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101.
Timm RM Ramey RR;Nomenclature Committee of the American Society of Mammalogists 《Science (New York, N.Y.)》2005,309(5744):2163-6; author reply 2163-6
102.
103.
Weller GR Kysela B Roy R Tonkin LM Scanlan E Della M Devine SK Day JP Wilkinson A d'Adda di Fagagna F Devine KM Bowater RP Jeggo PA Jackson SP Doherty AJ 《Science (New York, N.Y.)》2002,297(5587):1686-1689
In eukaryotic cells, double-strand breaks (DSBs) in DNA are generally repaired by the pathway of homologous recombination or by DNA nonhomologous end joining (NHEJ). Both pathways have been highly conserved throughout eukaryotic evolution, but no equivalent NHEJ system has been identified in prokaryotes. The NHEJ pathway requires a DNA end-binding component called Ku. We have identified bacterial Ku homologs and show that these proteins retain the biochemical characteristics of the eukaryotic Ku heterodimer. Furthermore, we show that bacterial Ku specifically recruits DNA ligase to DNA ends and stimulates DNA ligation. Loss of these proteins leads to hypersensitivity to ionizing radiation in Bacillus subtilis. These data provide evidence that many bacteria possess a DNA DSB repair apparatus that shares many features with the NHEJ system of eukarya and suggest that this DNA repair pathway arose before the prokaryotic and eukaryotic lineages diverged. 相似文献
104.
Partridge EA Le Roy C Di Guglielmo GM Pawling J Cheung P Granovsky M Nabi IR Wrana JL Dennis JW 《Science (New York, N.Y.)》2004,306(5693):120-124
The Golgi enzyme beta1,6 N-acetylglucosaminyltransferase V (Mgat5) is up-regulated in carcinomas and promotes the substitution of N-glycan with poly N-acetyllactosamine, the preferred ligand for galectin-3 (Gal-3). Here, we report that expression of Mgat5 sensitized mouse cells to multiple cytokines. Gal-3 cross-linked Mgat5-modified N-glycans on epidermal growth factor and transforming growth factor-beta receptors at the cell surface and delayed their removal by constitutive endocytosis. Mgat5 expression in mammary carcinoma was rate limiting for cytokine signaling and consequently for epithelial-mesenchymal transition, cell motility, and tumor metastasis. Mgat5 also promoted cytokine-mediated leukocyte signaling, phagocytosis, and extravasation in vivo. Thus, conditional regulation of N-glycan processing drives synchronous modification of cytokine receptors, which balances their surface retention against loss via endocytosis. 相似文献
105.
Singha A Gibertini M Karmakar B Yuan S Polini M Vignale G Katsnelson MI Pinczuk A Pfeiffer LN West KW Pellegrini V 《Science (New York, N.Y.)》2011,332(6034):1176-1179
Artificial crystal lattices can be used to tune repulsive Coulomb interactions between electrons. We trapped electrons, confined as a two-dimensional gas in a gallium arsenide quantum well, in a nanofabricated lattice with honeycomb geometry. We probed the excitation spectrum in a magnetic field, identifying collective modes that emerged from the Coulomb interaction in the artificial lattice, as predicted by the Mott-Hubbard model. These observations allow us to determine the Hubbard gap and suggest the existence of a Coulomb-driven ground state. 相似文献
106.
107.
T A Roy J R Meeks C R Mackerer 《Journal of the Association of Official Analytical Chemists》1983,66(6):1319-1321
Ion-pair reverse phase liquid chromatography (LC) and UV detection at 280 nm have been used to determine sodium acifluorfen (sodium-5-[2-chloro-4-(trifluoromethyl)-phenoxy]-2-nitrobenzoate), an experimental diphenyl ether herbicide, in dog feed. Sodium-5-(2,4-dichlorophenoxy)-2-nitrobenzoate is used as the internal standard. The feed is homogenized in 0.01N HCl, followed by ethyl acetate extraction, and centrifugation. The organic layer is removed and evaporated and the residue is reconstituted in methanol and filtered before LC analysis (mobile phase methanol-water (58 + 42), 0.005M in tetrabutylammonium phosphate and 0.045M in (NH4)2HPO4, at pH 7.4). The ion-pair technique offers a high degree of control over the retention characteristics of the herbicide and internal standard. The use of the internal standard permits precise and accurate quantitation and substantially reduces analysis time compared with the external standard method. 相似文献
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109.
Abha Jain A. K. Roy P. Kaushal D. R. Malaviya S. N. Zadoo 《Genetic Resources and Crop Evolution》2006,53(2):339-347
Isozyme banding pattern was studied in Guinea grass (Panicum maximum Jacq.), a widely cultivated grass having good fodder value. Similarity among 63 accessions collected from diverse sources
was worked out using five enzyme systems (SOD, GOT, ACP, Esterase and Peroxidase) following horizontal starch gel electrophoresis.
Biochemical markers such as isozymes are useful supplements in identifying the genetic variation present in any crop. A total
of 35 clear and unambiguous bands were used for analysis of which 8 bands were monomorphic. Polymorphism exhibited by 27 bands
from all five enzyme systems indicate presence of considerable diversity in this species. The dendrogram generated after UPGMA
and SAHN cluster analysis using Jaccard genetic distance showed that 63 accessions from diverse geographical locations could
be grouped in three main clusters, of which two could further be divided into sub-clusters. Although the clusters comprised
members from different locations, most of the accessions from similar geographical locations tended to cluster in same group. 相似文献
110.
This paper describes a pilot investigation dealing with changes in chemical composition of wood in pollution-stressed white pine trees, taken from two forested ecosystems of Southern Ontario, Canada. The implications of this research lie in a much needed search for a potential bloindicator of a pre-decline, incipient-stress induced (and/or contributed by) the air-pollutants. 相似文献