全文获取类型
收费全文 | 2130篇 |
免费 | 99篇 |
国内免费 | 262篇 |
专业分类
林业 | 209篇 |
农学 | 343篇 |
基础科学 | 180篇 |
369篇 | |
综合类 | 581篇 |
农作物 | 150篇 |
水产渔业 | 116篇 |
畜牧兽医 | 306篇 |
园艺 | 56篇 |
植物保护 | 181篇 |
出版年
2024年 | 3篇 |
2023年 | 16篇 |
2022年 | 54篇 |
2021年 | 94篇 |
2020年 | 80篇 |
2019年 | 69篇 |
2018年 | 68篇 |
2017年 | 77篇 |
2016年 | 78篇 |
2015年 | 110篇 |
2014年 | 100篇 |
2013年 | 104篇 |
2012年 | 111篇 |
2011年 | 139篇 |
2010年 | 133篇 |
2009年 | 124篇 |
2008年 | 124篇 |
2007年 | 128篇 |
2006年 | 132篇 |
2005年 | 106篇 |
2004年 | 54篇 |
2003年 | 51篇 |
2002年 | 39篇 |
2001年 | 47篇 |
2000年 | 54篇 |
1999年 | 59篇 |
1998年 | 54篇 |
1997年 | 39篇 |
1996年 | 39篇 |
1995年 | 43篇 |
1994年 | 26篇 |
1993年 | 28篇 |
1992年 | 23篇 |
1991年 | 14篇 |
1990年 | 20篇 |
1989年 | 18篇 |
1988年 | 6篇 |
1987年 | 12篇 |
1986年 | 5篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1976年 | 1篇 |
1974年 | 2篇 |
1964年 | 1篇 |
排序方式: 共有2491条查询结果,搜索用时 15 毫秒
41.
The material flow and bulk internal flow analyses were used to establish a material accumulation and cycling model for a low-quality forest stand improvement system and a series of processes were considered. The model was applied in a one-hectare low-quality forest plot in the Lesser Khingan Range of China. Results showed that during 1997–2007, the stands absorbed 270.19 kg of N, 74.28 kg of P, and 124.39 kg of K from soils, 51.82 kg of N and 2.38 kg of P were directly absorbed by foliage, and 16.25 kg of K was released to soils by eluviation. Until 2007, the accumulated nutrients in the stands included 236.91 kg of N, 65.28 kg of P, and 108.55 kg of K. When horizontal strip clearcutting was applied in 2007, 50% accumulated nutrients in the stands were shifted due to harvesting operations, and 212.74 kg of N, 26.97 kg of P, and 98.88 kg of K were accumulated in soils, declining by 9.47% for N, 3.68% for P, and 17.60% for K, respectively, compared with year 1997. 94.61 t per hectare of biomass was generated, of which the biomass in stands accounted for 87.36%. The felled tree biomass was 36.89 t per hectare, of which 84.90% and 10.03% of biomass were utilized in terms of logs and other means, and the rest was left on site. 相似文献
42.
Xiaodong Dai Yaguang Zhan Jiechi Zhang Piqi Zhang Zenghua Han Qingfang Ma Xianghui Kong Jianing Liu Yinpeng Ma 《林业研究》2015,(1):71-77
We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid(SA) and methyl jasmonic acid(Me JA)supplementation. The optimal SA concentration was100 lmoláL-1, where the highest ergosterol content of 2.33 mgág-1was obtained following 6-day cultivation with100 lmoláL-1SA supplementation, and which was significantly higher than the unsupplemented control(p \ 0.01). Following 4-day supplementation with50 lmoláL-1Me JA, the highest ergosterol content obtained was 1.988 mgág-1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and Me JA supplementation improves ergosterol content in H.erinaceus mycelium. 相似文献
43.
45.
46.
为验证自行研制的A型流感病毒分型基因芯片方法在实际中的应用效果,了解A型流感病毒各亚型在我国不同地区和不同宿主中的分布情况,对我国20个地区、不同宿主源(鸡、鸭、鹌鹑、鸽子、猪、人等)拭子和组织样品共16 852份进行了检测。利用自行研制的A型流感病毒分型基因芯片方法,对样品进行RNA提取、多重不对称RT-PCR扩增、芯片杂交、洗涤和扫描分析;同时将基因芯片检测阳性的样品,用普通RT-PCR扩增后进行序列测定。结果显示:利用该方法检出阳性样品2 582份,总检出率为15.32%(2 582/16 852),共检测到H1N1、H2N9、H3N2、H4N6、H5N1、H7N1、H7N9、H9N2、H10N5、H16N3等10个亚型,且均与阳性样品测序结果一致。结果表明,该方法可准确检测不同地区、不同宿主中的A型流感病毒不同亚型。同时,该方法能在同一张芯片上准确鉴定A型流感病毒的多种亚型,解决了其他常规方法无法检测已发现和可能发生变异的所有亚型的棘手问题,从而为A型流感诊断和分子流行病学监测提供了一种新技术。 相似文献
47.
Caihua Kong Kena Liu Qin Wang Rong Fu Huaxin Si Shiyan Sui 《Reproduction in domestic animals》2021,56(11):1413-1424
Oxidative stress can induce apoptosis of granulosa cells and lead to follicular atresia, thereby reducing the number of pigs giving birth. The aim of this study was to investigate the protective effect of Periplaneta americana peptide (PAP) on the apoptosis of the granulosa cells of pig ovaries (PGCs) induced by hydrogen peroxide (H2O2) via FoxO1. PGCs were treated with H2O2 to establish a cell apoptosis model. Cell viability was measured using the cell counting kit-8 (CCK-8) assay, and cell apoptosis was detected using flow cytometry. The malondialdehyde (MDA) level and nitric oxide (NO) content were detected to reflect the oxidative stress. Western blotting, qRT-PCR and overexpression were undertaken to determine the expression of FoxO1 and caspase-3, and immunofluorescence was used to detect FoxO1 in the nucleus and cytoplasm. PGCs were treated with 100 μM H2O2 for 6 hr, which resulted in oxidative damage and apoptosis and an apoptosis rate for PGCs of 32.95%. Next, PGCs were treated with 400 μg/ml PAP for 24 hr to repair the apoptosis induced by H2O2. PAP improved cell viability in H2O2-stimulated PGCs, the increased MDA level and NO content caused by H2O2 stimulation were reversed and the apoptotic rate of PGCs was reduced. The qRT-PCR and Western blotting results indicated that PAP decreased the H2O2-induced apoptosis and the expression of FoxO1 and caspase-3 in PGCs. The effect of PAP was the same following FoxO1 overexpression. FoxO1 was expressed in the nucleus when stimulated by H2O2 or overexpression; however, it migrated to the cytoplasm following PAP treatment. PAP decreased the apoptosis of PGCs induced by H2O2 by regulating FoxO1 expression and nuclear translocation. 相似文献
48.
为考察不同来源吸附载体大豆糖蜜对奶牛瘤胃内环境的影响,采用3×3拉丁方试验设计,选用3头年龄相同(3胎次)、体重相近[(500±50)kg]且安装永久性瘤胃瘘管的中国荷斯坦奶牛为试验动物,分别饲喂基础日粮、豆皮糖蜜和麸皮糖蜜日粮。结果表明,豆皮糖蜜组和麸皮糖蜜组瘤胃pH均极显著高于对照组(P0.01),而豆皮糖蜜组和麸皮糖蜜组之间瘤胃pH没有显著差异(P0.05);麸皮糖蜜组瘤胃NH_3-N浓度显著低于对照组(P0.05),但与豆皮糖蜜组没有显著差异(P0.05);各处理间奶牛瘤胃各单个挥发性脂肪酸(volatile fatty acid,VFA,包括乙酸、丙酸、异丁酸、丁酸、异戊酸和戊酸)浓度、总VFA浓度和乙丙比均没有显著差异(P0.05);但与对照组相比,豆皮糖蜜组瘤胃总VFA浓度有降低趋势;而麸皮糖蜜组瘤胃总VFA浓度有提高趋势。结果显示,在奶牛饲料中可用豆皮糖蜜替代15%玉米以及用麸皮糖蜜替代6%玉米和10%麸皮,均具有调控瘤胃稳衡的作用,且麸皮糖蜜替代效果优于豆皮糖蜜。 相似文献
49.
50.
为研究乳酸菌液对仔兔生长性能及对断奶仔兔肠道菌群的影响,试验选用加利福尼亚兔×新西兰白兔二元杂交仔兔400只,随机分成2组,每组5个重复,于15日龄后进行乳酸菌液饮水,以观察仔兔的生长性能和断奶仔兔肠道菌群的影响。结果:饲喂乳酸菌液能显著提高仔兔的生长性能,在30~40日龄、40~70日龄显著高于对照组(P0.05);断奶前后乳酸菌液组乳酸菌的含量显著高于对照组,大肠杆菌数量差异不显著。 相似文献