Helminthiasis characterization and anthelmintic efficacy for ewes and lambs raised in tropical semiarid region

In this study the helminthiasis and anthelmintic effectiveness in ewes and lambs were evaluated in a semiarid region of Brazil. Twelve sheep farms were investigated using semi-structured questionnaires and fecal egg count (FEC) reduction test was employed to analyze the profile of anthelmintic resistance. Groups of at least 10 animals with FEC ≥ 300 were selected. After 12 h of fasting, homogeneous groups of lambs or ewes were treated with albendazole, levamisole moxidectin, or oxfendazole and control groups were not treated. Feces were collected before treatments and 14 days after, and larvae genera were identified after cuprocultures in both periods. Extensive grazing was the predominant creation system, using hybrid Santa Ines animals. The separation by age was promoted in 75% of herds; however, maternity pickets there were only in three farms. The strategic treatments were performed only in 8.4% of sheep farms and 16.6% used the anthelmintic efficacy test and alternated anthelmintic classes after 1 year. The initial FEC means for lambs were significantly higher than ewe FEC averages. For lamb tests, moxidectin and levamisole showed higher efficacy (p ≤ 0.05) than benzimidazoles. For ewe tests, moxidectin and levamisole showed efficiencies >75%. Haemonchus spp. and Trichostrongylus spp. were the most frequent nematodes before treatments and the genus Haemonchus was the most prevalent after anthelmintic treatments (p < 0.05). Variations of anthelmintic susceptibility were observed for categories and herds evaluated, which emphasizes the importance of the effectiveness tests for the choice of anthelmintics for ewes and lambs.     

Soil loss and run-off in young forest stands as affected by site preparation technique: a study in NE Portugal

Soil loss rates currently recorded in forests are very low. Nevertheless, that may not be the case during stand installation and early tree growth stage, when soil is disturbed and scarcely covered. Site preparation techniques, performed to improve soil conditions for plant growth, should help reducing this erosion potential. In this study, several site preparation techniques were applied prior to installing a mixed stand (Pseudotsuga mensiezii and Castanea sativa) and a subsequent monitoring scheme of run-off and soil loss ran for 2?years in order to compare their effectiveness for erosion control. The experimental area, near Macedo de Cavaleiros, NE Portugal, at 700?m elevation, with annual means of 656?mm rainfall and 12°C temperature, has Mediterranean climatic conditions. Experimental design comprised three blocks, corresponding to different topographical positions (near flat plateau, moderate slope shoulder and steep mid-slope), where eight treatments were randomly distributed in plots with 375?m² area: (1) Original soil control (no intervention on the original abandoned field); (2) No subsoiling, no ploughing, plantation with hole digger; (3) Subsoiling over the whole area, with covering shovel; (4) No subsoiling, contour bunds shaped by two plough passes; (5) Subsoiling in future plantation rows, contour bunds shaped by two plough passes; (6) Subsoiling over the whole area, contour bunds shaped by two plough passes; (7) Subsoiling over the whole area, contour ploughing over the whole area; and (8) Potential erosion (subsoiling over the whole area, ploughing downhill). Sediment and water exported from small plots (2.5?m² average area), two replicates per treatment and block, were collected after each rainfall erosion event, in a total of 21, summing 1,876-mm precipitation in 2?years. Mean annual run-off and soil loss in the original soil were 3.4?mm and 11.6?g?m^?2, respectively. In treatments 2–7, values were higher 3–7 times, for run-off, and 5–12 times, for soil loss. Potential erosion averages 2.3 t ha^?1 year^?1. Soil loss and run-off tend to increase with tillage intensity associated with site preparation technique, even though average two-year losses, in all cases, are below tolerable rates. Soil loss and run-off rates decreased with time, becoming globally negligible after 2?years. Slight and moderate soil disturbance intensity site preparation techniques reduce erosion rates to 30% of potential erosion, halving the critical period when above tolerance rates may occur.     

Technical note: stearidonic acid metabolism by mixed ruminal microorganisms in vitro

Dietary supplementation of stearidonic acid (SDA; 18:4n-3) has been considered a possible strategy to increase n-3 unsaturated fatty acid content in ruminant products; however, little is known about its metabolism in the rumen. In vitro batch incubations were carried out with bovine ruminal digesta to investigate the metabolism of SDA and its biohydrogenation products. Incubation mixtures (4.5 mL) that contained 0 (control), 0.25, 0.50, 0.75, 1.00, 1.25, or 1.50 mg of SDA supplemented to 33 mg (DM basis) of commercial total mixed ration based on corn silage, for dairy cows, were incubated for 72 h at 39°C. The content of most fatty acids in whole freeze-dried cultures was affected by SDA supplementation. Branched-chain fatty acids decreased linearly (P < 0.01), and odd-chain fatty acids decreased quadratically (P < 0.01), particularly from 1.00 mg of SDA and above, whereas most C18 fatty acids increased linearly or quadratically (P ≤ 0.04). Stearidonic acid concentrations at 72 h of incubation were very small (<0.6% of total fatty acids and ≤0.9% of added SDA) in all treatments. The apparent biohydrogenation of SDA was extensive, but it was not affected by SDA concentration (P > 0.05). Biohydrogenation followed a pattern similar to that of other C18 unsaturated fatty acids up to 1.00 mg of SDA. Stearic acid (18:0) and vaccenic acid (18:1 trans-11) were the major fatty acids formed, with the latter increasing 9-fold in the 1.00 mg of SDA treatment. At greater inclusion rates, 18:0 and 18:1 trans isomers decreased (P ≤ 0.03), accompanied by increases in unidentified 18:3 and 18:4 isomers (P = 0.02), suggesting that the biohydrogenation pathway was inhibited. The present results clearly indicate that SDA was metabolized extensively, with numerous 18:4 and 18:3 products formed en route to further conversion to 18:2, 18:1 isomers, and 18:0.     

Use of Tannic Acid as Dough Oxidizing and Vitamin C Protective Agent

Trials with tannic acid in three concentrations (0.1, 0.2, and 0.3%) in a wheat flour dough were run to test its property to preserve the ascorbic acid degradation during baking and its performance in the dough viscoelasticity measured by its extensigraphic properties. The addition of tannic acid to the dough in the cited concentrations increased its resistance to extension (RE) and consequently reduced its extensibility (E) in the same way that ascorbic acid performed but using concentrations 10× smaller. In a dough containing ascorbic acid 0.02% and tannic acid 0.3%, the ascorbic acid retention after 10 days of storage was 34.8%, which represents 154% of the recommended daily intake of vitamin C by FAO/WHO (2002) for an adult (19–65 years old). The addition of tannic acid to the dough also increased the bread specific volume.     

Social behaviour of buffalo heifers during the establishment of a dominance hierarchy

This study investigated the social behaviour of buffalo heifers during the establishment of a dominance hierarchy under conditions that differed in space availability (pen, small pasture and large pasture), food competition and in the presence or absence of an intact bull. The herd consisted of 21 Mediterranean–Murrah crossbreed buffalo heifers and one bull. During the establishment of dominance hierarchy, one observer recorded all of agonistic interactions occurring in the pen, in the small pasture and in the large pasture. For the food competition test, the females were first separated into groups (G) of seven animals and the behavioural interactions within these groups were observed. The group levels were determined by the sociometric parameters (h′: Landau linearity index and DCI: directional consistency index). The percentage of unknown, one-way and two-way dyadic relationships; a social index, hierarchical position and correlations among feeding duration, latency, social index and hierarchical position were calculated. In the large pasture, the DCI value was 55.24% of the dyadic relationships were one-way and 36.19% were unknown; whereas in the small pasture and pen most of the dyads were unknown (54–63%) or one-way (33-41%). The social indexes observed were low (0.004 and 0.122). In the food competition test, h′ values were not significant for groups G1, G2, G3 and G6, and significant for Groups G4 and G5. The percentages of two-way dyadic relationships were high in G4 (80.95%) and G6 (100%) groups. Correlations among feeding duration and latency, social position and social index were all negative and significant. There were no significant correlations between body weight and social index or hierarchy position. This study showed that buffalo heifers established social dominance relationships among several of the herd members, but because there were several unknown dyads, the hierarchy is classified as semi-linear hierarchy. Two factors seemed to affect the establishment of social dominance and a hierarchy: adjusting to a new environment and the presence of an adult intact male, and the dominant females had advantage over the subordinate females during feeding in a trough. To reduce agonistic encounters between buffalo heifers suggest provide more space in the trough during feeding.     

Acetate and sulphate as regulators of potential methane production in a tropical coastal lagoon

Journal of Soils and Sediments - We evaluated the influence of acetate and sulphate addition on methanogenesis in sediment layers of cores of different carbon and sulphate concentrations from the...     

Apparent digestibility coefficients of European grain legumes in rainbow trout (Oncorhynchus mykiss) and Nile tilapia (Oreochromis niloticus)

Two trials were carried out to evaluate the apparent digestibility coefficients (ADCs) of dry matter (DM), crude protein (CP), lipids, starch and gross energy (GE) of six European varieties of grain legumes, namely chickpea‐type Kabuli (CHK), chickpea‐type Desi (CHD), field pea (FP), faba bean (FB), white lupin (WL) and chickling vetch (CV), in rainbow trout and Nile tilapia juveniles. The ADCs were measured using a reference diet and six experimental diets (700 g/kg of the reference diet and 300 g/kg of each raw grain legume) containing 10 g/kg chromic oxide as inert marker. Additionally, grain legumes were analysed for the organic acids profile. In rainbow trout, FP presented the highest ADCs of DM, CP and GE, whereas chickpeas and FB had the lowest DM, GE and starch ADC values. In Nile tilapia, the lowest values of nutrients (except starch) and energy ADCs were found for FP and CV. Nutrients and energy of chickpeas, WL and FB were better digested by tilapia, whereas FP was better digested by trout. Overall results reveal raw grain legumes as promising feed sources for both fish species.     

Molecular and phylogenetic characterization of Theileria spp. parasites in autochthonous bovines (Mirandesa breed) in Portugal

A survey was conducted during the months of April-June 2003 in the northeast Portugal (Bragan?a district) in order to characterize the hemoparasite population of an autochthonous Mirandesa breed of Bos taurus. The polymerase chain reaction (PCR) analysis of the bovine blood revealed that 3 out of 116 animals were infected with Theileria and/or Babesia parasites, while reverse line blot hybridisation (RLB) analysis showed that these animals were infected with Theileria buffeli/orientalis. Cloning and sequencing confirmed the RLB results. Database sequence searches combined with phylogenetic analysis of the partial 18S ribosomal RNA gene sequences obtained enabled us to place the parasites in question as members of the T. buffeli/orientalis group, confirming the PCR/RLB diagnosis.