A MACROSCOPIC AND RADIOLOGIC INVESTIGATION OF DENTAL DISEASES OF THE DOG

The type and frequency of dental diseases and disorders were determined in 162 randomly selected dogs available for necropsy. There were 82 males and 80 females ranging in age from seven months to 14 years. There were 150 purebred dogs (50 different breeds) and 12 mongrels. Only four breeds were represented by ten individuals or more: Poodles (Miniature and Toy), German Shepherds, Dachshunds, and Boxers. The oral cavity was examined macroscopically, and missing teeth, dental caries, dental calculus (tartar), and gingival epulides were recorded as to frequency and distribution. After macroscopic examination of the oral cavity, the head of each dog was sawed at the midline, fixed in formalin, and radiographed. The frequency, severity, and distribution of marginal periodontitis, periapical destruction, and root resorption were recorded. The most frequently noted disease was periodontitis, which increased in frequency and severity with increasing age of the dogs. The disease varied markedly among and within different breeds. Small dogs were more often affected with periodontitis than large dogs. Periapical destruction was a common finding. Caries and root resorption were less common. Missing teeth was a frequent disorder regardless of age. Most of the dogs had dental calculus.     

Der M. cucullaris beim Amerikanischen Tapir (Tapirus terrestris, L. 1758)

The reexamination of the branchiogenic muscles of the shoulder shows also in the Lowland Tapir, that the dorsal branch of the Ramus ext. of the N. accessorius innervates the three parts of the M. trapezius (Pars clavicularis, cervicalis and thoracalis), and the ventral branch innervates the two parts of the M. sternocleidomastoideus (Pars sternomandibularis and sternomastoidea). There is no Pars cleidomastoidea in the latter muscle. As common name for all muscles innervated by the Ramus ext. of the N. accessorius, the term M. cucullaris is proposed again.     

Phytochemische Untersuchungen an Pflanzen der einheimischen Flora und des Gaterslebener Sortiments I. Mitteilung

Zusammenfassung 317 Pflanzenarten aus 73 Familien der einheimischen Flora und des Gaterslebener Sortiments wurden nach einem ausführlich beschriebenen Standardverfahren orientierend auf Alkaloide, Saponine, Triterpene und Sterine untersucht.

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Summary 317 plant species from 73 families of the indigenous flora and of the sortiment in Gatersleben have been preliminarily investigated for alkaloids, saponins, triterpenes, and sterols, using a fully described standard procedure.

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m 317 73 - «», «», «» «». .

     

Über die Sterine vonSolanum tuberosum L. Sterine und Triterpenoide. VIII. Mitteilung

Zusammenfassung Aus Blättern der KartoffelpflanzeSolanum tuberosum Aquila wurde außer Cycloartenol (I), 24-Methylen-cycloartanol (II) (?), Lophenol (III), 24-Methylen-(IV) und 24-Äthyliden-lophenol (V) in 0,05%iger Ausbeute (bezogen auf Blatt-Trockengew.) ein Steringemisch isoliert, das nach dem Molekül-Massenspektrum (Ionisierung durch Anlagerung energiearmer negativer Ladungsträger) aus etwa 49% -Sitosterin (VIII), 36% Stigmasterin (IX), 12% Cholesterin (VI) und 3% Campesterin (VII) zusammengesetzt ist. Weiterhin ließen sich die homologen n-Paraffine C₂₅H₅₂ bis C₃₃H₆₈ und iso-Paraffine C₂₉H₆₀ bis C₃₃H₆₈ (Hauptkomponente mit ca. 51% n-Hentriacontan) sowie geringe Mengen höherer aliphatischer Ketone, vor allem Tritriacontanon, gaschromatographisch bzw. molekülmassenspektrographisch nachweisen.

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Summary In addition to cycloartenol (I), 24-methylene-cycloartanol (II) (?), lophenol (III), 24-methylene-(IV) and 24-ethylidene-lophenol (V) a mixture of sterols has been isolated from leaves of the potato plantSolanum tuberosum Aquila (0,05% of the dry weight), which, according to our molecular mass spectrographic investigations employing negative ionization, contains approximately 49% -sitosterol (VIII), 36% stigmasterol (IX), 12% cholesterol (VI), and 3% campesterol (VII). Furthermore the homologous n-alkanes C₂₅H₅₂ to C₃₃H₆₈ and iso-alkanes C₂₉H₆₀ to C₃₃H₆₈ (the main component being n-triacontane with ca. 51%) as well as some higher aliphatic ketones, especially tritriacontanone, have been detected by gas-liquid chromatographic and molecular mass spectrographic methods.

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am ¶rt;au Solanum tuberosum L., , (I), 24- (II) (?), (III), 24-- (IV) 24-- (V) ( 0,05% ). « -» ( ) 49%- (VIII), 36% (IX), 12% (VI) 3% (VII). () « -» - ( _{2552 3368}) - ( _{2960 3368}), 51%-, , .

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VII. Mitteilung: von Ardenne und Mitarb. (1965b).

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Zugleich IV. Mitteilung der Reihe Molekül-Massenspektrographie von Naturstoffen. — III. Mitteilung: von Ardenne und Mitarb. (1965b).

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Forschungsinstitut Manfred von Ardenne, Dresden-Weißer Hirsch.

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Teil der Dissertation von G. Osske, Univ. Halle, 1963.     

Prion protein in sheep urine.

The misfolded form of cellular prion protein (PrP(C)) is the main component of the infectious agent of transmissible spongiform encephalopathies and the validated biomarker for these diseases. The expression of PrP(C) is highest in the central nervous system and has been found in peripheral tissues. Soluble PrP(C) has been detected in cerebrospinal fluid, urine, serum, milk, and seminal plasma. In this study, attempts were made to characterize prion protein in urine samples from normal and scrapie-infected sheep. Urine samples from scrapie-infected sheep and age-matched healthy sheep were collected and analyzed by Western blot following concentration. A protease K-sensitive protein band with a molecular weight of approximately 27-30 kDa was visualized after immunoblotting with anti-PrP monoclonal antibodies to a C-terminal part of PrP(C), but not after immunoblotting with monoclonal antibodies to an N-terminal epitope of PrP(C) or with secondary antibodies only. The amount of PrP(C) in the urine of 49 animals (control group: n = 16; naturally scrapie-infected group: n = 33) was estimated by comparison with known amounts of ovine recombinant PrP in the immunoblot. Background concentration of PrP(C) in urine was found to be 0-0.16 ng/ml (adjusted to the initial nonconcentrated volume of the urine samples). Seven out of 33 naturally scrapie-infected animals had an elevated level (0.3-4.7 ng/ml) of PrP(C) in urine. The origin of PrP(C) in urine and the reason for the increased level of PrP(C) in scrapie-infected sheep urine has yet to be explored.