热带小奥德蘑深层发酵工艺研究

通过对热带小奥德蘑深层发酵培养基碳源、氮源和无机盐的筛选 ,利用正交试验得到了优化发酵培养基配方 ,在此基础上对发酵条件进行了探索。试验结果表明 ,热带小奥德蘑的适宜发酵培养基为 :葡萄糖 3%,蛋白胨 0 .4 %,KH2 PO40 .1%,MgSO4·7H2 O 0 .2 %,VB10 .0 1%。其适宜发酵温度为 2 8℃ ,起始pH5 .0～ 6.0 ,摇瓶装量 60mL 2 5 0mL ,接种量 10 %。     

在桩基础设计中两种规范的差异比较--《建筑地基基础设计规范》与《建筑桩基技术规范》

通过对二部规范的对比，阐明“地基基础”与“桩基”相比在桩基础设计中作了较大的调整，这样既节省了工程造价又提高了桩基础设计质量。     

加强我院职工队伍素质建设，积极参与市场竞争

国家林业局昆明勘察设计院职工队伍存在着：知识结构单一，缺乏复合型高级人才；离退休人员过多，职工负担过重；市场观念淡薄，竞争意识不强；功能单一，机制不活，职工队伍缺乏生机和活力等诸多问题。自1984年实行差额拨款后，已经采取了以下措施：建立“能上能下”的干部选拔任用机制；建立“能进能出”的人才管理机制；建立“能多能少”的弹性分配机制。并对今后工作提出了若干建议。     

海涂实行草地农业的综合生态学方法

依据海涂独特的自然属性确定开发利用方案是保证土地资源持续生产的关键。采用降低土壤盐分、实行保护性耕作、用植物残余物覆盖地面、种植牧草防止土壤侵蚀和促使生草层土的发育，保证了以潜在形式集中的富含必需营养元素的腐殖质在土壤中聚积，改善土壤结构，提高土壤水分和养分的利用效率，并以牧草饲养家畜的土地经营活动形成了成功的草地农业生产。     

结球莴苣“烧边”成因及其调控措施的研究进展

结球莴苣烧边病是生理性病害，严重影响其品质，制约生产。国内外多数研究者认为钙缺乏导致结球莴苣烧边病的发生，但是现在许多研究表明其他因素也能导致烧边病的出现，例如环境因素、生长速率、内源激素等，所以研究烧边病发生的机制及其与环境因素的关系是防御结球莴苣烧边病发生的关键。     

京郊露地蔬菜平衡施肥产量和养分数据库的建立

蔬菜施肥推荐需要了解作物的养分需求和收获部分带走的养分量。根据京郊地区露地蔬菜生产的调查结果,结合已有的相关文献和专家意见,建立了京郊露地主要栽培蔬菜的包括目标产量、经济产量、残茬产量、养分需求量、经济产量部分养分含量、作物残茬养分含量等内容的数据库。     

瓜类作物细菌性果实腐斑病(BFB)防治研究概述

瓜类细菌性果实腐斑病(BFB)是近年来威胁全球瓜类作物的严重病害。它主要通过种子带菌传播,寄主以西瓜、甜瓜为主,近年有向其他瓜类(南瓜)和蔬菜(番茄)等蔓延的趋势。其防治应分阶段,在种子田、育苗床和生产瓜田按要求进行,只有这样才能达到综合防治的效果。     

重庆市江北区城乡交错带土地利用规划探讨

特殊的经济地理位置使得城乡交错带成为土地利用规划和土地利用管理的重点和难点区域。本文以重庆市江北区为例，分析了城乡交错带土地利用的基本特性，并根据现状和未来15年国民经济和社会发展的预测。提出了土地利用总体规划的重点。     

Effects of matrix metalloproteinases on ventricular remodelingfollowing myocardial ischemia in the rat

AIM:To examine the relationship between the activity of matrix metallproteinases(MMPs) and ventricular remodeling following myocardial ischemia in the rat.METHODS:The model of myocardial ischemia(MI) in the rat was established by isoprenaline(ISP). The activity of MMPs was measured by zymography and collagen concentration was assessed by the method of chloramine T, so did I/III collagen ratio by immunohistochemical staining. The microstructure of myocardium was also observed by electron microscope.RESULTS:The activity of MMP-2 in myocardial ischemia group (group M) increased by 5.8 folds at 1 st week(P＜0.01), 2.3 folds at 2 nd week(P＜0.01) and 1.7 flods at 4 th week(P＜0.05) compared with control group (group C) and MMP-9's activity in group M increased by 4.9 folds (P＜0.01), 1.9 flods(P＜0.01) and 1.4 folds(P＜0.05), respectively. Collagen amount and I/III collagen ratio in group M increased compared with that in group C at 2 nd week and 4 th week. It showed that cardiac myocytes in group M were necrosed and collagen grew abundantly in interstitium under electron microscope.CONCLUSION:The activity of MMPs in the myocardial interstitium increased following myocardial ischemia, then collagen amount and I/III collagen ratio increased, which may be the major causes of ventricular remodeling.     

Influence of ischemia-reperfusion on apoptosis of sinoatrial node cells in rabbits in vivo

AIM:To study influence of ischemia-reperfusion(IR) on apoptosis and expression of apoptosis-related genes Fas-L, Bax and Bcl-2 of sinoatrial node(SAN) cells in rabbits in vivo. METHODS:Ninety healthy adult rabbits were divided randomly into control group, ischemia groups (I_{10 min}, I_{30 min}, I_{60 min} and I_{120 min}) and IR groups (I_{10 min}R_4h, I_{30 min}R_4h, I_{60 min}R_4hand I_{120 min}R_4h). IR injury model of SAN was established by occluding and loosening the start section of right coronary artery. The apoptosis of SAN cells was detected by TUNEL staining. The expression of Fas-L, Bax and Bcl-2 of SAN cells was detected by immunohistochemistry. RESULTS:①No obvious apoptosis of SAN cells was observed in control group, I_{10 min} and I_{30 min} groups. Apoptosis of different degrees in SAN cells were found in 68.3%(41/60) rabbits in I_{60 min}, I_{120 min} and 4 subgroups of IR. ②The highest expression of Fas-L and Bax was observed in I_{120 min} group and that of Bcl-2 was in I_{60 min} group. ③The highest expression of Fas-L and Bax was observed in I_{60 min}R_4h group. The peak level of Bcl-2 was observed in I_{30 min}R_4h group. ④The expression of Fas-L and Bax was significant higher in IR group than that in ischemic group at the same time point. CONCLUSION:Ischemia and IR induced apoptosis of SAN cells in rabbit in vivo. Fas-L、Bax、Bcl-2 may participate in the regulation of apoptosis and the injury during IR aggravates the apoptosis of SAN cells.