Effects of Oral Administration of Specific Antibodies to Rainbow Trout Oncorhynchus mykiss

A new product called oralized fish serum concentrate (OFSC) was evaluated for a possible effect against various bacterial pathogens in rainbow trout. The OFSC produced from immune trout sera was found to contain fully functional antibodies and complement component C3. The antibodies detected in the serum concentrate were specific to Vibrio anguillarum (O1 and O2) and Aeromonas salmonicida , which had been used for vaccination of the fish prior to serum collection. The functionality of the specific antibodies in OFSC was not reduced after 6 wk storage at -20 C, 5 C, and 20 C. The serum was mixed with commercial trout feed and used for feeding rainbow trout fry (first feed period). After oral delivery of OFSC to rainbow trout for 1 mo, samples of gut content and gut tissue contained functional antibodies. In gutted fish no functional antibodies were found. This suggests that antibodies from OFSC are unable to be transferred across the gut wall in a functional state. Oral administration of OFSC did not increase survival of rainbow trout in an immersion challenge with Vibrio anguillarum .     

Comparison of greenhouse and environmentally controlled growthroom root growth potential testing of 2+0 eastern white pine seedlings

Two-year-old estern white pine (Pinus strobus L.) seedlings were measured for Root Growth Potential (RGP) in a hydroponic system over two years in both a greenhouse and an environmentally controlled growthroom. Analyses of variance showed no significant differences (alpha = 0.05) between testing environments for either year. This study demonstrates the validity of using greenhouse RGP testing for 2+0 eastern white pine.     

Vepsuche zur Ermittlung der Inkubationszeit der Tödlichen Vergilbung der Kokospalme (Lethal Yellowing Disease of coconut palm) in der Wirtspflanze

Zusammenfassung Das Aussetzen von 24 Serien von Palmen in Kübeln in einem Krankheitsgebiet für eine beschränkte Zeit, 7 Sätze von 15–20 Palmen für einen Monat, 3 Sätze für zwei Monate und 14 Sätze für drei Monate, diente zur Ermittlung der Inkubationszeit für die Tödliche Vergilbung der Kokospalme. In den meisten Fällen, die zur Ansteckung im Krankheitsgebiet führten, lag die geschätzte Inkubationszeit zwischen 3 und 6 Monaten; nach dem aufgestellten Histogramm betrug die durchschnittliche Inkubationszeit 150–160 Tage. Die lange Expositionszeit bei der größeren Zahl der Sätze erlaubt keine genaue Festlegung der Zeitspanne. Nach Entfernen von Käfigen in einem zweiten Versuch, die als Schutz gegen Infektionen dienten, traten erstmals Symptome an Palmen nach 6 (1 Palme) und 7 (2 Palmen) Monaten auf. Unter Berücksichtigung, daß der Ausbruch einer Krankheit Schwankungen unterworfen ist und auß erdem wesentlich vom Infektionszeitpunkt abhängt, fügen sich diese Daten gut in die für die Inkubationszeit angenommene Zeitspanne ein.Bodenorganismen (Nematoden) scheiden als Überträger der Tödlichen Vergilbung aus, da die Palmen in Kübeln keinen Bodenkontakt hatten. Die Palmen in den Käfigen dagegen konnten, soweit sie in den Boden gepflanzt waren, mit ihren Wurzeln unter den Käfigrand vordringen und wären dadurch der Infektion zugänglich gewesen.Da nur in den Sätzen, die den Infektionsbedingungen eines Krankheitsgebietes zwischen den Monaten Februar und August ausgesetzt waren, einzelne Palmen in den Gruppen erkrankten, dürfte in dieser Zeitspanne der Ü berträger besonders aktiv oder in der übrigen Zeit des Jahres relativ selten sein, anders wäre die Häufung der Infektionen in diesen Monaten nicht zu erklären.

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Summary A transfer experiment using 17 sets of coconut palms, planted in drums and transferred bimonthly or three-monthly from a healthy into a diseased area (2 or 3 months exposure time) and 7 sets exposed for only a month, provided us with good information about the incubation period of Lethal Yellowing in a palm. The most probable incubation period ranges between 3 and 6 months; according to the dates on the histogram the mean incubation period appeared to be 150–160 days. The long exposure time used for most of the sets does not allow a more accurate assessment of this time interval.In a second experiment, groups of coconut palms which had been protected by cages for at least a year against infections were exposed to the natural infection conditions by removal of the cages. The first case of disease was recognized 6 months after cage removal, 2 additional cases a month later. Taking into consideration that the development of symptoms varies within a time interval depending upon the date of infection, growing conditions etc, this observation agress with the inferred incubation period gained in the transfer experiment.Soil organisms (nematodes o. a.) can be excluded as transmitters of the Lethal Yellowing pathogen. The palms used in the transfer experiment which later on showed symptoms were planted in drums; the roots having no connection with the soil in the field. The soil for filling the drums was collected in a healthy area. The roots of caged palms which were planted directly into the soil could penetrate under the cage border into the unprotected part of the plot which contained a lot of naturally diseased palms scattered across the field.Only sets of palms which were partly or fully exposed to the infection conditions of a diseased area during February to August developed a number of cases of Lethal Yellowing. The occurrence of infection during this period indicates this to be a time of particular vector activity.

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F.A.O.- Expert in Kingston, Jamaica; jetzt: Biologiche Bundesanstalt für Land- und Forwirschaft, Berlin-Dahlem.

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F.A.O.- Associate-Expert in Kingston (Coconut Industry Board.

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Biologische Bundesanstalt für Land- und Forstwirstchaft, 1 Berlin 33 Dahlem, Königin-Luise-Straße 19.     

Zur Repellentwirkung von Königskerzen auf Hausmäuse

Zusammenfassung Nach einer Mitteilung aus dem Ende des 18. Jahrhunderts sollen sich in Blüte stehende Pflanzen vonVerbascum thapsus als Mittel zur Vertreibung vonMus musculus undRattus norvegicus eignen. Eine Nachprüfung dieser Empfehlung mitV. thapsus, lychnitis undthapsiforme anMus verlief negativ.

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Summary At the end of the eighteenth centuryGoeze reports about the action ofVerbascum thapsus as a repellent againstMus musculus andRattus norvegicus. A revision of this information withV. thapsus, lychnitis andthapsiforme andMus musculus as test animals does not confirm his findings.

     

Is the metabolic syndrome a useful clinical concept in dogs? A review of the evidence

The metabolic syndrome is a set of risk factors for the development of type 2 diabetes, atherosclerosis, coronary heart disease and stroke in human beings. The term has recently been applied to dogs that exhibit components of the human metabolic syndrome, specifically visceral obesity, hypercholesterolaemia, hypertriglyceridaemia, hypertension and fasting hyperglycaemia. Obese dogs, like obese humans, are known to develop resistance to the glucose-lowering effects of insulin, and develop increased circulating concentrations of triglycerides, cholesterol and blood pressure. Unlike humans, however, obese dogs do not develop fasting hyperglycaemia or atherogenic hyperlipidaemia. Importantly, there is no evidence that dogs develop type 2 diabetes. Atherosclerosis, coronary heart disease and stroke are rare and not known to be associated with obesity in dogs. On the basis of current knowledge, the use of the term ‘metabolic syndrome’ in dogs does not appear to have merit.     

吡喹酮对鲤鱼感染台湾棘带吸虫后期囊幼虫的防治效果

[目的]探讨3种不同药物处理方式对台湾棘带吸虫引起的鲤鱼幼鱼急性鱼鳃传染病(开放性鳃病)的防治效果.[方法]在试验初期,将1620条鲤鱼幼鱼(70日龄)鱼鳃自然感染台湾棘带吸虫,随后分成4组,分别采用CuSO4、福尔马林和吡喹酮不同方式处理6周,与对照(自然喂食、不使用任何化学药剂)比较防治鲤鱼幼鱼急性鳃传染病的效果.[结果]感染台湾棘带吸虫的幼鱼生长缓慢,其身长和体重增长均较慢.施药后,幼鱼台湾棘带吸虫后期囊幼虫感染强度减少.在每千克饲料中分别添加50和75 mg吡喹酮喂食幼鱼5d,可以杀死鱼鳃中所有的台湾棘带吸虫后期囊幼虫,且鱼鳃恢复正常;面使用25 mg吡喹酮,仅有35%的后期囊幼虫死亡.所有不同浓度CuSO4处理(0.3、0.4和0.5mg/kg冲洗浸泡24 h及以3.0、4.0和5.0 mg/kg冲洗浸泡10m)和福尔马林处理(20、25和30 mg/kg冲洗浸泡及200、250和300 mg/kg短时间冲洗浸泡)均不能杀死台湾棘带吸虫后期囊幼虫.[结论]经普通化学药剂CuSO4或福尔马林冲洗浸泡,不能防治由台湾棘带吸虫后期囊幼虫引起的鲤鱼开放性鳃病,但50~75 mg吡喹酮/kg饲料可以在5d内治疗该病.     

Variability in Arabinoxylan,Xylanase Activity,and Xylanase Inhibitor Levels in Hard Spring Wheat

Arabinoxylans (AX), xylanase, and xylanase inhibitors have an important role in many cereal food processing applications. The effects of genotype, growing location, and their interaction (G × L) on AX, apparent xylanase activity, and apparent xylanase inhibition activity of Triticum aestivum xylanase inhibitor (TAXI) and xylanase inhibiting protein (XIP) were investigated for six hard red and six hard white spring wheat genotypes grown at three locations. Difference in total AX level among genotypes was not determined to a significant level by genotype. Instead, variability in total AX content was largely dependent on G × L. However, total AX content was significantly different between the two wheat classes. For bran xylanase activity, 25% of the variability could be attributed to G × L interaction. Moreover, there was significant difference between the bran xylanase activities in the two wheat classes. Bran TAXI activity and XIP activity were significantly different among genotypes. Genotype contributed 72% to the variability in TAXI activity and 39% in XIP. However, no significant difference was observed among the two wheat classes for TAXI or XIP activity. These results indicate that TAXI might be a stable parameter in segregating wheat genotypes with varying xylanase activity.     

Die Ebersberger Waldordnung aus dem 13. Jahrhundert

Ohne Zusammenfassung     

Analgesic efficacy of preoperative administration of meloxicam or butorphanol in onychectomized cats

OBJECTIVE: To determine analgesic efficacy and adverse effects of preemptive administration of meloxicam or butorphanol in cats undergoing onychectomy or onychectomy and neutering. DESIGN: Randomized controlled study. ANIMALS: 64 female and 74 male cats that were 4 to 192 months old and weighed 1.09 to 705 kg (2.4 to 15.5 lb). PROCEDURE: Cats received meloxicam (0.3 mg/kg [0.14 mg/lb], s.c.) or butorphanol (0.4 mg/kg [0.18 mg/lb], s.c.) 15 minutes after premedication and prior to anesthesia. A single blinded observer measured physiologic variables, assigned analgesia and lameness scores, and withdrew blood samples for each cat at baseline and throughout the 24 hours after surgery. Rescue analgesia (butorphanol, 0.4 mg/kg, i.v. or s.c.) or administration of acepromazine (0.025 to 0.05 mg/kg [0.011 to 0.023 mg/lb], i.v.) was allowed. RESULTS: Meloxicam-treated cats were less lame and had lower pain scores. Cortisol concentration was higher at extubation and lower at 1, 5, and 12 hours in the meloxicam-treated cats. Fewer meloxicam-treated cats required rescue analgesia at 3, 5, 12, and 24 hours after extubation. General impression scores were excellent or good in 75% of meloxicam-treated cats and 44% of butorphanol-treated cats. There was no treatment effect on buccal bleeding time; PCV and BUN concentration decreased in both groups, and glucose concentration decreased in meloxicam-treated cats. CONCLUSIONS AND CLINICAL RELEVANCE: Preoperative administration of meloxicam improved analgesia for 24 hours without clinically relevant adverse effects in cats that underwent onychectomy or onychectomy and neutering and provided safe, extended analgesia, compared with butorphanol.