in the bitch. Ovariohysterectomy was performed in nine bitches, each at a different day after normal whelping; their genital tract was subject to gross anatomical examination, as well as to histological examination and electron microscopy scanning. Corpora albicans were evenly distributed in the left and right ovaries and placental sites were evenly distributed among left and right uterine horns. Placental sites were initially of dark green to grey colour, later becoming dark brown; their length and height progressively decreased. Height of the myometrium and diameter of the uterine glands progressively decreased. Trophoblast-like cells were consistently observed at the placental sites and on the surface of the interplacental areas, at all time points where hysterectomy had been performed. It is suggested that involution of the canine genital tract can last up to 3 months and is slow. Continuous (up to D84 post-partum) presence of prominent placental sites should be considered a normal feature of canine uterine post-partum involution.     

Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato

Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two-hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.     

温度对荔枝开花的影响

低温能促进荔枝（LitchichinestsSonn．）开花,但是成功诱导开花的临界温度和所需低温量并不清楚。研究了最高温／最低温：15°／5°,20°／5°,20°／10°,20°／15°,25°／10°,25°／15°,25°／20°和30°／20℃8个温度处理对KwaiMayPink荔枝和Casino荔枝营养生长和开花的影响。在第2个试验中,WaiChee荔枝在转移到无诱导条件的30°／20℃温度前,分别给予0,2,4,6,8,10和31周的15℃低温诱导。在第3个试验中．WaiChee荔枝生长温度为15℃时,每24小时内给予高于20℃的温度处理时间分别为0,1,2和8h（15℃以上分别为0,2,4和15h）。在第1个试验中,KwaiMayPink的营养生长温度为25°／20℃和30°／20℃,温度为25°／10℃和25°／15℃时,既有营养生长,又有花穗发育,较低的温度更利于开花。Casino荔枝除了在25°／10℃和25°／15℃的温度条件下既无营养生长,也无花穗发育外,其余处理的反映和KwaiMayPink荔枝类似。对2个荔枝品种而言,在20°／15℃和更低的温度时,几乎所有枝条都开了花。‘WaiChee’荔枝成花诱导条件为在转移到高温条件下之前,保持15℃的低温4周,最长6周。低温时间的长短还影响花穗的类型,15℃的低温诱导10周后,‘WaiChee’荔枝几乎无有叶花穗。保持15℃的低温     

Evidence for large earthquakes in metropolitan los angeles

The Sierra Madre fault, along the southern flank of the San Gabriel Mountains in the Los Angeles region, has failed in magnitude 7.2 to 7.6 events at least twice in the past 15,000 years. Restoration of slip on the fault indicated a minimum of about 4.0 meters of slip from the most recent earthquake and suggests a total cumulative slip of about 10.5 meters for the past two prehistoric earthquakes. Large surface displacements and strong ground motions resulting from greater than magnitude 7 earthquakes within the Los Angeles region are not yet considered in most seismic hazard and risk assessments.