低温下增施CO2对辣椒产量及品质的影响

以方形椒“曼迪”为试材,研究日光温室环境中低温下增施CO₂对辣椒形态、叶绿素含量、核酮糖-1,5-二磷酸羧化酶活性、净光合速率及辣椒果实产量和品质的影响,以期为设施农业中辣椒产量和品质提供参考依据。结果表明：1)单独增施CO₂,辣椒长势最好,形态指标、光合参数、果实大小、辣椒单果质量及总产量均达到最大。2)单独低温处理,辣椒长势最差,形态指标、光合参数、果实大小、产量及品质显著降低。3)与单独低温处理相比,低温下增施CO₂处理明显改善辣椒长势,辣椒果实大小、产量及品质均有显著提升。低温下增施CO₂处理可缓解低温对辣椒的生长胁迫,有利于辣椒生长,增加辣椒产量,提高辣椒品质。     

果蔬型甜玉米新品种京科甜191的选育

京科甜191是以超甜玉米自交系T68为母本,自交系T6302为父本,通过杂交选育而成的果蔬型甜玉米品种。果穗筒型,穗长19.0 cm,穗粗5.3 cm,穗行数16~18行,行粒数36~38粒,粒行整齐。籽粒亮黄色,外观漂亮。平均每667 m2鲜果穗产量900 kg左右,出籽率约67.4%。鲜籽粒含粗蛋白2.98%,粗脂肪1.37%,总糖6.7%,口感甜脆清爽,皮薄无渣。播种至采收平均85.4 d(天),适宜京津冀及类似生态区种植。     

不同抗性玉米品种接种甘蔗花叶病毒(SCMV)后4种防御酶活性变化研究

 植物在长期进化过程中,不断抵御外部伤害,包括有害生物的入侵,其体内逐渐形成了一套完整的保护机制。     

Organogenesis and foetal haemodynamics during the normal gestation of healthy black‐rumped agoutis (Dasyprocta prymnolopha,Wagler, 1831) bred in captivity

The objective of this study was to define the patterns of organogenesis and foetal haemodynamics during the normal gestation of healthy agoutis (Dasyprocta prymnolopha) kept in captivity. Thirty pregnant agoutis that ranged in size from small to medium and weighed between 2.5 and 3 kg underwent B‐mode and Doppler ultrasonography for the biometric evaluation of the foetal organs. The foetal aortic blood flow proved to be predominantly systolic, and the measured flow velocity was 78.89 ± 2.95 cm/s, with a maximum pressure gradient of 2.12 ± 0.27 mmHg. The liver was characterized by its large volume, occupying the entire cranial aspect of the abdominal cavity, and it was associated cranially with the diaphragm and caudally with the stomach. The flow velocity in the portal vein was estimated to equal 12.17 ± 2.37 cm/s, with a resistivity index of 0.82 ± 0.05. The gallbladder was centrally located and protruded cranially towards the diaphragm. The spleen was visualized as an elongated structure with tapered cranial and caudal extremities, and the foetal kidneys were visualized bilaterally in the retroperitoneal region, with the right kidney positioned slightly more cranially than the left. The morphological characterization and hemodynamic analysis of the foetal organs of black‐rumped agoutis via B‐mode and Doppler ultrasonography allow determination of the vascular network and of reference values for the blood flow required for perfusing the anatomical elements essential for maintaining the viability of foetuses at different gestational ages.     

不同品种鸡蛋中胆固醇含量比较

<正>鸡蛋是人们日常生活中主要的食物来源之一,它富含蛋白质、卵黄素、卵磷脂等,同时又是人体所需的各种维生素和矿物元素的良好来源。然而鸡蛋的蛋黄中含有大量的胆固醇,过多的摄入可引起高血脂、血栓进而引发动脉粥样硬化等一系列心血管疾病[1]。随着人们保健意识的增强,希望能少摄入高胆固醇的食品。     

番茄抗叶霉病基因Cf19的抗性特点及遗传连锁分析

番茄抗叶霉病基因Cf19的抗性范围基本涵盖了中国目前分化出的所有叶霉菌生理小种,且抗性显著;台盼蓝染色结果显示该基因介导的抗性应答中出现明显的超敏反应,强度介于Cf4和Cf9基因之间;遗传连锁分析说明该基因的遗传特性符合单基因显性遗传规律;通过进一步SSR和AFLP分子标记连锁分析,共找到6个与Cf19基因连锁的分子标记,并将该基因初步定位于番茄1号染色体短臂区域。     

N2O production in the organic and mineral horizons of soil had different responses to increasing temperature

Journal of Soils and Sediments - The responses of N2O emission to increasing temperature in different soil horizons are not clearly understood yet. Here, we investigated the effects of increasing...     

河西灌区田间固定道保护性耕作下作物轮作模式效益评价

在甘肃河西走廊,通过田间试验结合入户调研的方式,运用综合评价的方法,对基于固定道保护性农业的7种轮作模式进行了分析。结果表明：小麦-加工型甜椒-玉米经济效益最好,净收益、产投比和单方水产值分别为1.52×10⁴元·hm^-2、2.00和5.39元·m^-3;小麦-啤酒大麦-小麦劳动生产率最高,达240.2元·人^-1·a^-1,社会效益在所有轮作模式中也相对最好;小麦-油葵-啤酒大麦区域水资源安全保障度、水分能量生产效率、热量利用率分别为1.83、2.07×10⁴ kJ·m^-3和66.12%,生态效益最好;综合效益相对较好的4种轮作模式分别为小麦-加工型甜椒-玉米、小麦-马铃薯-小麦、小 麦-玉米-玉米和小麦-针叶豌豆-小麦。兼顾考虑固定道保护性农业系统的稳定性维护,小麦-加工型甜椒-玉米、小麦-玉米-玉米和小麦-针叶豌豆-小麦三种轮作模式更适宜在固定道保护性农业技术体系中进行应用和推广。     

Effect of miR-181b on cell proliferation and expression of Bcl-2 and SP1 in HepG2 cells

AIM: To investigate the role of miR-181b in the expression of Bcl-2 and SP1 at mRNA and protein levels in the human hepatoma G2 cells (HepG2), and to explore the effect of miR-181b on the regulation of HepG2 cell proliferation. METHODS: The synthetic double-strand complementary DNA based on the sequence of miR-181b was inserted into the vector of miRNASelect^TM pEGP-miR. The microRNA high-expression plasmid was cloned, and the sequences were identified. The miR-181b plasmid was transfected into HepG2 cells with liposomes. The stable cell line was screened by puromycin. The mRNA and protein levels of Bcl-2 and SP1 were measured by RT-PCR and Western blotting, respectively. Methyl thiazolyl tetrazolium (MTT) method was used to analyze the proliferation of HepG2 cells. RESULTS: The Western blotting results showed that miR-181b inhibited the protein expression of Bcl-2 and SP1. The result of RT-PCR also indicated that the mRNA expression of Bcl-2 and SP1 was suppressed. Compared with the control, the growth rate of HepG2 with high expression of miR-181b was significantly decreased.CONCLUSION: miR-181b inhibits the proliferation of HepG2, which may be related to the down-regulation of Bcl-2 and SP1.     

内蒙古草甸草原生物量碳分配格局

以中国北方草甸草原为研究对象,借助GIS等工具手段,在草地群落类型实测数据基础上,对内蒙古草甸草原的地上/地下生物量分配开展定量研究,主要结论如下:1)内蒙古草甸草原的生物量碳密度为660.43 g C/m²,其中地榆群落(460.63 g C/m²)具有最高的地上生物量碳密度,地榆群落(787.10 g C/m²)和五花草塘群落(776.22 g C/m²)具有最高的地下生物量碳密度,其他群落间则不存在显著性差异(P>0.05);2)温带草甸草原以16.60×10⁶ hm²的面积,贡献了111.20 Tg的生物量碳,其中地上生物量碳为27.57 Tg,地下生物量碳为83.63 Tg,根冠比(R∶S)为3.03;3)地下生物量沿土壤深度的分布可分为两种类型:线叶菊、芨芨草、野大麦、地榆、贝加尔针茅、苔草以及五花草塘群落属于“指数型”,其地下生物量主要分布在0~10 cm土壤层,且符合指数函数,该类型占据草原群落的主要部分;拂子茅、小叶锦鸡儿以及芦苇群落属于“抛物线”形,其地下生物量主要分布在0~10 cm和20~40 cm土壤层,该类别群落主要为灌木或半灌木,分布曲线不符合指数函数而符合二次函数。