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41.
OBJECTIVE: To sequence the exonic and splice site regions of 5 cardiac genes associated with the human form of familial dilated cardiomyopathy (DCM) in Doberman Pinschers with DCM and to identify a causative mutation. ANIMALS: 5 unrelated Doberman Pinschers with DCM and 2 unaffected Labrador Retrievers (control dogs). PROCEDURES: Exonic and splice site regions of the 5 genes encoding the cardiac proteins troponin C, lamin A/C, cysteine- and glycine-rich protein 3, cardiac troponin T, and the beta-myosin heavy chain were sequenced. Sequences were compared for nucleotide changes between affected dogs and the published canine sequences and 2 control dogs. Base pair changes were considered to be causative for DCM if they were present in an affected dog but not in the control dogs or published sequences and if they involved a conserved amino acid and changed that amino acid to a different polarity, acid-base status, or structure. RESULTS: A causative mutation for DCM in Doberman Pinschers was not identified, although single nucleotide polymorphisms were detected in some dogs in the cysteine- and glycine-rich protein 3, beta-myosin heavy chain, and troponin T genes. CONCLUSIONS AND CLINICAL RELEVANCE: Mutations in 5 of the cardiac genes associated with the development of DCM in humans did not appear to be causative for DCM in Doberman Pinschers. Continued evaluation of additional candidate genes or a focused approach with an association analysis is warranted to elucidate the molecular cause of this important cardiac disease in Doberman Pinschers. 相似文献
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43.
Comparative survival and growth performance of European lobster larvae,Homarus gammarus,reared on dry feed and conspecifics 下载免费PDF全文
Adam Powell James Hinchcliffe Kristina Sundell Nils‐Gunnar Carlsson Susanne P. Eriksson 《Aquaculture Research》2017,48(10):5300-5310
A bottleneck of crustacean larval culture concerns nutrition and associated cannibalism in communal rearing systems, which impact on larval survival, development and growth. For early‐stage European lobster, Homarus gammarus larvae, feeding ecology and body composition are largely unknown. We initiated four progressive feeding experiments (novel feed types, feeding regime and feed size and cannibalism effects) on growth and survival, to inform and update husbandry protocols. Performance of larvae offered a dry commercial feed was not significantly different compared with a conventional wet plankton feed of the same ration and size grade (both within 600–1,000 μm). Further experiments found that the same ration of dry feed offered six times daily improved development and growth, over the conventional regime of three times daily. Small‐grade dry feed (particles: 250–360 μm) improved larval performance compared with a larger feed (360–650 μm). Larvae were also fed different proportions of dry feed and/or conspecifics in both communal and individual rearing systems (the latter preventing cannibalism via segregation). Individually reared larvae, fed only dead conspecifics, displayed the greatest survival (80%) to postlarvae. This underlines the impact of cannibalism on survival and nutrition in H. gammarus larviculture. A final experiment analysed H. gammarus zoea 1 composition, identifying deficiencies in ash and carbohydrate in lobster feeds. This suggests a need for a species‐specific, formulated dry feed for H. gammarus larviculture. Our research represents the first investigation of H. gammarus larval composition and dietary requirements and highlights decreased growth potential associated with providing nutrition solely from generic commercial feed. 相似文献
44.
Lothar Schmidt Kristina Warnstorff Hannelore Drfel Peter Leinweber Henning Lange Wolfgang Merbach 《植物养料与土壤学杂志》2000,163(6):639-648
Six forms of fertilizers and three rotations have been examined for 120 years in the ”︁Eternal Rye trial” in Halle (central German arid region, Haplic Phaeozem on sandy loess). Rank analysis, trend analysis and a new component model have been found to be suitable statistical methods to evaluate long‐term results without true replications. In this trial it was shown that mineral fertilization maintains like farmyard manuring the yield potential although at lower content of soil organic matter (SOM). Without fertilization, yield decline was greater with potato and silage maize than winter rye (Secale cereale L.). Deficiencies in N fertilization lead immediately, and in PK supply after more than 20 years, to a significant yield decrease which could be quickly compensated by full fertilization. Rye and maize monocultures also resulted in yield decreases. The establishing of new steady states in the turnover of SOM took about 50 years after changes in fertilization. Contrary to rye monoculture, both silage maize monoculture and potato alternating with winter rye caused considerable decomposition of SOM. According to analytical pyrolysis (Py‐FIMS and Py‐GC/MS), fertilization affects the SOM composition more than rotation. Without fertilization, a higher percentage of thermically stable SOM remained in comparison to the FYM soils. The introduction of potato into the rotation enhanced the content of easily decomposable SOM. 相似文献
45.
Giovanni Romagnoni Kristina
ie Kvile Knut‐Frode Dagestad Anne Maria Eikeset Trond Kristiansen Nils Chr. Stenseth
ystein Langangen 《Fisheries Oceanography》2020,29(4):324-339
The survival of fish eggs and larvae, and therefore recruitment success, can be critically affected by transport in ocean currents. Combining a model of early‐life stage dispersal with statistical stock–recruitment models, we investigated the role of larval transport for recruitment variability across spatial scales for the population complex of North Sea cod (Gadus morhua). By using a coupled physical–biological model, we estimated the egg and larval transport over a 44‐year period. The oceanographic component of the model, capable of capturing the interannual variability of temperature and ocean current patterns, was coupled to the biological component, an individual‐based model (IBM) that simulated the cod eggs and larvae development and mortality. This study proposes a novel method to account for larval transport and success in stock–recruitment models: weighting the spawning stock biomass by retention rate and, in the case of multiple populations, their connectivity. Our method provides an estimate of the stock biomass contributing to recruitment and the effect of larval transport on recruitment variability. Our results indicate an effect, albeit small, in some populations at the local level. Including transport anomaly as an environmental covariate in traditional stock–recruitment models in turn captures recruitment variability at larger scales. Our study aims to quantify the role of larval transport for recruitment across spatial scales, and disentangle the roles of temperature and larval transport on effective connectivity between populations, thus informing about the potential impacts of climate change on the cod population structure in the North Sea. 相似文献
46.
Krishna K. Thakur Raphaël Vanderstichel Karia Kaukinen Omid Nekouei Emilie Laurin Kristina M. Miller 《Journal of fish diseases》2019,42(4):533-547
In response to concerns that novel infectious agents were introduced through the movement of eggs as Atlantic salmon aquaculture developed in British Columbia (BC), Canada, we estimated the prevalence of infectious agents in archived return‐migrating Sockeye salmon, from before and during aquaculture expansion in BC (1985–94). Of 45 infectious agents assessed through molecular assays in 652 samples, 23 (7 bacterial, 2 viral and 14 parasitic) were detected in liver tissue from six regions in BC. Prevalence ranged from 0.005 to 0.83 and varied significantly by region and year. Agent diversity ranged from 0 to 12 per fish (median 4), with the lowest diversity observed in fish from the Trans‐Boundary and Central Coast regions. Agents known to be endemic in Sockeye salmon in BC, including Flavobacterium psychrophilum, Infectious haematopoietic necrosis virus, Ceratonova shasta and Parvicapsula minibicornis, were commonly observed. Others, such as Kudoa thyrsites and Piscirikettsia salmonis, were also detected. Surprisingly, infectious agents described only recently in BC salmon, Ca. Branchiomonas cysticola, Parvicapsula pseudobranchicola and Paranucleospora theridion, were also detected, indicating their potential presence prior to the expansion of the aquaculture industry. In general, our data suggest that agent distributions may not have substantially changed because of the salmon aquaculture industry. 相似文献
47.
48.
Daniel C Dunn Caroline Jablonicky Guillermo O Crespo Douglas J McCauley David A Kroodsma Kristina Boerder Kristina M Gjerde Patrick N Halpin 《Fish and Fisheries》2018,19(4):729-739
Between 1950 and 1989, marine fisheries catch in the open‐ocean and deep‐sea beyond 200 nautical miles from shore increased by a factor of more than 10. While high seas catches have since plateaued, fishing effort continues to increase linearly. The combination of increasing effort and illegal, unreported and unregulated (IUU) fishing has led to overfishing of target stocks and declines in biodiversity. To improve management, there have been numerous calls to increase monitoring, control and surveillance (MCS). However, MCS has been unevenly implemented, undermining efforts to sustainably use high seas and straddling stocks and protect associated species and ecosystems. The United Nations General Assembly is currently negotiating a new international treaty for the conservation and sustainable use of biodiversity beyond national jurisdiction (BBNJ). The new treaty offers an excellent opportunity to address discrepancies in how MCS is applied across regional fisheries management organizations (RFMOs). This paper identifies ways that automatic identification system (AIS) data can inform MCS on the high seas and thereby enhance conservation and management of biodiversity beyond national jurisdictions. AIS data can be used to (i) identify gaps in governance to underpin the importance of a holistic scope for the new agreement; (ii) monitor area‐based management tools; and (iii) increase the capacity of countries and RFMOs to manage via the technology transfer. Any new BBNJ treaty should emphasize MCS and the role of electronic monitoring including the use of AIS data, as well as government–industry–civil society partnerships to ensure critically important technology transfer and capacity building. 相似文献
49.
Christina A.D. Semeniuk Sophie Bourgeon Kristina D. Rothley 《Biological conservation》2009,142(8):1818-1829
Wildlife tourism alters the environmental conditions in which the focal animal lives, and it is therefore necessary to assess the ability of the animal to adjust to and persist in these novel conditions if the industry is to be sustainable. Here, we report on the physiological responses of southern stingrays (Dasyatis americana) which are the focus of intense marine provisioning-tourism in the Cayman Islands. Using stingrays from non-tourist sites about Grand Cayman as a basis for comparison, we show in this natural experiment that tourist-exposed stingrays exhibit hematological changes indicative of physiological costs of wildlife tourism. The novel conditions with which the stingrays must interact include non-natural food, higher injury rates (from boats, conspecifics and predators), and higher parasite loads (from crowding conditions). As a result of this year-round environment, stingrays display sub-optimal health: lower hematocrit, total serum protein concentrations, and oxidative stress (i.e., lower total antioxidant capacity combined with higher total oxidative status). Moreover, they show evidence of attenuation of the defense system: for tourist stingrays only, animals possessing both injuries and high parasite loads also exhibit lowest leukocrit, serum proteins and antioxidant potential, as well as differing proportions of differential leukocytes indicative of suppression (lymphocytes and heterophils) and down-regulation (eosinophils), thus suggesting that the physiological changes of tourist stingrays are in partial response to these stressors. While survival- and reproduction- quantification was not possible in this long-lived marine species, the physiological measures -situated within ecological context, indicate that the long-term health and survival of tourist stingrays have a significant probability of being affected. Consequently, management of the tourism attraction is essential. The indicators chosen in this study reflect general health indices and defense capabilities used across taxa, and represent a tradeoff between ease of collection/analysis and interpretation so that managers can continue the research for monitoring purposes. 相似文献
50.
Magdalene J. Seiler Robert B. Aramant† Mathias W. Seeliger‡ Ragnheidur Bragadottir§ Melissa Mahoney¶ Kristina Narfstrom 《Veterinary ophthalmology》2009,12(3):158-169
Purpose To investigate whether sheets of fetal retinal allografts can integrate into the dystrophic Abyssinian cat retina with progressive rod cone degeneration.
Methods Fetal retinal sheets (cat gestational day 42), incubated with BDNF microspheres, were transplanted to the subretinal space of four cats at an early disease stage. Cats were studied by fundus examinations, bilateral full-field flash ERGs, and indocyanine green and fluorescein angiograms up to 4 months following surgery. E42 donor and transplanted eyes were analyzed by histology and immunohistochemistry for retinal markers.
Results Funduscopy and angiography showed good integration of the transplants in two of four cats, including extension of host blood vessels into the transplant and some scarring in the host. In these two, transplants were found in the subretinal space with laminated areas, with photoreceptor outer segments in normal contacts with the host retinal pigment epithelium. In some areas, transplants appeared to be well-integrated within the host neural retina. Neither of these two cats showed functional improvement in ERGs. In the other two cats, only remnants of donor tissue were left. Transplants stained for all investigated cellular markers. No PKC immunoreactivity was detected in the fetal donor retina at E42, but developed in the 4-month-old grafts.
Conclusions Fetal sheet transplants can integrate well within a degenerating cat retina and develop good lamination of photoreceptors. Functional improvement was not demonstrated by ERG in cats with well-laminated grafts. Transplants need to be further evaluated in cat host retinas with a more advanced retinal degeneration using longer follow-up times. 相似文献
Methods Fetal retinal sheets (cat gestational day 42), incubated with BDNF microspheres, were transplanted to the subretinal space of four cats at an early disease stage. Cats were studied by fundus examinations, bilateral full-field flash ERGs, and indocyanine green and fluorescein angiograms up to 4 months following surgery. E42 donor and transplanted eyes were analyzed by histology and immunohistochemistry for retinal markers.
Results Funduscopy and angiography showed good integration of the transplants in two of four cats, including extension of host blood vessels into the transplant and some scarring in the host. In these two, transplants were found in the subretinal space with laminated areas, with photoreceptor outer segments in normal contacts with the host retinal pigment epithelium. In some areas, transplants appeared to be well-integrated within the host neural retina. Neither of these two cats showed functional improvement in ERGs. In the other two cats, only remnants of donor tissue were left. Transplants stained for all investigated cellular markers. No PKC immunoreactivity was detected in the fetal donor retina at E42, but developed in the 4-month-old grafts.
Conclusions Fetal sheet transplants can integrate well within a degenerating cat retina and develop good lamination of photoreceptors. Functional improvement was not demonstrated by ERG in cats with well-laminated grafts. Transplants need to be further evaluated in cat host retinas with a more advanced retinal degeneration using longer follow-up times. 相似文献