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361.
S. Massart Y. Brostaux L. Barbarossa V. César M. Cieslinska O. Dutrecq F. Fonseca R. Guillem A. Laviña A. Olmos S. Steyer T. Wetzel J. Kummert M. H. Jijakli 《European journal of plant pathology / European Foundation for Plant Pathology》2008,122(4):539-547
The operational capacity of a duplex RT-PCR method for simultaneous detection of Prune dwarf virus (PDV) and Prunus necrotic ringspot virus (PNRSV) has been established by nine European laboratories. A total of 576 samples from Prunus trees with known sanitary status, corresponding to 32 samples in two repetitions for each laboratory, were analysed. The
level of sensitivity achieved by the method was 98.3% for PDV and 90.4% for PNRSV. The specificity was 87.4% for PDV and 94.3%
for PNRSV. The unilateral 95% confidence intervals were calculated for all these values. Cohen’s Kappa coefficient of repeatability
and reproducibility of the technique indicated a strong agreement between data. Likelihood ratios were 7.50 (positive) and
0.02 (negative) for PDV. For PNRSV, the positive likelihood ratio was 15.00 while the negative likelihood ratio was 0.11.
In addition, post-test probabilities of infection were calculated to manage the risk associated with the routine use of this
method. This allows an accurate test result interpretation to facilitate the integration of this new technique into a certification
scheme. 相似文献
362.
Resin flow is the primary means of natural defense against southern pine beetle (Dendroctonus frontalis Zimm.), the most important insect pest of Pinus spp. in the southern United States. As a result, factors affecting resin flow are of interest to researchers and forest managers. We examined the influence of fertilization, artificial wounding and fungal inoculation on resin flow in 6- and 12-year-old stands of loblolly pine (Pinus taeda L.) and determined the extent of that influence within and above the wounded stem area and through time. Fertilization increased constitutive resin flow, but only the younger trees sustained increased resin flow after wounding and inoculation treatments. An induced resin flow response occurred between 1 and 30 days after wounding and inoculation treatments. Wounding with inoculation resulted in greater resin flow than wounding alone, but increasing amounts of inoculum did not increase resin flow. Increased resin flow (relative to controls) lasted for at least 90 days after wounding and inoculation. This increase appeared to be limited to the area of treatment, at least in younger trees. The long-lasting effects of fungal inoculation on resin flow, as well as the response to fertilization, suggest that acquired resistance through induced resin flow aids in decreasing susceptibility of loblolly pine to southern pine beetle. 相似文献
363.
Franca CS Menezes FS Costa LC Niculau ES Alves PB Pinto JE Marçal RM 《Fitoterapia》2008,79(7-8):569-573
Ocimum selloi essential oil (2, 20, and 200 mg/kg; p.o.) reduced, in a dose-dependent way, the abdominal contraction induced by acetic acid (0.6%; i.p.) and the diarrhea episodes induced by castor oil in mice. At the higher dose (200 mg/kg; p.o.), the essential oil significantly reduced intestinal transit (P<0.05) in the charcoal meal test. The main component detected in O. selloi essential oil was methyl chavicol (98%; GC and GC/MS).These effects seems to support the use of O. selloi against diarrhea, intestinal spasm and visceral pain. 相似文献
364.
José M. Vidal Alexis Fonseca Pamela Ruiz Daniela Sepúlveda Carlos Carrasco Stefano Scilipoti Javier Barros Ariel Valenzuela Ricardo Saavedra Nathaly Ruiz-Tagle Homero Urrutia 《Journal of fish diseases》2023,46(1):1-15
This study evaluated the probiotic potential of the biofilm formed by the strain Pseudomonas sp. RGM2144 on rainbow trout survival. When challenged with the fish pathogen Flavobacterium psychrophilum, Pseudomonas sp. RGM2144 increased rainbow trout survival to 92.7 ± 1.2% (control: 35.3 ± 9.5%, p < .0001). The draft genome of Pseudomonas sp. RGM2144 is 6.8 Mbp long, with a completeness 100% and a contamination of 0.4%. The genome contains 6122 protein-coding genes of which 3564 (~60%) have known functions. The genome and phylogeny indicate that Pseudomonas sp. RGM2144 is a new species in the Pseudomonas genus, with few virulence factors, plasmids, and genes associated with antimicrobial resistance, suggesting a non-pathogenic bacterium with protective potential. In addition, the genome encodes for 11 secondary metabolite biosynthetic gene clusters that could be involved in the inhibition of F. psychrophilum. We suggest that Pseudomonas sp. RGM2144 may be applied as a probiotic in salmonid fish farming. 相似文献
365.
366.
Marcus Vinicius Vieira Borges Janielle de Oliveira Garcia Tays Silva Batista Alexsandra Nogueira Martins Silva Fabio Henrique Rojo Baio Carlos Ant?nio da Silva Junior Gileno Brito de Azevedo Glauce Taís de Oliveira Sousa Azevedo Larissa Pereira Ribeiro Teodoro Paulo Eduardo Teodoro 《林业研究》2022,(2):591-599
In forest modeling to estimate the volume of wood, artificial intelligence has been shown to be quite effi-cient, especially using artificial neural networks (A... 相似文献
367.
José Marcelo Soriano Viana Ísis Fernanda de Almeida Marcos Deon Vilela de Resende Vinícius Ribeiro Faria Fabyano Fonseca e Silva 《Euphytica》2010,174(1):31-39
Best Linear Unbiased Prediction (BLUP) has become the most widely used method for genetic assessment of animal and perennial species, and it is potentially relevant for annual crops. The objective of this study was to assess this method for selection within non-inbred families in recurrent breeding programs. The ‘animal model’ was fitted. The data were expansion volume (EV) and grain yield of plants in recombination plots of two to three selection cycles in the popcorn population Viçosa, with half- and full-sib progenies. The ASReml program was used to perform the analyses. For both EV and yield the breeding values predicted from the additive and additive-dominant models were highly correlated. Multi-generation BLUP was, in general, more accurate than single-generation analysis. These two methods resulted in highly correlated predicted breeding values. The dominance genetic values predicted from the single- and multi-generation analysis were also highly correlated. The pedigree information reduced the percentage of coincidences among the selected individuals relative to phenotypic selection mainly in the population structured in half-sib families. Based on breeding values predicted by BLUP analysis, the most efficient selection procedure was mass selection. 相似文献
368.
Optimizing initial feeding of the Pike silverside Chirostoma estor: oil droplet depletion,point of no return,growth and fatty acid utilization in larvae fed enriched rotifers 下载免费PDF全文
M.G. Ríos‐Durán P. Navarrete‐Ramírez J. Fonseca‐Madrigal E. Mendez‐Ortiz E. Palacios I.S. Racotta C.C. Martínez‐Chávez L.G. Ross C.A. Martínez‐Palacios 《Aquaculture Nutrition》2016,22(3):517-526
The point of no return (PNR) and disappearance of the oil droplet were measured in Chirostoma estor larvae as a function of the time of first feeding. In a separate trial, growth and survival of larvae fed rotifers enriched with Chlorella sp., cod liver oil and corn oil were assessed. Fatty acid and lipid composition of eggs, oil droplets, egg yolk, feed and larvae were also evaluated. The PNR was found between 7 and 8 days posthatching (dph). Total oil droplet depletion occurred between 7 and 11 dph, depending on the time of first feeding. Best growth and survival were obtained in larvae fed with Chlorella‐enriched rotifers, followed by those fed cod liver oil‐enriched rotifers. In larvae fed corn oil, Chlorella and cod liver oil‐enriched rotifers, total oil droplet depletion took place on days 9, 10 and 11, respectively. There was a direct relationship between presence and duration of oil droplets and the survival of larvae under different starvation conditions. The feed source could prolong the existence of the oil droplet depending on particular dietary supply of essential fatty acids; the time of its disappearance could be a useful indicator of larval vigour and health status. 相似文献
369.
370.
Bianca G. Ambrogi Marcy G. Fonseca Miryan D. A. Coracini Paulo H. G. Zarbin 《Journal of pest science》2009,82(1):55-60
The calling behaviour of virgin females Condylorrhiza
vestigialis Guenée, 1854 (Lepidoptera: Crambidae) and the female pheromone gland extract activity were studied under laboratory conditions.
Most of the females started calling from their first scotophase period after emergence. Maximum calling occurred between the
seventh and tenth hours of the scotophase period. The length of the calling increased with age until the fourth scotophase,
but the onset of calling time did not differ with age. The number of calling bouts increased significantly with age, but the
mean duration of each calling bout (20.8 min) did not vary with age. Extracts of pheromone glands evaluated in a Y-tube olfactometer
attracted significantly more males than control, 70 and 30%, respectively. Gas chromatographic–electroantennogram detection
(GC–EAD) analysis of these extracts indicated the presence of a single EAD-active peak, the putative sex pheromone of the
species. This pheromone compound may be suitable for monitoring populations densities of C. vestigialis, and for detection of the onset of the seasonal flight period. An efficient pheromone is of importance also with respect
to current attempts to develop new control methods for this important pest of Populus spp. in Brazil. 相似文献