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AIM: Studying the mechanism of protective role of metallothionein(MT) in hypoxic preconditioning(HPC) of cultivated rat cardiomyocytes. METHODS:Using the model of hypoxia/reoxygenation of cultivated rat cardiomyocytes. Determining the contents of MT, malonyldialdehyde (MDA)-metabolism product of lipid peroxidation and the activities of Na+-K+ATPase, Ca2+-Mg2+ATPase of cardiomyocytes 24h after HPC, also determining the relevant changes after using MT antibody. RESULTS: After 24 h in HPC, the contents of MT and activities of Na+-K+ATPase, Ca2+-Mg2+ATPase were obviously higher than those in the control and hypoxia/reoxygenation(P<0.05), and the contents of MDA were decreased remarkedly (P<0.01). Then after using MT antibody, the activities of two enzyme were progressively decreased and the contents of MDA were significantly higher than those in the control and MT antibody-free groups(P<0.01). CONCLUSION: HPC may induce excessive synthesis of MT, and MT can protect myocardial reoxygenation injury by eliminating lipid peroxidation and rising the activities of Na+-K+ATPase and Ca2+-Mg2+ATPase. 相似文献
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Yonghee Shin Masahiro Seguchi Masumi Koriyama Alim Isnansetyo 《European Journal of Forest Research》2010,129(6):1237-1245
Leaf area index (LAI) is an important parameter to identify the water balance in forested watershed as a biological factor
influencing directly on the evapotranspiration in the forest area. The purpose of this study was to estimate the LAI in a
small forested watershed in summer and winter by applying the Terra/Advanced Spaceborne Thermal Emission and Reflection Radiometer
(ASTER) data to the LAI estimation method. In this study, the estimation was based on the absorption and scattering processes
of the solar radiation in the vegetation canopy and the spectral reflectance characteristics of soil vegetation. First, we
estimated LAI based on Price’s model by application of ASTER data on the forested watershed located in the Tenzan Mountains
of Saga, Japan. To validate the results of LAI estimation, secondly, we compared them to the measured LAI obtained by a plant
canopy analyzer (LAI-2000) on the observation area inside the target region. This study showed that the LAI estimation method
was a feasible and accurate method as indicated by the high relationship (r = 0.97) between LAI derived from ASTER data and LAI measured by LAI-2000. This paper is the first report on LAI estimation
using Terra/ASTER data based on Price’s model and field investigation. This LAI estimation method is a reliable and applicable
method. 相似文献
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