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541.
Tomomi Ohira Chiaki Murayama Takashi Shimizu Yukinori Yoshimura Naoki Isobe 《Animal Science Journal》2013,84(4):303-309
As stage progresses in the cystic follicle, granulosa cells are lost. We hypothesized that the granulosa and theca interna layers are detached in association with weakened expression of cell adhesion molecules such as cadherin (cell–cell adhesion) and integrin (cell–extracellular matrix adhesion) in cystic follicles. To elucidate this hypothesis, we immunolocalized these molecules in the granulosa and theca interna and compared them between cystic and small healthy follicles. Sections were immunostained with cadherin and integrin β1 antibodies and their localizations were compared. Cadherin‐positive reaction was seen in the cytoplasma of all granulosa cells. No increase in the frequency of cadherin‐positive area in the granulosa layers and the intensity of cadherin immunoreaction in the theca interna was detected in cystic follicles compared with healthy ones. A dense immunoreaction product of integrin β1 was detected in the theca interna in both cystic and healthy follicles. Intensity of integrin β1‐immuno reaction in the granulosa layers and integrin β1‐positive area in the theca interna was significantly lower in the cystic follicle than in the healthy follicles. These results suggest that granulosa and theca interna cells are detached while maintaining the cell–cell adhesion, resulting in the consequent loss of these layers from the cystic follicle. 相似文献
542.
Mariko Nagatani Kayoko Kudo Seiki Yamakawa Toko Ohira Yuko Yamaguchi Shinichiro Ikezaki Isamu Suzuki Tsubasa Saito Toru Hoshiya Kazutoshi Tamura Kazuyuki Uchida 《Journal of toxicologic pathology》2013,26(3):263-273
In order to accurately assess the carcinogenicity of chemicals with regard to rare
tumors such as rat CNS tumors, sufficient information about spontaneous tumors are very
important. This paper presents the data on the type, incidence and detected age of CNS
tumors in F344/DuCrlCrlj (a total of 1363 males and 1363 females) and Crl:CD(SD) rats (a
total of 1650 males and 1705 females) collected from in-house background data-collection
studies and control groups of carcinogenicity studies at our laboratory, together with
those previously reported in F344 and SD rats. The present data on F344/DuCrlCrlj rats
(F344 rats) and Crl:CD(SD) rats (SD rats) clarified the following. (1) The incidences of
all CNS tumors observed in F344 rats were less than 1%. (2) The incidences of malignant
astrocytoma and granular cell tumor were higher in male SD rats than in female SD rats.
(3) The incidences of astrocytoma and granular cell tumor were higher in SD rats than in
F344 rats. (4) Among astrocytoma, oligodendroglioma and granular cell tumor,
oligodendroglioma was detected at the youngest age, followed by astrocytoma, and
ultimately, granular cell tumor developed in both strains. The incidences observed in our
study were almost consistent with those previously reported in F344 and SD rats. 相似文献
543.
544.
Ji‐Seon Yoon Koji Nishifuji Sinpei Ishioroshi Kaori Ide Toshiroh Iwasaki 《Veterinary dermatology》2013,24(1):84-e22
Background – Seborrhoea is a clinical condition resulting in excessive lipid and/or scale on the skin and is a common and important skin disease of dogs. However, there is little information on the skin surface lipid composition of dogs with seborrhoea. Hypothesis/Objectives – To compare skin surface lipid profiles in normal and seborrhoeic shih tzu dogs. Methods – Fourteen client‐owned dogs (seven seborrhoeic and seven normal) were investigated. Lipids in sebaceous glands (SGs) were extracted from homogenized tissues of SG hyperplasia. Surface lipid was collected by tape stripping [stratum corneum (SC)‐enriched fraction] and acetone‐wetted cotton swab (acetone‐extracted fraction). Lipids in SGs, SC‐enriched fractions and acetone‐extracted fractions were evaluated by high‐performance thin‐layer chromatography. Results – Lipids in SGs mainly consisted of cholesterol esters, wax esters and triglycerides, whereas lipids in the SC‐enriched fraction mainly consisted of ceramides. The acetone‐extracted fraction contained a mixture of lipid classes recognized in SG‐ and SC‐enriched fractions. In seborrhoeic dogs, concentrations of wax esters and triglycerides in the acetone‐extracted fraction were significantly higher than in control dogs (P = 0.0285). Amounts of total ceramides (in micrograms) per milligram of SC were not significantly different between the two groups (P = 0.5204). Interestingly, two unknown ceramide fractions, which accounted for 20% of the total ceramides, were recognized exclusively in seborrhoeic dogs. Conclusions and clinical importance – These results provide evidence that the skin surface lipid profiles are altered in shih tzu dogs with seborrhoea. 相似文献
545.
Koji MISUMI Yuri HIRAYAMA Sachiko EGAWA Shoko YAMASHITA Hiroyoshi HOSHI Kei IMAI 《The Journal of reproduction and development》2013,59(6):520-524
This study was conducted to clarify the feasibility of newly developed vitrification
techniques for porcine embryos using the micro volume air cooling (MVAC) method
without direct contact with liquid nitrogen (LN2). Expanded blastocysts
were vitrified in a solution containing 6 M ethylene glycol, 0.6 M trehalose and 2%
(wt/vol) polyethylene glycol in 10% HEPES-buffered PZM-5. The blastocysts were
collected from gilts and vitrified using the new device (MVAC) or a Cryotop (CT).
Blastocysts were stored in LN2 for at least 1 month. After warming,
cryoprotective agents were removed using a single step. Survival of the embryos was
assessed by in vitro culture (Experiment 1) and by embryo transfer
to recipients (Experiment 2). In Experiment 1, the embryos vitrified by the MVAC or
CT and fresh embryos without vitrification (Control) were used. The survival rates of
embryos in the MVAC, CT and Control groups were 88.9% (32/36), 91.7% (33/36) and 100%
(34/34), respectively, after 48 h culture, and the hatching rates of embryos after 48
h incubation were 69.4% (25/36), 63.9% (23/36) and 94.1% (32/34), respectively. In
Experiment 2, 64 vitrified embryos were transferred to 5 recipient gilts, and 8
healthy piglets were produced from 3 recipients in the MVAC group. Similarly, 66
vitrified embryos were transferred to 5 recipient gilts, and 9 healthy piglets were
produced from 2 recipients in the CT group. These results indicated that porcine
expanded blastocysts can be cryopreserved using the MVAC method without potential
pathogen contamination from LN2. 相似文献
546.
547.
Keisuke Tomioka Toshiaki Takehara Hideki Osaki Hiroyuki Sekiguchi Koji Nomiyama Koji Kageyama 《Journal of General Plant Pathology》2013,79(2):162-164
Severe rotting and blight of seedlings of soybean, Glycine max (L.) Merr., were found in Hiroshima Prefecture in the southwest region of Japan in July 2009. A filamentous microorganism, isolated repeatedly from the diseased seedlings and identified as Pythium myriotylum, was demonstrated to be pathogenic to seedlings of soybean. The disease was new in Japan, and we propose to include this oomycete as one of the pathogens causing damping-off of soybean. 相似文献
548.
549.
Physical, chemical, and biological properties of s-allylcysteine, an amino acid derived from garlic. 总被引:5,自引:0,他引:5
Yukihiro Kodera Ayumi Suzuki Osamu Imada Shigeo Kasuga Isao Sumioka Atsushi Kanezawa Nobuo Taru Masanori Fujikawa Shinji Nagae Koji Masamoto Katsuhiko Maeshige Kazuhisa Ono 《Journal of agricultural and food chemistry》2002,50(3):622-632
Physical, chemical, and biological properties of S-allylcysteine (SAC) were investigated. SAC showed stable properties under tested conditions, and its acute/subacute toxicity was very minor in mice and rats (LD(50) value >54.7 mM/kg po; >20 mM/kg ip). The pharmacokinetics of SAC was investigated after oral administration of garlic supplement containing SAC to human volunteers. SAC from garlic consumption was rapidly absorbed from the gastrointestinal tract, however, the half-life and excretion time were more than 10 h and 30 h, respectively. 相似文献
550.
Lu Meng Chao Koji Takayama Yoshitaka Nakanishi Katsumi Hamana Mitsuhiro Takagi Chikara Kubota Toshiyuki Kojima 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(1):95-101
The present experiment aims to examine the efficiency of estrus synchronization using progesterone and equine chorionic gonadotrophin (eCG) and to look at luteal function. During the non-breeding and breeding season, 5 adult female Korean native goats were injected intramuscularly with 2.5 ml of physiological saline as the control. A progesterone impregnated intravaginal sponge was then kept in the same goats for 10 days followed, after a week, by an intramuscular injection of 500 IU eCG. Five adult female Nubian goats were mated with a fertile buck during the non-breeding season. During the non-breeding season 2 of the 5 goats showed a normal estrous cycle (ranging from 18 to 21 days) and 3 a short estrous cycle (ranging from 3 to 6 days). During the breeding season the equivalent figures were 1 and 2. The major axes of the corpus luteum (CL) were measured by means of calipers built into the ultrasonography system, and the concentrations of plasma progesterone (P4) were determined by double antibody radioimmunoassay. The mean major axes of the CL in goats showing the short cycle (6.1 ± 0.5 mm) was significantly smaller than in those showing the normal cycle (8.9 ± 0.5 mm; p < 0.01) and also the value of P4 in goats showing the short cycle (4.2 ± 2.1 ng/ml) was significantly lower than for those showing the normal cycle (10.3 ± 4.3 ng/ml; p < 0.05) at day 3 following ovulation. Three out of 5 Nubian goats became pregnant but only one goat carried to full term. The present experiment indicated that a combination of progesterone and eCG was effective in inducing estrus, although it resulted in a high incidence of short luteal lifespan. The low kidding rate and high incidence of embryonic loss may be due to the instability of the luteal lifespan. 相似文献