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51.
Sakai H Mori T Iida T Tokuma Y Maruo K Masegi T 《Journal of Feline Medicine and Surgery》2008,10(3):296-299
Feline inductive odontogenic tumour (FIOT) is a rare and interesting odontogenic neoplasm in which the odontogenic epithelium has inductive potential to form aggregated foci of dental pulp-like mesenchymal cells. Two male cats aged 11 and 10 months presented with nasal swelling and a left maxillary mass. Histopathologically, the masses consisted of non-encapsulated invasive neoplasms exhibiting proliferation of epithelial and mesenchymal components with local infiltration into the maxillary bone in both cases. The epithelial component formed islands, anastomosing strands, and solid sheets of polygonal epithelial cells. Occasionally, these cells formed circular aggregates, resembling the cap stage of odontogenesis. Type IV collagen and laminin were constantly positive around the foci of epithelial cells, and Ki-67 positive indices were extremely low; therefore, these findings consistent with the benign clinical presentation of FIOT. 相似文献
52.
Kasai K Sano F Miyashita N Watanabe S Nagai T 《The Journal of reproduction and development》2007,53(1):135-142
In the present study, the growth performance of a calf produced by mating a somatic cell cloned dam and sire was compared with that of its full siblings produced by mating the cattle used as nuclear donors for the cloned animals. The somatic cell cloned dam and sire were derived from cultured cumulus cells and ear cells, respectively. The cloned dam was artificially inseminated with semen from the cloned sire. A female calf was produced that was reared under general group feeding conditions. The calf was subjected to a clinical examination and to hematology, serum biochemistry, and telomere length analyses; all of these tests indicated that the calf was normal. The growth characteristics (body weight and shoulder height) of the calf fell within the range of the full siblings of the same sex produced by mating the animals used as the nuclear donors of clones. These findings suggest that the same breeding performance is expected from mating a cloned dam and sire as from mating the animals used as nuclear donors for the clones. 相似文献
53.
Sakai K Sakabe G Tani O Watanabe Y Jahangir A Nakamura M Takehara K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(12):1307-1311
A field isolate of Newcastle disease virus (NDV) isolated from northern pintail (Anas acuta) in Tohoku district, northeast Japan, was characterized. Phylogenetic analysis of the fusion protein indicated that the isolate belonged to genotype I and was closely related to isolates from the Far East corresponded to the migration route for this bird species. The isolate had the typical avirulent cleavage site of the fusion protein (112)GKQGR*L(117). In addition, pathogenicity tests indicated the isolate to have avirulent characteristics. However, the isolate has been shown to cause fusion cytopathic effects and form plaques on chicken embryo fibroblasts (CEF) in the absence of trypsin. The present results suggest that the CEF-adapted NDV, which is avirulent, is circulating among waterfowl populations. 相似文献
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Heshiko extract was separated into peptide and amino acid fractions by ion-exchange column chromatography. Heshiko extract and these fractions were administered to rats in a diet enriched with lipid and cholesterol for 30 days. In the heshiko extract group, increase of triglyceride, total cholesterol, and low-density lipoprotein (LDL)-cholesterol levels in plasma
and accumulation of total lipids in the liver were suppressed, while amounts of both lipid and cholesterol excreted to feces
were increased. Administration of heshiko extract tended to increase fecal bile acids and promoted the activity of cholesterol 7α-hydroxylase, the rate-limiting enzyme
in the synthesis of bile acid from cholesterol in the liver. However, activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA)
reductase, the rate-limiting enzyme in the cholesterol synthesis system in the liver, decreased due to regulation by the feedback
of lipid transportation from diet to the liver. The same effect was observed in the peptide and free amino acid fraction groups.
These data suggest that both excretion of cholesterol and bile acid to feces and promotion of cholesterol 7α-hydroxylase activity
are related to the hypocholesterolemic effects of heshiko extract and that both peptides and amino acids contribute to that effect. 相似文献
56.
To investigate the extent of genetic differentiation among wild populations of the Pacific cod Gadus macrocephalus, we have examined genetic polymorphism at five locations within Korean waters [Boryeong in the West Sea (WC-BR); Jinhae Bay
in the South Sea (SC-JH); Jumunjin (EC-JM), Jukbyeon (EC-JB), and Bangeojin (EC-BJ) off the eastern coast of Korea] using
mitochondrial DNA (mtDNA) control region and microsatellite DNA (msDNA) markers. Nucleotide sequence analysis of 584 bp in
the variable portion of the 5′ end of the mtDNA control region revealed 27 variable nucleotide sites among 184 individuals,
which defined eight, three, and 11 haplotypes in the western, southern, and eastern coast populations, respectively. The mtDNA
analysis revealed a low variability but significant local differentiation among populations from these three areas within
Korean waters. msDNA analysis also revealed moderate polymorphism in the wild populations, with a mean of 13.8–22.6 alleles
per locus for the five msDNA markers and observed (and expected) heterozygosities of 0.755 (0.825) for the WC-BR, 0.793 (0.810)
for the SC-JH, 0.920 (0.905) for the EC-BJ, 0.783 (0.865) for the EC-JB, and 0.804 (0.812) for the EC-JM populations. Analysis
of msDNA loci indicated that Pacific cod sampled at the WC-BR, SC-JH, and EC-JB sites belong to genetically distinct populations.
However, no significant difference was found between the Pacific cod population from SC-JH and that from EC-BJ. Consequently,
three genetically distinct populations, namely, WC-BR, SC-JH and EC-BJ, and EC-JB, were identified using msDNA analysis. These
results indicate that genetically distinct populations of Pacific cod are present in Korean coastal waters where spawning
aggregations occur. 相似文献
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Satoshi Kambayashi Kouji Minami Yuka Ogawa Takehiro Hamaji Chung Chew Hwang Masaya Igase Hiroko Hiraoka Takako Shimokawa Miyama Shunsuke Noguchi Kenji Baba Takuya Mizuno Masaru Okuda 《Canadian journal of veterinary research》2015,79(3):201-209
The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) causes resistance to nitrosoureas in various human cancers. In this study, we analyzed the correlation between canine lymphomas and MGMT in vitro. Two of five canine lymphoma cell lines required higher concentrations of lomustine to inhibit cell growth by 50%, but their sensitivity to the drug increased when they were cultured with an MGMT inhibitor. Fluorometric oligonucleotide assay and real-time polymerase chain reaction of these cell lines revealed MGMT activity and high MGMT mRNA expression, respectively. We analyzed the methylation status of the CpG islands of the canine MGMT gene by the bisulfite-sequencing method. Unlike human cells, the canine lymphoma cell lines did not show significant correlation between methylation status and MGMT suppression levels. Our results suggest that in canine lymphoma MGMT activity may influence sensitivity to nitrosoureas; thus, inhibition of MGMT activity would benefit nitrosourea-resistant patients. Additional studies are necessary to elucidate the mechanism of regulation of MGMT expression. 相似文献
59.
Misawa E Tanaka M Nomaguchi K Nabeshima K Yamada M Toida T Iwatsuki K 《Journal of agricultural and food chemistry》2012,60(11):2799-2806
We investigated the effects of the oral administration of lophenol (Lo) and cycloartanol (Cy), two kinds of antidiabetic phytosterol isolated from Aloe vera , on glucose and lipid metabolism in Zucker diabetic fatty (ZDF) rats. We demonstrated that the administrations of Lo and Cy suppressed random and fasting glucose levels and reduced visceral fat weights significantly. It was also observed that treatments with Lo and Cy decreased serum and hepatic lipid concentrations (triglyceride, nonesterified fatty acid, and total cholesterol). Additionally, Lo and Cy treatments resulted in a tendency for reduction in serum monocyte chemotactic protein-1 (MCP-1) level and an elevation in serum adiponectin level. Furthermore, the expression levels of hepatic genes encoding gluconeogenic enzymes (G6 Pase, PEPCK), lipogenic enzymes (ACC, FAS), and SREBP-1 were decreased significantly by the administrations of aloe sterols. In contrast, Lo and Cy administration increased mRNA levels of glycolysis enzyme (GK) in the liver. It was also observed that the hepatic β-oxidation enzymes (ACO, CPT1) and PPARα expressions tended to increase in the livers of the Lo- and Cy-treated rats compared with those in ZDF-control rats. We therefore conclude that orally ingested aloe sterols altered the expressions of genes related to glucose and lipid metabolism, and ameliorated obesity-associated metabolic disorders in ZDF rats. These findings suggest that aloe sterols could be beneficial in preventing and improving metabolic disorders with obesity and diabetes in rats. 相似文献
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Tomoya MURAKAMI Satoshi AIDA Kouji YOSHIOKA Tetsuya UMINO Heisuke NAKAGAWA 《Fisheries Science》2004,70(5):839-844
ABSTRACT: This study is the first to report on the high occurrence of agglutinated pelvic fin membrane deformities in hatchery-reared black rockfish Sebastes inermis . For 5 years, this symptom was marked in hatchery-reared fish, with 58.7% of fish deformed on average (varying between 46.7 and 72.0%). The deformity was a peculiarity in hatchery-reared fish, but is negated in wild fish, and was not related to whether the fish broodstock originated from the wild or from a hatchery. Mark–release experiments showed that deformed fish were almost the same as normal hatchery-reared fish in growth and survival rates, and, theoretically, the recapture numbers of hatchery-stocked fish, estimated by deformity, almost coincided with actual recapture numbers, confirmed by otolith tagging. The results of the present study indicate that deformity in hatchery-reared black rockfish is useful as a stock separation tool. 相似文献