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101.
R. Yadav K. Mohan V. Kumar M. Sarkar K. Nitu H.H.D. Meyer B.S. Prakash 《Domestic animal endocrinology》2013
A highly sensitive enzyme immunoassay (EIA) that used the second antibody coating technique and the cortisol-horseradish peroxidase conjugate as a label for determination of free and total cortisol in blood plasma of dairy animals (cows, buffaloes, and goats) was developed. For biological validation of the EIA, blood samples were collected from the animals at 48 and 24 h before and 0, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, and 132 h after dexamethasone administration. The EIA was performed directly with 20 μL of fresh plasma (for free cortisol) and also with 20 μL of heat-treated plasma (for total cortisol) after 1:5 dilutions with PBS. Cortisol standards ranging from 0.39 to 200 pg/well/20 μL were used, and the sensitivity of the EIA procedure was found to be 0.39 pg/well/20 μL, which corresponded to 0.02 ng/mL. In comparison with RIA the EIA was at least 4 times more sensitive and required 5 times less cortisol antiserum. In female cattle, buffaloes, and goats, the total, free, and bound plasma cortisol before dexamethasone administration was significantly (P < 0.05) higher than the total, free, and bound cortisol after dexamethasone administration. It can be concluded from these studies that the direct, sensitive EIA validated for estimating the free and total cortisol concentrations was sufficiently reliable and quick for studying the dynamics of cortisol distribution in blood plasma of dairy animals. 相似文献
102.
A Yadav KP Singh MK Singh N Saini P Palta RS Manik SK Singla RC Upadhyay MS Chauhan 《Reproduction in domestic animals》2013,48(5):858-865
For investigating the effects of physiologically relevant heat shock, buffalo oocytes/embryos were cultured at 38.5°C (control) or were exposed to 39.5°C (Group II) or 40.5°C (Group III) for 2 h once every day throughout in vitro maturation (IVM), fertilization (IVF) and culture (IVC). Percentage of oocytes that developed to 8‐cell, 16‐cell or blastocyst stage was lower (p < 0.05) and the number of apoptotic nuclei was higher (p < 0.05) for Group III > Group II > controls. At both 8–16‐cell and blastocyst stages, relative mRNA abundance of stress‐related genes HSP 70.1 and HSP 70.2 and pro‐apoptotic genes CASPASE‐3, BID and BAX was higher (p < 0.05) in Groups III and II than that in controls with the exception of stress‐related gene HSF1. Expression level of anti‐apoptotic genes BCL‐XL and MCL‐1 was also higher (p < 0.05) in Groups III and II than that in controls at both 8–16‐cell and blastocyst stages. Among the genes related to embryonic development, at 8–16‐cell stage, the expression level of GDF9 was higher (p < 0.05) in Group III than that in controls, whereas that of GLUT1, ZAR1 and BMP15 was not significantly different among the three groups. At the blastocyst stage, relative mRNA abundance of GLUT1 and GDF9 was higher (p < 0.05) in Group II than that in controls, whereas that of ZAR‐1 and BMP15 was not affected. The results of this study demonstrate that exposure of buffalo oocytes and embryos to elevated temperatures for duration of time that is physiologically relevant severely compromises their developmental competence, increases apoptosis and affects stress‐, apoptosis‐ and development‐related genes. 相似文献
103.
Yadvinder Singh E. Humphreys S.S. Kukal B. Singh Amanpreet Kaur S. Thaman A. Prashar S. Yadav J. Timsina S.S. Dhillon N. Kaur D.J. Smith P.R. Gajri 《Field Crops Research》2009
Raised beds are widely used in agriculture in developed countries and have proven to be an excellent option for wheat. Permanent raised beds may also offer benefits for rice–wheat (RW) systems in South Asia, in terms of both production and the possibility that furrow-irrigation may be more efficient than flood irrigation. The performance of a RW system on permanent raised beds (37 cm wide, 15 cm high, furrow width 30 cm) was compared with conventional cultivation on the flat on sandy loam and loam soils in replicated experiments in central Punjab, India. The experiments commenced with wheat sown in November 2002, and were continued for 8 crops. 相似文献
104.
Dev K Gautam SK Giri SK Kumar A Yadav A Verma V Kumar P Singh B 《Research in veterinary science》2012,93(2):743-748
Heterogeneous amniotic fluid contains various cell types. The aim of this study was to characterize and differentiate some of the key stemness attributes of the amniotic fluid-derived cells in buffalo (Bubalus bubalis). The amniotic fluid (AF) cells were cultured without feeder cells, in DMEM containing 15% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin/ampicillin, 1% vitamin solution, and 1% l-glutamine in 5% CO(2) in humidified air at 38.5±0.5 °C. After 6 days of culture different types of cells viz., star shaped (62.7%), spherical without nucleus (1.9%), spherical with nucleus (26.4%), pentagonal (0.4%), and free floating/rounded cells (8.3%) were observed. Most of the cells started anchorage-dependent growth after day 7 of the culture. Expression of alkaline phosphatase (AP) and Oct-4, Nestin and FGF-5 were observed from the AF cells at different passages. Using species-specific primers, a PCR amplicon of 200, 296 and 210 bp were observed for Oct-4, Nestin and FGF-5, respectively. The cells were found to have a normal karyotype at different passages. These results may contribute towards establishing non-embryonic pluripotent stem cells for various therapeutic and reproductive biotechnological applications in the species. 相似文献
105.
106.
Singh K Rani A Kumar S Sood P Mahajan M Yadav SK Singh B Ahuja PS 《Tree physiology》2008,28(9):1349-1356
Tea (Camellia sinensis (L.) O. Kuntze) leaves are a major source of flavonoids that mainly belong to the flavan 3-ols or catechins. Apart from being responsible for tea quality, these compounds have medicinal properties. Flavanone 3-hydroxylase (F3H) is an abundant enzyme in tea leaves that catalyzes the stereospecific hydroxylation of (2S)-naringenin to form (2R,3R)-dihydrokaempferol. We report a full-length cDNA sequence of F3H from tea (CsF3H Accession no. AY641730). CsF3H comprised 1365 bp with an open reading frame of 1107 nt (from 43 to 1149) encoding a polypeptide of 368 amino acids. Expression of CsF3H in an expression vector in Escherichia coli yielded a functional protein with a specific activity of 32 nmol min(-1) mg protein(-1). There was a positive correlation between the concentration of catechins and CsF3H expression in leaves of different developmental stages. CsF3H expression was down-regulated in response to drought, abscisic acid and gibberellic acid treatment, but up-regulated in response to wounding. The concentration of catechins paralleled the expression data. Exposure of tea shoots to 50-100 microM catechins led to down-regulation of CsF3H expression suggesting substrate mediated feedback regulation of the gene. The strong correlation between the concentration of catechins and CsF3H expression indicates a critical role of F3H in catechin biosynthesis. 相似文献
107.
Shobh Nath Yadav Omkar Kumar A. M. Latare Manish Kumar 《Communications in Soil Science and Plant Analysis》2017,48(10):1145-1153
Pot culture experiment was conducted to evaluate the suitability of extractants and to determine the critical limit of boron (B) in soil and mustard plant in Inceptisols of Varanasi. Twenty-one bulk soil collected from different locations were used for growing mustard. Five extractants, namely hot water, hot 0.01molar (M) calcium chloride (CaCl2), 0.01M CaCl2 + 0.05 M mannitol, 1.0 M ammonium acetate (NH4OAC) and 0.05 M hydrochloric acid (HCl), were assessed by correlating the amount of extractable B in untreated and B fertilizer-treated soil with Bray’s per cent yield, plant tissue B concentration and B uptake by mustard. Similarly, correlation coefficients of the B extracted by different extractants and soil properties were calculated. The suitability of B extracted by different extractants was in the order of hot 0.01M CaCl2 (HCC-B) > hot water (HW-B) > 1.0 M NH4OAC (AA-B) > 0.05M HCl (HA-B) > 0.01M CaCl2 + 0.05M mannitol (CCM-B). The critical limits of extractable B in soil as determined by the graphical procedure were 0.54, 0.60, 0.36, 0.45 and 0.45 mg kg?1 and the statistical procedures were 0.54, 0.60, 0.38, 0.46 and 0.48 mg kg?1 with HW-B, HCC-B, CCM-B, AA-B and HA-B, respectively. Soil containing available B below the critical limit responded to B fertilization. 相似文献
108.
Amit Singh Yadav Deepak Bhatnagar 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(1):18-24
The spices used in the Indian foods such as Star anise (Illicium verum), Bay leaves (Cinnamomum zeylanicum) and Cobra’s saffron (Mesua ferrea), and Acacia (Acacia catechu), which have medicinal value, were used as test samples, to find their effect on in vitro lipid peroxidation (LPO). Rat liver post mitochondrial supernatant (PMS) in Tris HCl buffer, pH 7.4 was incubated for 0 and
1 h, with various test extracts in three different oxidant systems. The results show that addition of test samples to FeCl3 medium at 0 h significantly stop the initiation of the LPO. However, the propagation phase of LPO was inhibited by Cobra’s
saffron and Acacia and not by Star anise and Bay leaves. The test samples also showed strong reducing power and superoxide
radical scavenging activity. Cobra’s saffron and Acacia showed the highest antioxidant activity, probably due to the higher
polyphenol content as compared to other test samples. 相似文献
109.
110.
Rashmi Aggarwal Aradhika Tripathi Anita Yadav 《European journal of plant pathology / European Foundation for Plant Pathology》2010,128(3):333-342
Tilletia indica Mitra is the causal agent of Karnal bunt of wheat, an important disease prevalent in several countries. The disease is internationally
quarantined and the pathogen due to its heterothallic nature shows high variability. In the present study, we compared the
pathogenic behaviour of various isolates of T. indica collected from different geographical locations of India and genetically characterized monosporidial (Ms) culture lines raised
from these isolates of the pathogen. Pathogenic variability revealed existence of three pathotypes based on aggressiveness
on a set of differential host genotypes. Monosporidial culture lines viz., 5 each from KB1, KB2, KB4 and KB5 and three lines
of KB3 were established and analyzed genetically using 12 Universal Rice Primers (URPs). Amplification showed 98.44% polymorphism
and primer URP 13R produced 100% polymorphic bands. Maximum similarity (83%) was between KB1MsB and KB1MsD as calculated by
Jaccard’s similarity coefficient, whereas, minimum similarity was between KB1MsC and KB4MsB; KB1MsE and KB3MsA (46%). Three
groups were formed among all Ms culture lines. One major group consisted of 13 lines with approximately 70% similarity, the
second group consisted of 7 culture lines showing 55% similarity and the third group consisted of 3 Ms lines. URPs were able
to differentiate the Ms culture lines raised from different T. indica isolates and the results indicated heterogeneity in the pathogen population. 相似文献