Virulence factors of Escherichia cofi from cellulitis or colisepticemia lesions in chickens

This study was designed to compare virulence factors of cellulitis-derived Escherichia coli to colisepticemic E. coli in order to clarify whether E. coli associated with cellulitis comprise a unique subset of pathogenic E. coli. Isolates were tested for serotype, capsule, aerobactin production, colicin production, the presence of the iss gene, and serum resistance. Untypable isolates made up the greatest percentage of each group. Serotypes O2 and O78 were the most commonly identified among both groups of isolates. No statistical differences in the distribution of aerobactin or colicin production, capsule, or iss gene were observed between groups. Cluster analysis showed that 90% of the E. coli isolates had greater than 42% livability in serum-resistance tests. No separation of colisepticemic vs. cellulitis E. coli isolates was observed on the basis of SR. Colicin production by E. coli was highly correlated with serum resistance (P = 0.0029). These data suggest that cellulitis E. coli have virulence traits similar to those of colisepticemic E. coli.     

Identification of potent odorants in different cultivars of snake fruit [Salacca zalacca (Gaert.) Voss] using gas chromatography-olfactometry

Three cultivars of snake fruits, Pondoh Hitam, Pondoh Super, and Gading, were freshly extracted using liquid-liquid extraction. The aroma compounds of the three samples were analyzed by GC-MS and GC-olfactometry using the nasal impact frequency (NIF) method. A total of 24 odor-active compounds were associated with the aroma of snake fruit. Methyl 3-methylpentanoate was regarded as the character impact odorant of typical snake fruit aroma. 2-Methylbutanoic acid, 3-methylpentanoic acid, and an unknown odorant with very high intensity were found to be responsible for the snake fruit's sweaty odor. Other odorants including methyl 3-methyl-2-butenoate (overripe fruity, ethereal), methyl 3-methyl-2-pentenoate (ethereal, strong green, woody), and 2,5-dimethyl-4-hydroxy-3[2]-furanone (caramel, sweet, cotton candy-like) contribute to the overall aroma of snake fruit. Methyl dihydrojasmonate and isoeugenol, which also have odor impact, were identified for the first time as snake fruit volatiles. The main differences between the aroma of Pondoh and Gading cultivars could be attributed to the olfactory attributes (metallic, chemical, rubbery, strong green, and woody), which were perceived by most of the panelists in the Pondoh samples but were not detected in the Gading samples. This work is a prerequisite for effective selection of salak genotypes with optimal aroma profiles for high consumer acceptance.     

Gene introgression in assessing fitness costs associated with phosphine resistance in the rusty grain beetle

Journal of Pest Science - The current study investigates the fitness cost associated with phosphine resistance in the rusty grain beetle, Cryptolestes ferrugineus (Stephens), a problematic pest in...     

Aggregation-based crystal growth and microstructure development in natural iron oxyhydroxide biomineralization products

Crystals are generally considered to grow by attachment of ions to inorganic surfaces or organic templates. High-resolution transmission electron microscopy of biomineralization products of iron-oxidizing bacteria revealed an alternative coarsening mechanism in which adjacent 2- to 3-nanometer particles aggregate and rotate so their structures adopt parallel orientations in three dimensions. Crystal growth is accomplished by eliminating water molecules at interfaces and forming iron-oxygen bonds. Self-assembly occurs at multiple sites, leading to a coarser, polycrystalline material. Point defects (from surface-adsorbed impurities), dislocations, and slabs of structurally distinct material are created as a consequence of this growth mechanism and can dramatically impact subsequent reactivity.     

Diverse plasma populations and structures in Jupiter's magnetotail

Jupiter's magnetotail is the largest cohesive structure in the solar system and marks the loss of vast numbers of heavy ions from the Jupiter system. The New Horizons spacecraft traversed the magnetotail to distances exceeding 2500 jovian radii (R(J)) and revealed a remarkable diversity of plasma populations and structures throughout its length. Ions evolve from a hot plasma disk distribution at approximately 100 R(J) to slower, persistent flows down the tail that become increasingly variable in flux and mean energy. The plasma is highly structured-exhibiting sharp breaks, smooth variations, and apparent plasmoids-and contains ions from both Io and Jupiter's ionosphere with intense bursts of H(+) and H(+)(3). Quasi-periodic changes were seen in flux at approximately 450 and approximately 1500 R(J) with a 10-hour period. Other variations in flow speed at approximately 600 to 1000 R(J) with a 3- to 4-day period may be attributable to plasmoids moving down the tail.     

Life history traits and food supplementation affect productivity in a translocated population of the endangered Hihi (Stitchbird, Notiomystis cincta)

We studied the effect of food supplementation, female age and clutch order on productivity in a translocated species. Food supplementation increased clutch size from 3.9 to 4.4 (average) eggs per nesting attempt, and more than doubled fledging and recruitment success. Supplemented females started a second clutch 9 days sooner after fledging first clutch chicks than unfed females. During second clutches, supplemented females incubated the eggs for a shorter period of time (15.2 days vs. 16.8). Older females laid larger clutches (4.6 vs. 3.7 eggs) than yearlings and incubated second clutch eggs for a shorter period (15.4 vs. 16.6 days). Females laid more eggs in first clutches (4.2 vs. 3.8 eggs), and those eggs took approximately 30% longer to lay than eggs in second clutches. The successful maintenance of hihi populations on the available islands may be dependent on the permanent provision of supplemental food at nest sites.     

Phosphorsäurewirkung in Komplexdüngern

In Inkubationsversuchen wurde das Mineralisierungsverhalten von Ernte‐und Wurzelrückständen (EWR) der auch als Industriepflanzen verwendeten Pflanzenarten Winterraps, Sonnenblume, Öllein, Miscanthus und Sachalinknöterich im Vergleich zu Winterweizen in Abhängigkeit vom Vegetationsstadium und der Bodentemperatur untersucht. Zwischen den Pflanzenarten, ihren Teilen und den Vegetationsstadien bestanden teilweise signifikante Unterschiede in der Mineralisierung. Von dem Pflanzenmaterial in der generativen Phase verblieben nach einer Inkubation von 70 Tagen unter optimalen Bedingungen (25°C, 60% WK_max) 55–70% der eingebrachten C‐Menge im Boden. Intermittierende Temperaturen (Wechsel 10 °C/25 °C) bewirkten eine geringere Mineralisierung, nur 28–34% des Pflanzenmaterials wurden umgesetzt.     

Foliar Iron Fertilization: A Critical Review

Application of foliar iron (Fe) sprays is a common means of correcting Fe deficiency of agricultural crops. However, variable plant responses to iron sprays, ranging from no effect to defoliation, have often been described in the Fe-fertilization literature. Knowledge is still limited concerning the mechanisms of penetration of a leaf-applied, Fe-containing solution and the role of Fe in the leaf. The complex and multi-disciplinary character of the factors determining the effects of Fe sprays hinder the development of suitable foliar fertilization strategies, applicable under variable local conditions and for different plant types. This review describes some key factors involved on the process of penetration of a leaf-applied, Fe-containing solution before briefly analyzing the available foliar Fe-fertilization literature. Iron chemistry, leaf penetration, and plant-nutrition principles will be merged with the aim of clarifying the constraints, opportunities, and future perspectives of foliar Fe sprays to cure plant Fe deficiency.     

Relationship between survival and yield related traits in Solanum pimpinellifolium under salt stress

A subset of the Solanum pimpinellifolium collection maintained by AVRDC—The World Vegetable Center, Taiwan was evaluated to assess effects of salt stress on physiological traits and yield-related traits with the aim of identifying potential S. pimpinellifolium accessions useful for salt tolerance breeding in tomato. We undertook a comparative analysis of yield and plant survival traits under normal and salt stress conditions to obtain a first indication of the crucial traits associated with salt tolerance in S. pimpinellifolium. Although most traits of S. pimpinellifolium accessions showed a similar percent decrease in mean under salt stress compared with the cultivated checks, the former exhibited a wide range for all traits, suggesting great genetic diversity that can be exploited for the identification of salt tolerant genotypes. Genetic variability for yield and survival traits under salt stress was quantitative with low to moderate heritability. Results of correlation and path coefficient analysis revealed no correlation between any of the physiological traits with yield-related traits indicating that the ability to survive and yield under salt stress are two independent sets of traits in S. pimpinellifolium. Results of the path analysis along with heritability and genetic advance showed that shoot dry weight and K/Na ratio are the two most critical component traits for survival, while fruit number is critical for yield per plant. The large S. pimpinellifolium panel evaluated in this study revealed five genotypes possessing better survival traits, seven genotypes with good yield traits, and two genotypes combining both superior survival and yield traits under salt stress.