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51.
Masayasu Saruta Akio Kikuchi Akinori Okabe Takahide Sasaya 《Journal of General Plant Pathology》2005,71(6):431-435
Coat protein sequences of two isolates in strain A2 and five isolates in strain D of Soybean mosaic virus (SMV), which caused a recent mosaic outbreak in soybeans (cv. Sachiyutaka) in Chugoku and Shikoku in Japan, were compared
to published data on 15 other Asian-origin isolates. Sequence comparison and cluster analysis showed that SMV isolates of
strain A2 from these districts were closely related, as were those of strain D, but strains A2 and D were not. Thus, the two strains may have different origins and be carried through seed transmission. 相似文献
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Tetsuo Tamura Kazunori Inoue Hideto Nishiki Masafumi Sakata Makoto Seki Teruaki Koga Yoshihiro Ookubo Kazuhiro Akutsu Say Sato Kouichi Saitou Hikari Shinohara Terumi Kuraisi Hiroshi Kajikawa Mitsunori Kurihara 《Animal Science Journal》2019,90(1):62-70
We evaluated the influence on milk production of feeding early lactation cows a diet that included 14.5% crude protein (CP) and that did not meet methionine (Met) requirements or that met them by supplying rumen‐protected Met (RPMet). Thirty‐nine multiparous Holstein cows were allocated into two groups. For 15 weeks after calving, each group was fed one of the two total mixed rations, Control (n = 20) or Treatment (n = 19). The Treatment group received added RPMet at 0.034% (8 g/day) of the Control diet on dry matter basis. The adequacies of Met for the Control and Treatment groups were 96% and 106%, respectively, and for other amino acids, >110%. The CP level (14.5%) was 1 percentage point lower than that recommended by the Japanese Feeding Standard (2006). No between‐group differences were found in milk yield (40 kg/day), milk composition, plasma profile, rumen fermentation, nitrogen balance, or cow health. Met intake and the amount of rumen‐undegradable feed Met were higher in the Treatment group (p < 0.05). Microbial Met and total metabolizable Met did not differ between groups. Supplying RPMet in a 14.5% CP diet during early lactation did not dramatically affect milk production, because the amount of total metabolizable Met was unchanged. 相似文献
56.
Hiroyuki Kaneko Kazuhiro Kikuchi Nguyen Thi Men Junko Noguchi 《Animal Science Journal》2019,90(3):344-352
Ovarian xenografting makes it possible to obtain oocytes with fertilization ability from immature pigs of Western breeds. In this study, we applied these methods to the Meishan, an indigenous Chinese pig breed, and investigated the developmental competence of oocytes grown in their neonatal tissue after grafting into nude mice. First, mice harboring neonatal ovarian tissue were infused with follicle stimulating hormone (FSH) (62.5 U/ml) for 13 days starting at 10, 30, and 60 days after vaginal opening (D10‐, D30‐, and D60‐FSH groups, respectively). Development of antral follicles and their oocytes was most enhanced in the D60‐FSH group. For the next step, we examined the in vitro maturation ability of the oocytes recovered from host mice after infusion with FSH at a dose of 62.5 U/ml or 125 U/ml (FSH‐62.5 or ‐125 group) for 13 days starting at 60 days after vaginal opening. Many more oocytes with maturation ability were obtained from the FSH‐125 group. The FSH‐125 mature oocytes were fertilized in vitro, as shown by formation of male and female pronuclei, but did not reach the blastocyst stage. These results indicate that Meishan neonatal ovaries are able to produce oocytes with fertilization ability after being grafted into nude mice. 相似文献
57.
Thanh‐Van Nguyen Lanh Thi Kim Do Tamas Somfai Takeshige Otoi Masayasu Taniguchi Kazuhiro Kikuchi 《Animal Science Journal》2019,90(12):1530-1536
Chlorogenic acid (CGA) is known to protect oocytes from oxidative stress. Here we investigated the effects of CGA on porcine oocyte maturation under heat stress and subsequent embryonic development after parthenogenetic activation. For in vitro maturation (IVM) at 41.0°C (hyperthermic condition), supplementation of the maturation medium with 50 μM CGA significantly improved the percentage of matured oocytes and reduced the rate of apoptosis relative to oocytes matured without CGA (p < .05). CGA treatment of oocytes during IVM under hyperthermia tended to increase (p < .1) percentage of blastocyst formation after parthenogenesis and significantly increased (p < .05) the total cell number per blastocyst relative to oocytes matured without CGA. For IVM at 38.5°C (isothermic condition), CGA significantly improved the rate of blastocyst development compared with oocytes matured without CGA (p < .05), but did not affect oocyte maturation, apoptosis rate or the number of cells per embryo. Omission of all antioxidants from the IVM medium significantly reduced the rate of oocyte maturation, but the rate was restored upon addition of CGA. These results demonstrate that CGA is a potent antioxidant that protects porcine oocytes from the negative effects of heat stress, thus reducing the frequency of apoptosis and improving the quality of embryos. 相似文献
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Shin Kato Yoshitake Takada Satoshi Shimamura Kaori Hirata Takashi Sayama Fumio Taguchi-Shiobara Masao Ishimoto Akio Kikuchi Takeshi Nishio 《Breeding Science》2016,66(2):319-327
Resistance to soybean mosaic virus (SMV) is imperative for soybean (Glycine max (L.) Merr.) production in the Tohoku region. Molecular markers for SMV resistance were previously reported for U.S. SMV strains, but they cannot be applied because of the differences in strain classification between Japan and the U.S. A U.S. variety ‘Harosoy’ has been used mainly as a donor of resistance to SMV strains C and D in a Japanese breeding program, resulting in resistant varieties such as ‘Fukuibuki.’ Because ‘Harosoy’ harbors the Rsv3 gene conferring resistance to the virulent SMV strain groups, G5 through G7, it appears that the Rsv3 gene confers resistance to strains C and D. In this study, we introduced resistance to the two strains from ‘Fukuibuki’ into a leading variety ‘Ohsuzu’ by recurrent backcrossing with marker-assisted selection. All lines selected with markers near Rsv3 showed resistance to the strains, suggesting that the Rsv3 locus is responsible for the resistance. Three years of trials showed that one of the breeding lines, ‘Tohoku 169,’ was equivalent to ‘Ohsuzu’ with respect to agricultural characteristics such as seed size, maturity date, and seed yield, except for the SMV resistance. 相似文献
60.
Junichi Kikuchi Souichirou Iida Tamio Akema Kazuyoshi Futai 《Journal of Forest Research》1996,1(1):37-40
The process of decrease in seedlings of Japanese red pine (Pinus densiflora Sieb.et Zucc.) and the effect of litter and humus layers on the mortality were studied in their stand and adjacent bare land. After
the rainy season, only small number of individuals died in the bare land but more seedlings died in the forest. The number
of seedlings killed by drought was greater in the forest than in the bare land. The population of naturally occurring current
year seedlings was larger in the forest than in the bare land, but that of 1- to 5-year-old seedlings was smaller. Severe
summer drought exceeding the permanent wilting point was observed only in the forest F-H horizon where greater part of seedling
roots distributed, this may be one of major mortality factors. Inhibitory substances to seedling growth in fresh fallen pine
needles were also suggested. 相似文献