Metabolism of the olive oil phenols hydroxytyrosol, tyrosol, and hydroxytyrosyl acetate by human hepatoma HepG2 cells

To study the potential hepatic metabolism of olive oil phenols, human hepatoma HepG2 cells were incubated for 2 and 18 h with hydroxytyrosol, tyrosol, and hydroxytyrosyl acetate, three phenolic constituents of olive oil. After incubation, culture media and cell lysates were hydrolyzed with beta-glucuronidase and sulfatase and analyzed by LC-MS. In vitro methylation, glucuronidation, and sulfation of pure phenols were also performed. Methylated and glucuronidated forms of hydroxytyrosol were detected at 18 h of incubation, together with methylglucuronidated metabolites. Hydroxytyrosyl acetate was largely converted into free hydroxytyrosol and subsequently metabolized, yet small amounts of glucuronidated hydroxytyrosyl acetate were detected. Tyrosol was poorly metabolized, with <10% of the phenol glucuronidated after 18 h. Minor amounts of free or conjugated phenols were detected in cell lysates. No sulfated metabolites were found. In conclusion, olive oil phenols can be metabolized by the liver as suggested by the results obtained using HepG2 cells as a hepatic model system.     

Carbohydrate composition of selected plum/prune preparations

Eighteen plum/prune preparations and byproducts were analyzed for proximate constituents and carbohydrate profiles. Plum puree and prune juice contained the highest concentrations of ash (13.0 and 13.8%, respectively). Crude protein (CP), acid-hydrolyzed fat (AHF), and total dietary fiber (TDF) concentrations were higher in byproducts (waste cake and dried plum pits) compared with the other fractions. Several classes of oligosaccharides were found in low concentrations in many of the substrates and were associated with the fruit rather than the pit. Maltooligosaccharides were found in very high concentrations in three of the preparations as a result of the addition of maltodextrin during processing. Monosaccharides, sugar alcohols, and oligosaccharides were found in higher concentrations in the fruit than in the pit and accounted for 2.9-84.7% of substrate organic matter. These results indicate that carbohydrates of various types constitute a significant proportion of plum/prune preparations and byproducts.     

Terpene compounds as possible precursors of 1,8-cineole in red grapes and wines

While the contribution of 1,8-cineole to the aroma of wine has been reported, it is a matter of controversy that the vineyards producing such wines are surrounded by Eucalyptus trees, which may contribute their essence to the grapes. However, experimental information presented in this paper suggests that 1,8-cineole can be produced by chemical transformation of limonene and alpha-terpineol, and this process may be responsible for the occurrence of Eucalyptus-like aroma in Tannat wines from vines not grown in the vicinity of Eucalyptus trees. A mechanism for the chemical transformation of these aroma compounds is proposed.     

Long-term effects of a single compaction by heavy field traffic on yield and nitrogen uptake of annual crops

The long-term effects of soil compaction by heavy traffic on crop growth were examined in field experiments on a heavy clay (Vertic Cambisol) and an organic soil (Mollic Gleysol). There were three treatments: one pass and four repeated passes with a tandem axle load of 16 Mg, with wheel tracks completely covering the plot area, and a control without experimental traffic. Both loadings compacted the soils to a depth of 0.4–0.5 m. For 9 years after the loading, spring cereals (oats, wheat and barley) were the main crops grown. Yield, moisture content at harvest, thousand-kernel and bulk weight and nitrogen uptake of crops were determined each year. Although lodging of crops in the control and sometimes also in the treatment with one pass complicated the interpretation of results, especially for the organic soil, compaction clearly did affect crop production. For several years after the loading, it decreased yields and nitrogen uptake of crops and lowered seed moisture contents at harvest. Effects of the compaction were especially marked on the clay soil in the first 3 years and the rainy sixth year. Taken as a mean of the first 8 years, compaction of the clay soil with four passes reduced the yields by 4% and nitrogen uptake of annual crops by 9%. Compaction of the organic soil with four passes decreased the yield by 1% and nitrogen yield by 4%, as a mean of the first 5 and the last 3 years. The bulk weight or the thousand-kernel weight of yields was not notably affected by the compaction.     

Spatial and temporal controls of soil respiration rate in a high-elevation, subalpine forest

We examined soil respiration to determine what measurable environmental variables can be used to predict variation in soil respiration rates, spatially and temporally, at a high-elevation, mixed conifer, subalpine forest site at the Niwot Ridge Ameriflux Site in Colorado. For three summers, soil respiration rates were measured using soil collars and a portable gas-exchange system. Transects of the collars were established to ensure spatial characterization of the litter-repleted areas beneath tree crowns and the litter-depleted open spaces between tree crowns. Soil temperature and soil moisture were both identified as important drivers of soil respiration rate, but were found to confound each other and to function as primary controls at different scales. Soil temperature represents a primary control seasonally, and soil moisture represents a primary control interannually. Spatially, organic layer thickness, ammonium concentration, water content, and the microbial and soil soluble carbon pools were found to predict variation from point to point. Soil microbial biomass strongly correlated to soil respiration rate, whereas root biomass was identified as a weak predictor of respiration rate and only when controlling for other variables. Spatial variation in soil respiration rate is highly determined by the depth of the soil organic horizon, which in this ecosystem varies predictably according to distance from trees. The conclusions that can be drawn from the study provide the foundation for the development of future models of soil respiration driven by fundamental variables of the climate and soil microenvironment.     

Nutritional value of cherry tomatoes (Lycopersicon esculentum Cv. Naomi F1) harvested at different ripening stages

The average content of some classes of antioxidants is generally higher in cherry tomatoes than in normal-sized berries. The aim of this work was to assess the nutritional value of cherry tomato (cv. Naomi F1) by investigating the compositional pattern of berries harvested at different ripening stages and evaluating, in particular, all of the main antioxidants (carotenoids, ascorbic acid, phenolic compounds, and alpha-tocopherol) and the antioxidant activity of the water-soluble and water-insoluble fractions. Results confirmed the relatively high level of carotenoids in cherry tomato but showed that not all biologically active compounds necessarily increase in tomatoes picked at later stages of ripeness. Cherry tomatoes harvested at full ripeness exhibited the highest level of carotenoids and antioxidant activity in the water-insoluble fraction. On the other hand, no significant differences in ascorbic acid content were observed at different ripening stages, whereas the main phenolics content and the antioxidant activity of water-soluble fraction showed slight, but significant, decreases at later stages of ripeness.     

Quantitation of carcinogenic heterocyclic aromatic amines and detection of novel heterocyclic aromatic amines in cooked meats and grill scrapings by HPLC/ESI-MS

A tandem solid-phase extraction method was used to isolate carcinogenic heterocyclic aromatic amines (HAAs) from cooked meats. The following 10 HAAs were identified by HPLC/ESI-MS/MS: 2-amino-9H-pyrido[2,3-b]indole (2-AalphaC), 2-amino-3-methyl-9H-pyrido[2,3-b]indole (MeAalphaC), 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3-methylimidazo[4,5-f]quinoxaline (IQx), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (8-MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-DiMeIQx), 2-amino-3,7,8-trimethylimidazo[4,5-f]quinoxaline (7,8-DiMeIQx), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 2-amino-1,7,9-trimethylimidazo[4,5-g]quinoxaline (7,9-DiMeIgQx), and 2-amino-1-methylimidazo[4,5-b]quinoline (IQ[4,5-b]); the latter HAA has not previously been reported in cooked meats. The concentrations of these HAAs ranged from <0.03 to 15 ppb in cooked meats and poultry, to 75 ppb in cooked beef extract, and to 85 ppb in grill scrapings. The product ion scan mode was used to confirm the identities of these HAAs. Six other compounds were detected that appear to contain the N-methylimidazoquinoxaline skeleton on the basis of their product ion spectra, and these compounds are probable isomers of IQx, 8-MeIQx, and DiMeIQx. A number of known HAAs and novel HAAs of unknown genotoxic potential are formed at appreciable levels in cooked meats.     

Simplified extraction of ginsenosides from American ginseng (Panax quinquefolius L.) for high-performance liquid chromatography-ultraviolet analysis

Four methods were tested for extraction and recovery of six major ginsenosides (Rb1, Rb2, Rc, Rd, Re, and Rg1) found in roots of American ginseng (Panax quinquefolius): method A, sonication in 100% methanol (MeOH) at room temperature (rt); method B, sonication in 70% aqueous MeOH at rt; method C, water extraction (90 degrees C) with gentle agitation; and method D, refluxing (60 degrees C) in 100% MeOH. After 0.5-1 h, the samples were filtered and analyzed by high-performance liquid chromatography (HPLC)-UV. A second extraction by methods C and D was done, but 85-90% of ginsenosides were obtained during the first extraction. Lyophilization of extracts did not influence ginsenoside recovery. Method D resulted in the highest significant recoveries of all ginsenosides, except Rg1. Method C was the next most effective method, while method A resulted in the lowest ginsenoside recoveries. Method B led to similar recoveries as method C. All methods used one filtration step, omitted time-consuming cleanup, but maintained clear peak resolution by HPLC, and can be used for quantitative screening of ginsenosides from roots and commercial ginseng preparations.     

Persistence of soil compaction due to high axle load traffic. I. Short-term effects on the properties of clay and organic soils

Short-term effects of high axle load traffic on soil total porosity and pore size distribution were examined in field experiments on a clay (Vertic Cambisol) and an organic soil (Mollic Gleysol) for 3 years after the heavy loading. The clay soil had 48 g clay (particle size less than 2 μm) per 100 g in the topsoil and 65 g per 100 g in the subsoil. The organic soil consisted of well-decomposed sedge peat mixed with clay below 0.2 m depth down to 0.4–0.5 m and was underlain by gythia (organic soil with high clay content). The experimental traffic was applied with a tractor-trailer combination in autumn 1981. The trailer tandem axle load was 19 Mg on the clay and 16 Mg on the organic soil. There were three treatments: one pass with the heavy axle vehicle, with wheel tracks completely covering the plot area, four repeated passes in the same direction, and a control treatment without experimental traffic. During loading, the clay was nearly at field capacity below 0.1 m depth. The organic soil was wetter than field capacity.

One and four passes with the high axle load compacted both soils to a depth of 0.4–0.5 m. On the clay soil the total porosity was reduced by the heavy loading nearly as much as macroporosity (diameter over 30 μm) to 0.5 m depth. On the organic soil, macroporosity was reduced and microporosity (under 30 μm) increased in the 0.2–0.5 m layer by the heavy loading. Total porosity did not reveal the effects of compaction on the organic soil. The compaction of the clay below 0.1 m persisted for 3 years following the treatment despite annual ploughing to a depth of 0.2 m, cropping and deep cracking and freezing. Likewise, in the subsoil (below 0.2 m) of the organic soil, differences in pore size distribution persisted for a period of at least 3 years after the heavy loading.     

Persistence of soil compaction due to high axle load traffic. II. Long-term effects on the properties of fine-textured and organic soils

The long-term effects of high axle load traffic on soil structure were investigated in three field experiments. Two of the experiments were located on fine-textured mineral soils (Vertic Cambisol). The clay soil had 48 g clay (particle size less than 2 μm) per 100 g in the topsoil and 65 g per 100 g in the subsoil, and the loam soil had clay contents of 30 g and 42 g per 100 g in the topsoil and subsoil, respectively. One experiment was located on an organic soil (Mollic Gleysol) consisting of well-decomposed sedge peat mixed with clay from 0.2 to 0.4–0.5 m depth, and underlain by gythia (organic soil with high clay content). In the autumn of 1981, one pass and four repeated passes with a heavy tractor-trailer combination compacted the soils to 0.4–0.5 m depth. The trailer tandem axle load was 19 Mg on the clay and 16 Mg on the other soils.

For 9 years after the experimental traffic, the main crops grown were spring cereals. During this time, the maximum axle load applied during field operations was 5 Mg and the maximum tyre inflation pressure was 150 kPa. The clay and loam froze to 0.5 m depth for 6 and 2 years, respectively. During several growing seasons all three soils dried and cracked. In the ninth year after the loading, soil penetrometer resistance, saturated hydraulic conductivity (K_sat), macroporosity and number and area of cylindrical biopores were measured and the visual structure of the soils examined.

Compaction in the plough layer was alleviated by ploughing and natural processes, whereas in the subsoil the effects of the compaction were still measurable, in all experiments, in the ninth year after the high axle load traffic. In the clay soil in the 0.3–0.5 m layer and in the organic soil in the 0.28–0.4 m layer, the penetrometer resistance was 22–26% greater and the soil structure more massive in the plots compacted with four passes than in the control plots. In the 0.4–0.55 m layer in all soils, the loading with four passes decreased K_sat by 60–98% and macroporosity (diameter greater than 300 μm) by 37–70%. In the fine-textured mineral subsoils, cylindrical biopores were found in all treatments. The trend of the results was, however, for biopores to be fewer in compacted than in control plots.