5-Hydroxy-3,6,7,8,3',4'-hexamethoxyflavone induces apoptosis through reactive oxygen species production, growth arrest and DNA damage-inducible gene 153 expression, and caspase activation in human leukemia cells

This study examined the growth inhibitory effects of structurally related polymethoxylated flavones in human cancer cells. Here, we report that 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-OH-HxMF) induces growth inhibition of human cancer cells and induction of apoptosis in HL-60 cells through modulation of mitochondrial functions regulated by reactive oxygen species (ROS). ROS generation occurs in the early stages of 5-OH-HxMF-induced apoptosis, preceding cytochrome c release, caspase activation, and DNA fragmentation. The changes occurred after single breaks in DNA were detected, suggesting that 5-OH-HxMF induced irreparable DNA damage, which in turn triggered the process of apoptosis. Up-regulation of Bax was found in 5-OH-HxMF-treated HL-60 cells. In addition, a caspase-independent pathway indicated by endonuclease G also contributed to apoptosis caused by 5-OH-HxMF. Antioxidants suppress 5-OH-HxMF-induced apoptosis. 5-OH-HxMF markedly enhanced growth arrest DNA damage-inducible gene 153 (GADD153) protein in a time-dependent manner. N-acetylcysteine (NAC) and catalase prevented up-regulation of GADD153 expression caused by 5-OH-HxMF. These findings suggest that 5-OH-HxMF creates an oxidative cellular environment that induces DNA damage and GADD153 gene activation, which in turn helps trigger apoptosis in HL-60 cells. Meanwhile, ROS were proven an important inducer in this apoptotic process. The C-5 hydroxyl on the ring of 5-OH-HxMF was found to be essential for the antiproliferative and apoptosis-inducing activity. Our study identified the novel mechanisms of 5-OH-HxMF-induced apoptosis and indicated that these results have significant applications as potential chemopreventive and chemotherapeutic agents.     

茶产业巩固脱贫攻坚成果的实践探索——以三江侗族自治县为例

发展茶产业可带动地区提质增效和增加农户收益,为乡村振兴提供有力支撑.三江侗族自治县利用生态优势,加强标准化基地建设,培育荼产业龙头企业,出台激励政策,促进产品销售,创建核心品牌,加强人才培养等推进荼产业高质量发展,取得了一定成效.三江侗族自治县发展茶产业主要经验包括加强组织领导、加强资金保障、引领绿色发展、提高产业品质、完善配套设施.为进一步促进三江侗族自治县茶产业高质量发展,全面推进乡村振兴,应提升产业品质、打造区域公用品牌、加强一二三产融合发展.     

Immune responses of orange-spotted grouper,Epinephelus coioides,against virus-like particles of betanodavirus produced in Escherichia coli

Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a serious disease of cultured marine fish worldwide. Virus-like particles (VLPs) are one of the good novel vaccine candidates to control this disease. Until now, betanodavirus vaccine studies mainly focused on the humoral immune response and mortality after virus challenge. However, little is known about the activation of genes responsible for cellular and innate immunity by vaccines. In the present study, VLPs of orange-spotted grouper nervous necrosis virus (OGNNV) were produced in prokaryotes and their ability to enter Asian sea bass cells was the same as native virus, suggesting that they possess a similar structure to OGNNV. VLPs immunogenicity was then determined by intramuscularly vaccinating Epinephelus coioides at different concentrations (1.5 or 15 μg g^?1 fish body weight, FBW) and immunizing frequencies (administration once, twice and thrice). A single vaccination with the dosage of 1.5 μg g^?1 FBW is enough to provoke high titer antibodies (average 3 fold higher than that of negative control) with strong neutralizing antibody titer as early as 1 week post immunization. Furthermore, quantitative PCR analysis revealed that eleven genes associated with humoral, cellular and innate immunities were up-regulated in the liver, spleen and head kidney at 12 h post immunization, correlating with the early antibody response. In conclusion, we demonstrated that VLP vaccination induced humoral immune responses and activated genes associated with cellular and innate immunity against betanodavirus infection in orange-spotted grouper.     

文心兰切花栽培技术和经验浅谈

从文心兰生长特点、生活环境、品种选择出发,结合海南地区实际情况,对文心兰切花栽培技术进行综述,重点总结了文心兰切花生产中的栽培基质、水肥管理、病虫害防治技术,并对如何提高文心兰切花产量提出可行性对策。     

中国对虾往西北内陆咸水水域移植的生产性试养研究

本文介绍了通过采用引进河水、压碱降盐等改良水质的技术措施,使中国对虾首次成功地移植到内陆咸水水域。经过1个月的试养,中国对虾平均体长增长3.82 cm,平均体重为1.82 g。     

Water-Soluble Polysaccharides Extracted from Pueraria lobata Delay Aging of Caenorhabditis elegans under Heat Stress

Plant Foods for Human Nutrition - Pueraria lobata is a perennial legume, commonly used as a food source in China. The polysaccharides extracted from P. lobata have demonstrated various biological...     

Ciguatoxin in moray eels raising the risk for seafood safety in Viet Nam

Fisheries Science - Recently, suspected ciguatera fish poisoning (CFP) cases caused by the consumption of moray eels, popular seafood for locals and tourists, have been reported in Viet Nam....     

甜菜链式纸钵苗分离装置参数优化

对甜菜链式纸钵苗自动移栽机的链式纸钵苗分离装置主要参数进行了优化试验研究。介绍了分离装置的结构及工作原理;采用二次正交旋转中心组合优化试验方法,以分离率、钵苗损伤率为目标函数,以钵苗引出速度、分离轮与引出轮速比、分离轮倾斜角度为影响因子,对影响分离装置性能的工作和结构参数组合进行了优化试验研究。结果表明：当引出速度78～93 mm/s、速比6～7、分离轮倾斜角度为13°时,分离率达95%以上,钵苗损伤率小于6%。为甜菜链式纸钵苗分离装置的设计提供了依据。