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31.
32.
Yasuhiro Inoue Takayuki Matsuura Tatsuji Ohara Koji Azegami 《Journal of General Plant Pathology》2006,72(2):111-118
The entire genome of bacteriophage OP1, lytic for Xanthomonas oryzae pv. oryzae causing bacterial leaf blight of rice, and the partial genomes of related phages were sequenced and analyzed. The OP1 genome comprises double-stranded, 4785-bp long DNA with 51.1% G + C content. Fifty-nine open reading frames (ORFs) were detected.
ORF25 had similarity with the tail fiber gene of phages, whose product is related to host specificity. The ORF25 regions were
amplified from four host-range mutants (OP1h, OP1hC, OP1h2, and OP1h2C) by polymerase chain reaction, and their deduced amino acid sequences were compared. Three mutants (OP1hC, OP1h2, OP1h2C) had duplications of a small domain in the N-terminal portion, although there were slight differences in the position of the duplicated sequences. One mutant OP1h had substituted amino acids in the duplication region. New mutants isolated in the laboratory (OP1hC and OP1h2C from OP1 and OP1h2) acquired the ability to lyse strain N5874 belonging to phagovar (lysotype) C. However, they rapidly lost this lytic ability
when incubated with other phagovars. This loss was always accompanied by a loss of the characteristic repeats, suggesting
that the host range of OP1-related phages changed mainly through duplication and deletion of a small domain in ORF25.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AP008979,
AB214312 to AB214316 相似文献
33.
The timing of parturition was recorded for a total of 56 beef cattle (Japanese Black × Holstein Friesian) on different dietary treatments. The rate of calving during daylight hours in cows night‐fed (18.00 hours) with a roughage diet was significantly higher than that in cows night‐fed with a high concentrate diet (79.2% vs 38.5%, P < 0.05). Subsequently, the vaginal temperature (VT) of these cows was analyzed using a cosinor method. When the feeding schedule was changed from twice daily (08.30 and 15.30 hours) to night feeding, the periodicity, the acrophase and the bathyphase, which were the parameters of the cosine curve, were unstable from the first day of night feeding until after day 6 (P < 0.05). Prior to parturition, the midline‐estimating statistic of rhythm (MESOR) and the amplitude for the cows that were fed a high‐roughage diet at night and that calved at night‐time were lower and larger, respectively, than that for the other treatments (P < 0.01). Based on these results, the time of parturition in most of the beef cows was influenced by feeding time and diet composition. Those cows that calved at night‐time in spite of night feeding had lower vaginal temperatures. 相似文献
34.
We induced various flower morphologies in torenia (Torenia fournieri Lind.) by the application of forchlorfenuron (CPPU). Those morphologies were the combination of four basic morphological changes, the development of serrate petals, incised petals, a paracorolla, and an increased number of floral organs. These morphological changes occurred systematically depending on the floral stage at the time of CPPU application. Serrate petals were induced when CPPU was applied during the stages of corolla development, whereas application at younger stages induced petal incision. The serrate petal margin resulted from preferential proliferation of cells around the vascular bundles, whereas petal incision likely resulted from the lateral outgrowths of petal. A paracorolla was induced at the adaxial petal face when CPPU was applied between the sepal development stage and early corolla development. The paracorolla appears to have arisen from the lateral outgrowths of the stamen. The numbers of stamens, petals, and sepals increased when CPPU was applied at and before the differentiation of sex organs and the corolla. Enlargement of the floral meristem probably caused this increase. Application of N6-benzylaminopurine and zeatin did not induce these morphological changes. 相似文献
35.
Takao Tsukiboshi Yoshiaki Chikuo Yoko Ito Yosuke Matsushita Koji Kageyama 《Journal of General Plant Pathology》2007,73(4):293-296
Root and stem rot with wilt of above ground parts of cultivated chrysanthemums was first found in Ibaraki, Toyama and Kagawa
prefectures, Japan in 2002 and 2003. Pythium species were isolated from the diseased tissues and identified as P. dissotocum, P. oedochilum, P. sylvaticum, P. ultimum var. ultimum and asexual strains of P. helicoides based on their morphologies and sequences of rDNA-ITS region. All the Pythium species were strongly pathogenic to chrysanthemums in pot conditions and were reisolated from the inoculated plants. Because
Pythium root and stem rot of chrysanthemum has never been reported in Japan, we propose that this is a new disease that can
be caused by the five Pythium species. 相似文献
36.
37.
Koji?YoshidaEmail author Ryota?Shibasaki Chika?Takami Chisato?Takenaka Kazukiyo?Yamamoto Takafumi?Tezuka 《Journal of Forest Research》2004,9(3):195-203
In order to clarify the chronic influence of acid fog on the gas exchange rates of momi fir (Abies firma Sieb. et Zucc.) trees, we exposed them to simulated acid fog (pH 3) for 3 years. The composition of the acid fog was similar to that observed in a region where momi fir trees have been declining, and it contained organic acids. We then treated the firs with various additional stresses, such as drought, low temperature, fine root cutting, ozone exposure, soil acidification, nitrogen load, and rhizosphere aluminum stress. Under chronic exposure to acid fog, the momi fir seedlings exhibited a pattern of stomatal behavior whereby they excessively opened in summer and closed in winter. Furthermore, the stomata of these seedlings tended to open during drought stress, and their needles were visibly injured after ozone exposure. The net photosynthesis rates of the seedlings exposed to acid fog were regulated by their stomatal aperture, rather than directly by acid fog. These results suggest that acid fog exposure disturbs the control of stomatal function in the momi fir seedlings. In addition, we found that chronic acid fog exposure suppressed the decrease in net photosynthesis rate, due to its nitrogen load. 相似文献
38.
Antifeedant activity of some flavonoids and their related compounds against the subterranean termiteCoptotermes formosanus Shiraki was examined with nochoice tests and two-choice tests. The activities of these compounds were evaluated in relation to their chemical structures. All flavonoids tested showed antifeedant activity, whereas catechinic acid, possessing no A-ring or pyran ring in the molecule, showed feeding-preference activity. For the structure-activity relations, it was found that compounds containing two hydroxyl groups at C-5 and C-7 in A-rings showed high antifeedant activity. Furthermore, the presence of a carbonyl group at C-4 in the pyran rings of the compounds was necessary for the occurrence of high activity. 3-Hydroxyflavones and 3-hydroxyflavanones with 3,4-dihydroxylated B-rings exhibited higher activity than those with 4-hydroxylated B-rings.Part of this paper was presented at the 49th annual meeting of the Japan Wood Research Society, Tokyo, April 1999 相似文献
39.
The bark of Japanese cedar (Cryptomeria japonica D. Don) was heated in an N2 atmosphere at 300–900C for 3h to investigate the surface properties of the pyrolyzed residuces and their ability to remove trichloroethylene (TCE) from aqueous solutions. The specific surface areas (S
N) and total pore volumes (V
N) of the pyrolyzed barks steadily increased with rising pyrolysis temperature; no significant differences in the average pore diameters (D
N) were observed at higher temperatures (600–900C). The adsorption capacities of the pyrolyzed residues for TCE were determined under batch mode conditions using an aqueous solution containing 500g TCE dm–3. The adsorption data were well fitted to the Freundlich equation. The adsorption capacity of the bark pyrolyzed at 900C was about five times larger than that of commercial activated carbon.This study was presented at the 10th International Symposium on Wood and Pulping Chemistry, Yokohama, June 1999 相似文献
40.
Chikaya Sakai Atok Subiakto Hani Sitti Nuroniah Naoto Kamata Koji Nakamura 《Journal of Forest Research》2002,7(2):73-80
We developed a vegetative propagation system for mass-producing three dipterocarps species,Shorea selanica Bl.,Shorea leprosula Mig., andShorea platyclados Sloot. This system uses fog evaporative cooling inside a greenhouse to reduce the leaf-to-air vapor pressure deficit (leaf-to-air
VPD) inside the propagator, even under high irradiance conditions. This cooling method has no negative influence on medium
conditions such as overwetting. A plastic tent propagator combined with this cooling method was used for vegetative propagation
experiments. In mass-production experiments, the annual rooting percentages from the cuttings were low in the first 2 years
(1997–1998) due to operational problems of the tent propagator (S. selanica, 48–51%;S. leprosula, 56–59%;S. platyclados: 50–63%). A hard cover propagator improved the rooting percentages in the mass-production experiments in 1999 because it
made operations easier (S. selanica, 70%;S. leprosula, 77%;S. platyclados, 77%). This system, which uses a combination of fog evaporative cooling and a hard plastic propagator, should be useful for
the mass propagation of these dipterocarp planting stocks.
This study was carried out under a joint project of the Ministry of Forestry, Indonesia and Komatsu Ltd. The project was supported
by Research Association for Reforestation of Tropical Forest (RETROF) organized by Japanese Forestry Agency. 相似文献