Dietary supplementation effects of Pediococcus acidilactici as probiotic on growth performance,digestive enzyme activities and immunity response in zebrafish (Danio rerio)

This study was carried out to assess the effects of Pediococcus acidilactici on zebrafish (Danio rerio). Different levels of P. acidilactici including 0, 1 × 10⁶, 2 × 10⁶, 4 × 10⁶ and 8 × 10⁶ colony‐forming unit per g of the diet (cfug^?1) were examined in fish with 120 ± 10 mg weight for 60 days in a completely randomized design. The results showed that the best growth indices were recorded in group 4 × 10⁶ cfug^?1 (p < 0.05). The highest number of total viable count and lactic acid bacteria of intestine were found in group 4 × 10⁶ cfug^?1 (p < 0.05). The maximum activity of digestive enzymes including amylase, lipase, protease and alkaline phosphatase was observed in group 4 × 10⁶ cfug^?1. The highest activity for superoxide dismutase was recorded in group 4 × 10⁶ cfug^?1 while catalase, glutathione peroxidase and glutathione reductase showed the highest activity in group 8 × 10⁶ cfug^?1. The most growth inhibition zone of Aeromonas hydrophylla, Flavobacterium columnare, Vibrio anguillarum and Edwardsiella tarda was found in group 4 × 10⁶ cfug^‐1 (p < 0.05). Therefore, P. acidilactici as a probiotic improved growth and immunity of the zebrafish and could be used by zebrafish farmers.     

Beneficial effects of Bacillus subtilis on water quality,growth, immune responses,endotoxemia and protection against lipopolysaccharide‐induced damages in Oreochromis niloticus under biofloc technology system

The present research explored the effects of Bacillus subtilis on water quality, growth, immune responses, endotoxemia and protection against lipopolysaccharide (LPS) damages in Nile tilapia Oreochromis niloticus under biofloc system. B. subtilis was added at 0, 1, 2, 3 and 4 grams (1.19 × 10⁸ CFU/g) per kg of basal diet, named T1 (control), T2, T3, T4 and T5, respectively, and fed to fish (14.82 ± 0.42 g) for 50 days. The concentrations of TAN, NO₂ and NO₃ were significantly reduced, and fish fed probiotics displayed significantly better growth performances versus the control, concomitantly with significantly enhanced activities of digestive enzymes. They also showed significantly declined serum glucose and cholesterol vice versa significantly improved immune responses (total protein, albumin, globulin, lysozyme, alternative complement, protease, immunoglobulins, alkaline phosphatase and respiratory burst), antioxidant capacity (superoxide dismutase, total antioxidant capacity, malondialdehyde) and skin mucus parameters (total protein, lysozyme, alternative complement, protease, immunoglobulins). Meanwhile, significantly lower endotoxin (LPS) concentrations were detected in the intestines and serum of fish fed probiotics. LPS challenge induced profound oxidative stress and impaired immune responses. Interestingly, probiotic alleviated LPS‐induced damages and restored mentioned parameters. In conclusion, B. subtilis effectively enhanced fish production, immunity and protection against LPS‐induced damages in tilapia under biofloc system.     

Hematology of great sturgeon (<Emphasis Type="Italic">Huso huso</Emphasis> Linnaeus, 1758) juvenile exposed to brackish water environment

The effect of environmental salinity on hematological parameters of great sturgeon Huso huso juveniles was studied. Five-month-old juveniles (mean body weight 28.3 ± 2.1 g) were subjected to 0, 3, 6, 9, and 12 ppt salinities. The hematological parameters were assessed after a period of 20 days rearing at these salinities. After transfer from fresh water to brackish water, red blood cells, hematocrit, haemoglobin and mean corpuscular haemoglobin decreased, but mean corpuscular volume increased. Mean corpuscular haemoglobin concentration, white blood cells, monocyte counts, and eosinophil counts showed no significant variations with increase in environmental salinity. An increase was found in lymphocyte counts according to the increase of salinity from 0 to 12 ppt, while the fresh water control group maintained basal levels. Decrease in neutrophil counts was observed in great sturgeon with increase in environmental salinity. These data show significant effect of salinity on the blood parameters of great sturgeon.     

Slope gradient and shape effects on soil profiles in the northern mountainous forests of Iran

In order to evaluate the variability of the soil profiles at two shapes (concave and convex) and five positions (summit, shoulder, back slope, footslope and toeslope) of a slope, a study of a virgin area was made in a Beech stand of mountain forests, northern Iran. Across the slope positions, the soil profiles demonstrated significant changes due to topography for two shape slopes. The solum depth of the convex slope was higher than the concave one in all five positions, and it decreased from the summit to shoulder and increased from the mid to lower slope positions for both convex and concave slopes. The thin solum at the upper positions and concave slope demonstrated that pedogenetic development is least at upper slope positions and concave slope where leaching and biomass productivity are less than at lower slopes and concave slope. A large decrease in the thickness of O and A horizons from the summit to back slope was noted for both concave and convex slopes, but it increased from back slope toward down slope for both of them. The average thickness of B horizons increased from summit to down slopes in the case of the concave slope, but in the case of convex slope it decreased from summit to shoulder and afterwards it increased to the down slope. The thicknesses of the different horizons varied in part in the different positions and shape slopes because they had different plant species cover and soil features, which were related to topography.     

Effects of partial substitution of dietary fish meal by fermented soybean meal on growth performance,amino acid and biochemical parameters of juvenile black sea bream Acanthopagrus schlegeli

A feeding trial was conducted to evaluate the effect of replacing fish meal protein with fermented soybean meal (FSM) on the growth performance, feed utilization, amino acid profile, body composition, morphological parameters, activity of antioxidant and digestive enzymes of black sea bream (Acanthopagrus schlegeli) juvenile. Five isonitrogenic and isolipidic diets were prepared with levels of 0 (control), 80, 160, 240 and 320 g kg^?1 FSM. Triplicate groups (40 fish per tank) of juvenile black sea bream with initial weight of 1.17 ± 0.04 g were hand‐fed to visual satiation at three meals per day for 8 weeks. The fish fed diets containing different levels of FSM had no significant differences regarding survival and specific growth rate compared with control group. Feed and protein efficiency ratios of fish fed diet containing 320 g kg^?1 FSM were significantly lower than those of control group. Daily feed intake and daily protein intake of fish fed diet containing 240–320 g kg^?1 were significantly higher than those of control group. Hepatosomatic index and condition factor of fish were not affected by different dietary FSM level. Fish fed diets containing 240–320 g kg^?1 FSM had significantly higher visceral somatic index than control group. Whole body proximate and amino acid compositions of fish were not affected by dietary FSM level. The activity of digestive enzymes in the intestine was not affected by dietary FSM level. The activity of glutathione peroxidase in liver was significantly higher for fish fed the diet containing 160 g kg^?1 FSM compared with control group. This study showed that up to 40% fish meal in the diets of juvenile black sea bream could be replaced by fermented soybean meal with supplementation of methionine, lysine and taurine.     

Goal programming approach for sustainable forest management （case study in Iranian Caspian forests）

We used a goal programming technique to determine the optimal harvest volume for the Iranian Caspian forest. We collected data including volume, growth, wood price at forest roadside, and variable harvesting costs. The allometric method was used to quantify seques- trated carbon. Regression analysis was used to derive growth models. Expected mean price was estimated using wood price and variable harvesting costs. Questionnaire was used to determine the constraints and the equation coefficients of the goal programming model. The optimal volume was determined using the goal programming method according to multipurpose forest management. LINGO software was used for analysis. Results indicated that the optimum volumes of species were 250.25 m3.ha-1 for beech, 59 m3.ha-1 for hornbeam, 73 m3.ha-1 for oak, 41 m3.ha-1 for alder, and 32 m3.ha-1 for other species. The total optimum volume is 455.25 m3.ha-1.     

Forest Fire Risk Zone Modeling Using Logistic Regression and GIS: An Iranian Case Study

Forest fires are an important environmental concern worldwide, affecting the soil, forests and human lives. During the process of burning, soil nutrients are depleted and the soil is subsequently more vulnerable to erosion. Nowadays it is necessary to identify the factors influencing the occurrence of fire and fire hazard areas, in order to minimize the frequency of fire and avert damage. Logistic regression was used to study the forest fire risk and identify the most influential factors in the occurrence of forest fires. Climatic variables (temperature and annual precipitation), human factors (distance from streams and farmland) and physiography (land slope and elevation) were considered and their correlation with the occurrence of fires investigated. Results of model validation and sensitivity of various areas to fire were examined with the ROC coefficient and Hosmer–Lemeshow test. The estimated coefficients for the independent variables indicated that the probability of occurrence of fire is negatively related to land slope, site elevation and distance from farmlands, but is positively related to amount of annual precipitation.     

Autosomal and sex-linked (co)variance components and genetic parameters for growth traits of Iranian Zandi sheep

Tropical Animal Health and Production - Separation of autosomal and sex-linked direct additive genetic effects has significant role in sheep breeding programs. Hence, this study was conducted to...     

Identifying superior rainfed barley genotypes in farmers’ fields using participatory varietal selection

This study was carried out to identify superior barley genotypes for the rainfed areas of western Iran using a participatory varietal selection (PVS) approach. Three field experiments were conducted in two randomly selected farmers’ fields and in one rainfed research station in the 2006–07 cropping season with 69 genotypes (including one local and one improved check). Several univariate and multivariate methods were used to analyze qualitative (farmers’ scores) and quantitative (grain yield) data. Individual farmers’ scores in each village were positively correlated, indicating that the farmers tended to discriminate genotypes in similar fashion, although the genotypes actually selected by farmers were different in the two villages. In recent years, a greater number of farmers in western Iran preferred the improved variety (Sararood-1) over the local barley (Mahali), while in this project the farmers preferred the new genotypes over the two checks. This was also verified by the quantitative data showing that the checks were outyielded by the new genotypes. Farmers were efficient in identifying the best genotypes for their specific environment, as shown by biplot analysis, indicating their competence in selection. The genotypes selected by the breeder and farmers were almost similar but some differences existed. In conclusion, PVS is a powerful way to involve farmers for selecting and testing new cultivars that are adapted to their needs, systems and environments.     

Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)?

Veterinary Research Communications - Industrial chemical solutions are widely used as a method to disinfection of aquaculture water and environments. The aim of the present study was to evaluate...