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31.
Agnieszka Brzozowska Michał RychłowskiAndrea D. Lipińska Krystyna Bieńkowska-Szewczyk 《Veterinary microbiology》2010
The Us3 gene is conserved among alphaherpesviruses and codes for a protein kinase, a multifunctional protein involved in many phases of virus infection, like nuclear egress, modulation of apoptosis and modification of the cellular cytoskeleton. Bovine herpesvirus (BHV-1), a member of the Alphaherpesvirinae, contains an open reading frame homologous to Us3 of other herpesviruses, which has been identified as a serine/threonine kinase (Takashima, Y., Tamura, H., Xuan, X., Otsuka, H., 1999. Identification of the Us3 gene product of BHV-1 as a protein kinase and characterization of BHV-1 mutants of the Us3 gene. Virus Res. 59, 23–34). To study the activity of BHV-1 Us3, we have cloned its sequence under control of the human cytomegalovirus (HCMV) promoter/enhancer and introduced it into a recombinant baculovirus (Bac Us3). Confocal microscopy analysis showed profound cytoskeletal modifications in various BHV-1-permissive and non-permissive cells transduced with BacUs3. We observed that Us3 expression changed cellular shape and induced formation of long microtubule-containing cell projections, a phenomenon which had also been observed in cells expressing pseudorabies virus Us3. The intracellular localization of Us3 was mostly nuclear but when the protein accumulated it could be detected in the cytoplasm, cell membranes and projections. Mutated forms of BHV-1 Us3 with point mutations near or within the kinase catalytic domain did not affect cell morphology indicating that kinase activity of BHV-1 Us3 is required for its cytoskeleton remodelling function. 相似文献
32.
Kowalska A Zakęś Z Siwicki AK Jankowska B Jarmołowicz S Demska-Zakęś K 《Fish physiology and biochemistry》2012,38(2):375-388
The aim of the study was to determine the impact of applying different proportions of linseed (LO) and sunflower (SFO) oils
in pikeperch diets on growth, histological changes in the liver, immunological and blood chemical parameters. The fish were
fed isoenergetic and isoprotein feeds containing SFO (group 100SFO) or LO (group 100LO) in quantities of 67 g kg/feed, and
a mixture of oils: 47 g SFO and 20 g LO kg/feed (group 70SFO/30LO) and 20 g SFO and 47 g LO kg/feed (group 30SFO/70LO). Dietary
ratios of polyunsaturated fatty acids from the n-3 and n-6 series (n3/n6 index) were 0.36–2.15. Pikeperch were reared for
56 days in three replicates for each dietary treatment. Various dietary oils and ratios of n3/n6 did not impact fish growth,
feed conversion ratio, viscerosomatic and hepatosomatic index, and size of the hepatocytes. Feeding the fish high quantities
of LO and SO oils (groups 100LO and 100SFO) reduced the immunological response of the phagocytes and lymphocytes in the fish.
Moreover, this resulted in significant differences among groups in the quantity of linolenic and linoleic acid in whole fish
bodies, viscera, fillets, and livers. Various quantities of vegetable oils in the fish diets did not impact the quantity of
arachidonic, eicosapentaenoic and docosahexaenoic acid in the fillets and livers. The immunological index and low quantities
of linoleic acid in the fillets obtained in group 30SFO/70LO indicate that the n3/n6 dietary ratio of 1.35 was the most advantageous
for feeding juvenile pikeperch feeds with vegetable oils. 相似文献
33.
Krystyna Przybyl 《Forest Pathology》1984,14(3):177-183
Differences in the development and rate of spread of C. fimbriata hyphae between susceptible, moderately resistant and resistant clones were observed. Conidia of various shapes and ascospores appeared in necrotic parts of shoots of susceptible and moderately resistant clones while in the resistant one only cylindrical conidia were present. 相似文献
34.
35.
Maciej Rożyński Andrzej Kapusta Krystyna Demska-Zakęś Marek Hopko Agnieszka Sikora Zdzisław Zakęś 《Fish physiology and biochemistry》2017,43(4):999-1010
The aim of this work was to determine the impact of surgically implanted telemetry transmitters (TTs) on the growth, survival, hematological and biochemical indexes, and wound healing in juvenile pikeperch (Sander lucioperca) (body weight 60–90 g). Two incision suturing methods were used—silk sutures (experiment I—group ST) or tissue adhesive (experiment II—group GT). After tagging, the fish were held in a recirculating system for 35 days. No statistically significant differences were noted in the growth or condition indexes analyzed among the fish tagged with TT compared with those from the control groups (untagged). Substantial individual variability was noted, however, in the parameters examined in both the control and tagged groups. Among the hematological indexes, statistically significant differences were only noted in experiment I. Lower values of mean corpuscular volume and mean corpuscular hemoglobin were noted in group ST. Among the biochemical parameters, creatinine was statistically significantly threefold lower, magnesium and alkaline phosphatase (ALP) levels were lower, and ammonia levels were higher in group ST than in the control group. In experiment II, significant differences were only noted for ALP. Tissue adhesive was the superior and more effective method for closing the incision after TT implantation in juvenile pikeperch. This type of suturing facilitated faster healing, and it had less of an impact on juvenile pikeperch welfare. 相似文献
36.
Beata Laczynska Krystyna Demska‐Zakes Mohammad Abdul Momin Siddique Dorota Fopp‐Bayat 《Aquaculture Research》2020,51(9):3623-3630
Larval growth and gonadal differentiation of gynogenetic sterlet Acipenser ruthenus were studied. The gynogenetic fish were produced by activation of albino sterlet eggs with UV‐irradiated sperm (collected from healthy coloration specimens), following by a heat shock of 34°C. Embryos were reared at 18.0 ± 0.5°C in a recirculation system and initially fed with Artemia sp. for 4 weeks and then fed with formulated feed. During the experiment, the length and weight of fish were recorded for the gynogenetic and control groups. Additionally, the analysis of gonadal sex differentiation was conducted with a 2‐week interval at 289, 303, 317, 331, 345 and 373 DPH. The mean body weight of the control group was significantly higher (p < .05) than the gynogenetic group only at 345 and 373 DPH, while the mean total body length was significantly higher at 331 and 345 DPH (p < .05). The gonad investigation was started at 289 DPH, and ~80% of females were found in the gynogenetic group during the trial period while the number of females and males was similar at 303, 331 and 373 DPH in the control group. The first symptoms of morphological differentiation appeared at 289 DPH, and the final shaping macroscopic structure of the gonad took place earlier in females than in males. 相似文献
37.
The influence of gamma-irradiation (32 kGy) followed by the addition of polysaccharides (potato starch, soluble potato starch, and sodium alginate) and heating on the properties of the films based on calcium caseinate (CC)-whey proteins isolate (WPI) and the gels formed with CaCl(2) was evaluated. Radiation induced an improvement of the mechanical and barrier properties of all films. The polysaccharides' effect on the irradiated and non-irradiated CC-WPI gels could be predicted as the sum of their separate effects on CC and on WPI, apart from the alginate interaction with the irradiated CC-WPI. The better properties of the films achieved after admixing polysaccharides to the formerly irradiated protein solution correspond to the smaller strength of gels. Properties of the films and gels prepared using the irradiated proteins and alginate differed depending on whether alginate was admixed before or after irradiation. Results were related to the protein structure, interaction with polysaccharides, and the film's microstructure. 相似文献
38.
Krystyna Bojarczuk Barbara Kieliszewska-Rokicka 《Water, air, and soil pollution》2010,207(1-4):227-240
A pot experiment was conducted to study the effects of high concentrations of available Cu and Pb in soil originated from the vicinity of a copper foundry in Poland (Cu, 2,585–3,725 mg kg?1 d.wt.; Pb, 1,459–1,812 mg kg?1 d.wt.) on the growth and chemical constituents of Betula pendula seedlings. Control plants grew in unpolluted forest soil. Dry matter accumulation in the plants during the growing season and root/leaf mineral content were determined. Colonization of birch roots by ectomycorrhizal (ECM) fungi also was evaluated, as was soil dehydrogenase activity for influence of the metals on soil microorganisms. The heavy metals negatively affected seedling growth, ECM colonization, and soil dehydrogenase activity. A reverse relationship was found between ECM abundance and heavy metal concentrations in birch leaves, indicating the potential of mycorrhizas to protect the aboveground part of young silver birch seedlings from elevated environmental levels of Cu and Pb. 相似文献
39.
Krystyna Danilewicz 《Forest Pathology》1980,10(5):296-306
The participation of epiphytic bacteria in the groups of bacteria dominating on the shoots and root collars of two year aspen and white poplar differs both in quantity and quality and depends on the chemical composition of the diffusion exudates related to cambium activity. This indicates that epiphytic bacteria can utilize carbon sources in the exudates and transform lignin precursors. 相似文献
40.
Mirosław Kobierski Krystyna Kondratowicz-Maciejewska Magdalena Banach-Szott Piotr Wojewódzki José Matias Peñas Castejón 《Journal of Soils and Sediments》2018,18(8):2777-2789