Bowman-Birk and Kunitz Protease Inhibitors among Antinutrients and Bioactives Modified by Germination and Hydrolysis in Brazilian Soybean Cultivar BRS 133

Soybean contains constituents that have antinutritional and bioactive properties. Enzymatic hydrolysis and germination can enhance the biological activity of these compounds in soybean. The objective of this study was to investigate the effect of germination, Alcalase (protease) hydrolysis, and their combination on the concentrations of antinutritional and bioactive compounds in Brazilian soybean cultivar BRS 133. A combination of germination and Alcalase hydrolysis resulted in the degradation of Bowman-Birk inhibitor (BBI), Kunitz trypsin inhibitor (KTI), and lunasin by 96.9, 97.8, and 38.4%. Lectin was not affected by any of the processing treatments when compared to nongerminated and nonhydrolyzed soy protein extract. Total isoflavones (ISF) and total saponins (SAP) increased by 16.2 and 28.7%, respectively, after 18 h of germination, while Alcalase hydrolysis led to the reduction of these compounds. A significant correlation was found between concentrations of BBI and KTI, BBI and lunasin, BBI and ISF, KTI and lunasin, KTI and ISF, KTI and SAP, lunasin and ISF, and ISF and SAP. Germination and Alcalase hydrolysis interacted in reducing BBI, ISF, and SAP. This study presents a process of preparing soy flour ingredients with lower concentrations of antinutritional factors and with biologically active constituents, important for the promotion of health associated with soybean consumption. In conclusion, 18 h of germination and 3 h of Alcalase hydrolysis is recommended for elimination of protease inhibitors, while bioactives are maintained by at least 50% of their original concentrations.     

Characterization of exopolysaccharides produced by Bifidobacterium longum NB667 and its cholate-resistant derivative strain IPLA B667dCo

Bifidobacteria are natural members of the human intestinal microbiota and some strains are being used as probiotics. Adaptation to bile can allow them to increase survival in gastrointestinal conditions, thus improving their viability. Bifidobacterium longum NB667 and the cholate-resistant strain B. longum IPLA B667dCo produced exopolysaccharides (EPS) that were partially characterized. Analysis by size exclusion chromatography-multiangle laser light scattering indicated that the EPS crude fractions of both strains contained two polymer peaks of different molar mass. On the basis of chromatographic techniques both peaks appeared to be heteropolysaccharides. The smaller peak was mainly composed of glucose, galactose and rhamnose whose molar ratios and linkage types showed slight variations between the EPS fractions of both strains. The bigger peak consisted of glucose and galactose; the monosaccharide composition was identical in the EPS fractions of the two microorganisms, but their infrared spectra presented some differences regarding compounds other than carbohydrates that seem to be associated to the polymer. Differences in the composition of EPS fractions did not affect the capability of crude EPS from B. longum to be fermented by the human intestinal microbiota in fecal batch cultures.     

基于信号特征分析的植物体表电信号记录模式选择

为了探索不同耦合记录模式所记录植物体表波形数据特征,选择德抗961型小麦与含羞草2类植物,分别对每株植物进行直流耦合、交流耦合同步信号记录。分析光诱导下小麦所产生的局部电位中直流耦合记录与交流耦合记录的数据差异。小麦局部电位直流耦合与交流耦合记录数据波形峰峰值差异可达25倍以上;基于短时傅里叶变换,功率密度时频分布图显示直流耦合记录到的信号局部电位的频谱主要在0.2 Hz以内。而含羞草的变异电位的上述2种耦合模式记录的波形差异相对较小,功率密度时频分布图中可以反映其信号功率谱分布在0.6 Hz以内。通过记录不同下限截止频率的数据,小麦的光诱导电信号更加清晰的反映耦合方式对信号的影响,进一步地,通过含羞草变异电位的传导特性与对小麦钙通道阻滞的对照试验来验证所测信号的真实性。因此,针对不同植物及其植物电信号本身的特点,应选择适合的耦合记录方式。     

Glyphosate and glufosinate-based herbicides: fate in soil,transfer to,and effects on land snails

Purpose  

The aim of this work was to assess the transfer and effects of two widely used herbicides on the land snail Helix aspersa during long-term exposure under laboratory conditions.     

碾后白米形变消除规律试验

为解决碾后白米抛光时碎米多的问题,该研究以碾后的白米为原料,研究温度、相对湿度以及含水率对白米形变消除时间的影响规律,采用二次旋转组合试验方法设计试验,比较了白米形变消除后抛光与碾后直接抛光的整精米率。用EXCEL软件和SAS软件处理数据,建立相关数学方程。结果表明：在试验参数范围内（温度5～45℃,含水率13%～17%,相对湿度25%～85%）,碾后白米的形变经过46～69?min可以消除;含水率、环境温度和相对湿度对形变消除时间影响显著;白米消除形变后抛光的整精米率明显高于直接抛光的整精米率。所建数学模型对碾后白米形变消除具有指导作用。     

14份小麦种质资源抗麦长管蚜遗传多样性的SSR分析

利用SSR分子标记在分子水平探讨小麦种质资源抗麦长管蚜的遗传多样性,为高效节本型和环境友好型的抗蚜育种的研究和利用提供理论依据和技术支持。结果表明：在小麦的A、B和D 3组同源染色体组上的175对SSR分子标记鉴定出了有多态性的32对引物,其中在D同源组中小麦抗蚜性的遗传多样性较高,同时在21对染色体中7D染色体上遗传...     

Foliar Nitrogen Uptake from Wet Deposition and the Relation with Leaf Wettability and Water Storage Capacity

This study assessed the foliar uptake of ¹⁵N-labelled nitrogen (N) originating from wet deposition along with leaf surface conditions, measured by wettability and water storage capacity. Foliar ¹⁵N uptake was measured on saplings of silver birch, European beech, pedunculate oak and Scots pine and the effect of nitrogen form (NH ₄ ⁺ or NO ₃ ^? ), NH ₄ ⁺ to NO ₃ ^? ratio and leaf phenology on this N uptake was assessed. Next to this, leaf wettability and water storage capacity were determined for each tree species and phenological stage, and the relationship with ¹⁵NH ₄ ⁺ and ¹⁵NO ₃ ^? uptake was examined. Uptake rates were on average five times higher (p?<?0.05) for NH ₄ ⁺ than for NO ₃ ^? and four times higher for deciduous species than for Scots pine. Developing leaves showed lower uptake than fully developed and senescent leaves, but this effect was tree species dependent. The applied NH ₄ ⁺ to NO ₃ ^? ratio did only affect the amount of N uptake by senescent leaves. The negative correlation between measured leaf contact angles and foliar N uptake demonstrates that the observed effects of tree species and phenological stage are related to differences in leaf wettability and not to water storage capacity.     

电动多旋翼植保无人机升力特性综合测评方法

升力特性是电动多旋翼植保无人机性能测试的重要参数之一。为了实现对电动多旋翼植保无人机升力特性的性能检测,针对不同型号、不同规格的电动多旋翼植保无人机在评价过程中存在无统一的评价指标问题,该文提出了一种半系留式电动多旋翼植保无人机升力特性的测试与评价方法,包括性能检测平台、升力特性测试方法及指标、升力特性的评价方法。为了验证方法的可行性,对3种不同机型(分别为四旋翼机型Ⅰ、六旋翼机型Ⅱ、八旋翼机型Ⅲ)进行了升力特性指标的性能测试试验。试验结果表明:3种机型在功率载荷、重量效率、热效比等方面有较大差异,功率载荷最好的机型Ⅲ比最差的机型Ⅰ大7.6 m N/W,重量效率最好的机型Ⅰ比最差的机型Ⅱ大0.33,热效比最好的机型Ⅲ比最差的机型Ⅱ大10.5 N/℃,反映出3种机型在设计过程中整个动力系统效率、机型整体结构和材料选择上的差异,从而在整机作业性能上表现出差异。在上述指标测试的基础上,结合无人机动力系统数学模型,提出了运用功率载荷、重量效率和热效比进行电动多旋翼植保无人机升力特性综合评价的评分方法,对上述3种机型进行综合评分的结果为:机型Ⅲ机型Ⅰ机型Ⅱ,该结果表明所提出的评价方法能有效对不同类型电动多旋翼植保无人机的升力特性进行综合评判。该文所给出的测试与评价方法,不仅能用于电动多旋翼植保无人机性能的评测,还能为机型性能的进一步改进提供参考。