红壤旱地芝麻防早衰叶面调控剂筛选研究

研究结果表明,速效肥料、生长调节剂及抗病药剂对保持芝麻生长中后期的正常生长都能起到积极作用。"磷酸二氢钾+硝酸铵+腐植酸+赤霉素"配制能有效防控红壤旱地芝麻早衰,在此配方基础上,添加代森锰锌能对防控芝麻叶部及茎部病害起到一定的作用,从而增强了红壤旱地上芝麻的抗早衰能力。     

农业物联网在新疆棉花产业中的应用与发展——以农八师一五〇团为例

为解决新疆农业物联网综合利用程度不高的问题,文中介绍了目前农业物联网中关键技术研究进展,同时以新疆农八师一五O团为例,对其棉花产业对农业物联网的应用进行了分析,并针对农业物联网在新疆的应用过程出现的问题进行了分析,提出了相应的解决策略。最后对一五O团的棉花产业的发展进行了总结。     

沼液追肥对制种玉米产量与土壤化学性质的影响

研究沼液作追肥对制种玉米产量及土壤化学性质的影响。文章以制种玉米为试验材料,采用计随机区组试验设,研究沼液追肥对制种玉米经济性状、产量、土壤化学性质、经济效益的影响。结果表明,T1(沼液1500kg·hm-2+142.50 kg N·hm-2)有机质含量最高,与其他处理间达到了显著差异;T1的土壤速效钾含量最高,达12.67 g·kg-1,与CK(150 kg N·hm-2),T2(沼液22500 kg·hm-2+138.75 kg N·hm-2)间达到了显著差异;土壤速效磷含量,土壤p H值,土壤电导率差异不显著;不同处理对穗行数,行粒数、穗粒重的影响差异不显著,T3(沼液30000 kg·hm-2+135 kg N·hm-2)千粒重最高,与CK,T2,T4(沼液37500 kg·hm-2+131.25 kg N·hm-2)间差异达到了显著水平;T2的产量,产值,经济效益最高。沼液做追肥可提高产量,降低生产成本,但必须和化肥配合施用。     

Prokaryotic Expression and Antigenicity Analysis of Porcine Japenese Encephalitis Virus Envelope Protein Domain Ⅲ

In order to analyze the antigenicity of porcine Japanese encephalitis virus (JEV) E protein domain Ⅲ, which was expressed by pET-28a vector with His-tag and purified through Ni-NTA, the BALB/c mice were immunized with the purified protein.We identified the antigenicity of domain Ⅲ of E protein and the anti-mice and anti-porcine JEV E Ⅲ protein specific antibody titers by SDS-PAGE, Western blotting, indirect ELISA and IFA.SDS-PAGE results showed the expressed target protein existed mainly in the form of inclusion body.Western blotting, ELISA test results showed that the protein had good reactivity with anti-serum.The mice immunized with the purified JEV E Ⅲ protein generated 1×10⁵ anti-JEV E Ⅲ protein specific antibody titers by ELISA, and the porcine immunized with the porcine JEV generated 5.1×10⁴ anti-JEV specific antibody titers.The IFA results showed that JEV E Ⅲ protein anti-serum could identify JEV antigen.The above results showed that the recombinant JEV E Ⅲ had good antigenicity.These results provided important basis for development of diagnostic antigen for JEV.     

Screening of Probiotics Inhibiting E. coli K88 in vitro

The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 (IPEC-1).The activity of lactate dehydrogenase (LDH) in the supernatant was measured after incubating for 2.5 h.At the same time, the probiotics and E.coli K88 were co-cultured in vitro, the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lactobacillus casei and Bacillus coagulans could significantly reduce LDH activity (P<0.05), decrease the damage of E.coli K88; Bacillus coagulans could inhibit the growth of E.coli K88.At the same time, Bacillus coagulans could resist high temperature, acid and bile salt.The results showed that Bacillus coagulans strains had great potential as the application of probiotics strains.The methods could be used as a model of screen probiotics which could inhibit the growth of E.coli K88 in vitro.     

氮肥配施生物炭和脲酶抑制剂对夏玉米-冬小麦产量及氮素利用的影响

于2019—2021年采用再裂区设计,设置氮肥、生物炭和脲酶抑制剂3个因素,主处理设5个氮水平：0、75、150、225 kg·hm^-2和300 kg·hm^-2,副处理设2个生物炭水平：0 t·hm^-2和7.5 t·hm^-2,副副处理设2个脲酶抑制剂水平：0%和2%,共20个处理,研究氮肥配施生物炭和脲酶抑制剂对夏玉米-冬小麦轮作体系作物产量和氮肥吸收利用的影响。结果表明,施用生物炭显著提高夏玉米和冬小麦产量、植株氮素吸收量、氮肥表观利用率、氮素收获指数以及夏玉米地上部生物量,较不施生物炭处理分别增加4.4%和2.9%、2.3%和3.0%、25.8%和13.5%、4.9%和6.1%、4.5%;氮肥单独配施生物炭可显著提高夏玉米和冬小麦产量、植株氮素吸收量和氮肥表观利用率,且氮肥和生物炭具有显著的交互效应。施用脲酶抑制剂显著增加夏玉米植株氮素吸收量和氮肥表观利用率,较不施脲酶抑制剂处理分别提高1.5%和3.0%;氮肥单独配施脲酶抑制剂可提高夏玉米植株氮素吸收量和氮肥表观利用率,但氮肥与脲酶抑制剂无显著...     

自吸泵研究现状及发展趋势

自吸泵广泛用于喷灌、消防等场合。全面总结和分析了自吸泵的发展史，归纳了自吸泵自吸时间和最大自吸高度的计算模型，总结了自吸性能影响因素研究现状，介绍了回流孔大小、位置的计算公式，以及隔舌间隙及泵内储水容积和气液分离室容积的控制；阐明了轴封的气密性、吸入管路的气密性、浸入液面下的管路深度和入口管路的容积这些外部因素对自吸性能的影响机理；指出了自吸泵进一步发展的趋势和方向，为后续研究工作提供了指导性建议。     

我国畜禽舍机械式降温系统的研究与发展现状

论述了我国畜禽舍机械式降温系统的研究与应用现状;比较分析了各种降温系统的优缺点与应用条件;考虑到设备投资与应用成本,认为采用正压送风的湿帘冷风机具有较大的应用潜力,集中式雾化降温系统和喷雾冷风机具有一定的优势;同时,指出了喷雾降温系统的研究重点是开发低成本的设备和完善适用于低压喷雾的集中式雾化降温系统的水雾分离装置.     

初析农业信息资源的整合及模式

随着农业信息化的推进,农业信息网站迅速发展起来。但是问题突出,主要是各农业网站以及各级政府涉农部门的信息是一个个信息孤岛,信息资源无法共享且重复建设,信息的有效性和时效性差。为此,以黑龙江省农业信息化建设为背景,提出了加强农业信息资源整合的必要性及其模式,从而推进农业信息化“最后一公里”的建设。