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531.
Fraser HB Hirsh AE Steinmetz LM Scharfe C Feldman MW 《Science (New York, N.Y.)》2002,296(5568):750-752
High-throughput screens have begun to reveal the protein interaction network that underpins most cellular functions in the yeast Saccharomyces cerevisiae. How the organization of this network affects the evolution of the proteins that compose it is a fundamental question in molecular evolution. We show that the connectivity of well-conserved proteins in the network is negatively correlated with their rate of evolution. Proteins with more interactors evolve more slowly not because they are more important to the organism, but because a greater proportion of the protein is directly involved in its function. At sites important for interaction between proteins, evolutionary changes may occur largely by coevolution, in which substitutions in one protein result in selection pressure for reciprocal changes in interacting partners. We confirm one predicted outcome of this process-namely, that interacting proteins evolve at similar rates. 相似文献
532.
Throughout the northern hemisphere old forests with high abundance of dead wood are rare features in most landscapes today, and the loss of dead wood constitutes a serious threat to the existence of many species. This study, using field surveys and dendrochronology, examines the relationship between wood-inhabiting fungi and past forest utilisation along a gradient of early logging activity. Data were collected in three late-successional Scots pine forests in northern Sweden. Nonmetric Multidimensional Scaling (NMS) was then used to assess differences in species composition among the forests. Our results show that minor forest logging (22-26 cut stumps ha−1) carried out a century ago may have continuing effects on forest characteristics, including dead wood dynamics and the wood-inhabiting fungal community - especially the abundance of red-listed species. The most important effects are lower numbers of logs in early and intermediate stages of decomposition. Additionally, numbers of species (including red-listed species) can be high in forests that have been subject to low levels of logging. Overall, the high species numbers recorded in this study (n = 60-87) show that old, low-productivity pine forests harbour a considerable fraction of the total diversity of Basidiomycetes in northern Fennoscandian boreal forests. We conclude that the formation of a framework linking forest history and environmental data is vital for understanding the ecology and formulating goals for future management of these forests. 相似文献
533.
The aim of the study was to evaluate the colonizing ability and the invasive capacity of selected Campylobacter jejuni strains of importance for the epidemiology of C jejuni in Danish broiler chickens. Four C. jejuni strains were selected for experimental colonization studies in day-old and 14-day-old chickens hatched from specific pathogen free (SPF) eggs. Of the four C. jejuni strains tested, three were Penner heat-stable serotype 2, flaA type 1/1, the most common type found among broilers and human cases in Denmark. The fourth strain was Penner heat-stable serotype 19, which has been shown to be associated with the Guillain Barré Syndrome (GBS) in humans. The minimum dose for establishing colonization in the day-old chickens was approximately 2 cfu, whereas two- to threefold higher doses were required for establishing colonization in the 14-day-old chickens. Two of the C. jejuni strains were shown to be invasive in orally challenged chickens as well as in three different human epithelial cell lines. 相似文献
534.
Biobeds are used to increase the adsorption and degradation of pesticide spillage on sites used for mixing and loading and for cleaning of sprayers. The adsorption and the rate of degradation of 14C-labelled isoproturon and mecoprop (MCPP) at concentrations from 0.0005 to 25 000 mgkg(-1) were determined in biobed soil. Further leaching of the two herbicides was determined in a model biobed with a surface area of 2 m2. The biobed material showed enhanced ability to adsorb the two herbicides. Kd was 5.2 litre kg(-1) for isoproturon and 1.6 litre kg(-1) for MCPP in biobed material, which is higher than in natural soil. In different experiments with natural soil, Kd ranges from 0.07 to 0.6 litrekg(-1) for MCPP and from 1.5 to 4.6 litre kg(-1) for isoproturon in soils with varying organic carbon content. Degradation of MCPP was rapid at concentrations from 0.0005 to 500 mg kg(-1), delayed at 5000 mg kg(-1), and very slow at 25 000 mg kg(-1). For isoproturon, the relative degradation was most rapid at the lowest concentration and decreasing with increasing concentrations. After 120 days, between 55% and 8% 14C was evolved as 14CO2 at concentrations between 0.0005 and 25 000 mg kg(-1). The rate of evolution of 14CO2 indicated that degradation rates at low concentrations were of first-order and at higher concentrations of zero-order. Leaching of MCPP and isoproturon was determined in a newly established model biobed during a 2-year period. About 13% of applied MCPP and 1.4% of applied isoproturon leached out during the winter following the first autumn application (worst-case scenario). Leaching was completely prevented when the biobed had a well-developed grass cover and was covered during the winter. 相似文献
535.
Lars Krogh 《Arid Land Research and Management》2013,27(1):17-38
Soil variability has a profound impact on crop yields in low-input agriculture. The objectives of this study were to examine soil fertility variability and to identify potential soil constraints relating to farmers' conditions for agricultural production in the Sahelian zone of northern Burkina Faso. Surface soil (0-20 cm) and subsoil (20-100 cm) were sampled from 25 pedons on village-scale transects embracing three genetic soil units: two generations of sandy dune soils showing incipient development; a complex unit of clayey pediplain soils with duplex properties showing both alkaline and acid subsoil reaction; and hydromorphic, clayey valley bottom soils. Particle size distribution, pH, electrical conductivity, cation exchange capacity, exchangeable base cations and acidity, soluble base cations, organic carbon, nitrogen, total and available phosphorus, moisture constants, and bulk density were determined. Contents of organic carbon, total nitrogen, total and available phosphorus,and exchangeable potassium are low in all soils and are presumably major constraints.The sodium influenced clayey soils show high absolute variability; properties that vary are subsoil clay (5.8-38.5%), pH (5.8-10.0), cation exchange capacity (4.49-24.81 cmolc kg1), exchangeable sodium percentage (0-26), as well as electrical conductivity, structure, consistency, and available water holding capacity. The sandy soils are homogeneous and not as acid as elsewhere in the Sahel, presumably due to a dust influx. The soil fertility is generally low but highly variable, implying that constraints within production units may consist of multiple com binations of adverse chemical and physical properties, conceptually consistent with the low and variable millet yields. The variability also implies that soil character ization depends on the design of soil sampling, that average values are of doubtful use, that application of chemical fertilizers will produce very different results, and that there is no general scheme for correcting soil fertility. The application of lives tock manure will continue to be the best strategy for sustaining soil productivity. 相似文献