Culture of frozen/thawed Bovine Demi-Embryos with Bovine Oviduct Epithelial Cells*

A method for in vitro culture of splitted frozen/thawed bovine embryos is described. These demi-embryos are of a low viability, but they survive the treatments, which lead to the final cultivation. The culture system gave a development of 41%of demi-embryos and 75 % of the whole embryos, and it is concluded, that this system is usable to culture demi-embryos.     

Effects of feeding Kentucky 31 tall fescue seed infected with acremonium coenophialum to laboratory rats

An endophytic fungus found in Kentucky 31 (KY-31) tall fescue has been associated with the "summer syndrome" (fescue toxicity) in grazing cattle. Four experiments were conducted to investigate the use of weanling rats as an animal model to study the fescue toxicity syndrome. When graded levels of fungus-infected (INF) or fungus-free (FF) KY-31 seed were fed, feed intake and growth rates were inversely related to the amount of INF seed in the diet. When diets containing 50% seed were fed, rats eating INF seed had reduced (P less than .05) daily water consumption (9 vs 20 ml) and lowered (P less than .05) body temperatures (36.8 vs 37.7 C) when compared with the FF treatments. Autoclaving the seed increased feed intake and growth rate of rats receiving both INF and FF seed diets, but daily gains continued to be less (P less than .05) in the rats fed autoclaved INF diets when compared with rats fed autoclaved FF diets (2.3 vs 6.8 g). Rats fed diets containing either 40% shredded wheat on which the fescue endophyte had been grown or 40% shredded wheat that was not innoculated did not differ in performance. Crude fiber, nitrogen-free extract, gross energy and dry matter digestibilities were enhanced (P less than .05) by the presence of INF seed in the diets when compared with the FF seed treatments. Crude protein and ether extract digestibilities and apparent biological value of fescue protein were not affected by presence of the fungus. The effect of INF seed on organ weights was inconsistent.(ABSTRACT TRUNCATED AT 250 WORDS)     

Gastric dilatation-volvulus associated with diaphragmatic hernia in three cats: clinical presentation, surgical treatment and presumptive aetiology

Three cats were examined because of acute dyspnoea and sudden abdominal enlargement. In all cats, radiographs revealed gastric dilatation-volvulus (GDV) and diaphragmatic hernia. Cardiovascular shock and dyspnoea were treated by intravenous fluid-therapy, oxygen administration and relief of diaphragmatic pressure by means of stomach decompression and in one case placing the patient in an inclined position. Gastric decompression was performed by needle gastrocentesis, placement of a rhino-gastric tube, or a combination of these. Diaphragmatic herniorrhaphy was performed in either case; one cat also underwent gastropexy. The immediate postoperative period resolved uneventfully and the cats were doing well at follow-up. Feline GDV is a rare event in which diaphragmatic hernia may be a predisposing factor.     

Zum Stand der Kenntnisse überProstephanus truncatus (Horn) (Col., Bostrichidae), einen gef?hrlichen Maissch?dling in Afrika

Es wird ein gedrängter Überblick über die Lebens- und Schadensweise sowie Möglichkeiten der Bekämpfung des BostrichidenProstephanus truncatus gegeben, der nach seiner Einschleppung aus Mittelamerika in Afrika zu einem gefürchteten Maisschädling werde. Besonders zahlreich vermehrt er sich in belieschten Maiskolben, eine in ländlichen Regionen der Tropen häufig praktizierte Lagerform. Neuere Untersuchungen deuten darauf hin, daß der Käfer die Kolben an der Pflanze schon vor der Einlagerung befällt.Die Verluste in Maislägern mitP. truncatus-Befall liegen durchschnittlich 4fach über dem sonst üblichen Niveau. Schätzungen ergaben, daß eine landesweite Ausbreitung des Schädlings in Kenia, das durch seine direkte Nachbarschaft mit dem außerordentlich stark betroffenen Tansania potentiell gefährdet ist, zu einem Ernteverlust von mindestens DM 33 Mio. pro Jahr führen würde. Dieser Ausfall müßte durch entsprechend hohe Importe ausgeglichen werden.     

Organic carbon-14 in the Amazon river system

Coarse and fine suspended particulate organic materials and dissolved humic and fulvic acids transported by the Amazon River all contain bomb-produced carbon-14, indicating relatively rapid turnover of the parent carbon pools. However, the carbon-14 contents of these coexisting carbon forms are measurably different and may reflect varying degrees of retention by soils in the drainage basin.