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Sakaguchi K Iima H Hirayama K Okamoto M Matsuda K Miyasho T Kasamatsu M Hasegawa K Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1217-1220
We report an atypical mycobacterial infection in an Indian flap-shelled turtle, Lissemys punctata punctata, that died in an aquarium in Japan. At necropsy, the turtle showed multiple white nodules on the capsular surface and parenchyma of various organs such as the liver, spleen, intestine, and lung. Histologically, granulomatous inflammation surrounding a central zone of necrosis was observed. Sections stained by the Ziehl-Neelsen method revealed numerous acid-fast bacilli in the cytoplasm of macrophages and in the central area of necrosis. The organisms were identified as a mycobacterial species by PCR and nucleotide sequence analysis and revealed 98-100% homology to M. ulcerans. This is, to our knowledge, the first report of mycobacteriosis due to M. ulcerans in a turtle. 相似文献
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Matsuda K Kobayashi S Yamashita M Hirayama K Kadosawa T Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):479-481
Sarcomatous proliferation of spindle cells was present in the mammary gland and many metastatic sites in a 10-year-old female domestic cat with tubulopapillary carcinoma in the mammary gland. Transition from neoplastic tubular structures to spindle cells in the primary site and fascicular proliferation of the spindle cells with or without coexistance of tubulopapillary carcinoma in the primary and metastatic sites were observed. Most of spindle cells were positive for cytokeratin CAM5.2 as well as the normal luminal epithelium but not the myoepithelium. From these results, this case was diagnosed as tubulopapillary carcinoma with spindle cell metaplasia and it was clarified that neoplastic luminal epithelial cells can transform to sarcomatous appearence. 相似文献
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根癌农杆菌介导的转录因子DREB1A基因在地被菊花中的遗传转化 总被引:11,自引:0,他引:11
以地被菊花(Dendranthema grandiflomm cv.White Snow)无菌苗叶片为外植体,在附加不同浓度植物生长调节剂的MS基础培养基上诱导培养,通过器官直接发生途径获得再生植株。结果表明,2.0mg/L 6-BA和0.2mg/L NAA组合可获得93.8%的再生芽。将含拟南芥(Arabidopsis thaliana)逆境诱导转录因子DREB1A基因的植物表达载体pBI-DREB1A导入根癌农杆菌(Agrobacterium tumefaciens)LBA4404,农杆菌介导法遗传转化地被菊花White Snow叶盘,经PCR和PCR—Southem检测,DREB1A基因已整合到转化植株的基因组中。遗传转化过程中,预培养2d后,将OD600=0.5~0.7的根癌农杆菌稀释30倍后侵染叶盘10min,再共培养2d,有利于获得最高遗传转化效率。 相似文献
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Masahiko Nakagawa Jun Hamatsu Makoto Kuromaru Kazuhito Kita Kazuko Uchiyama 《Journal of Forest Research》2008,13(4):255-260
The efficiency of planting a hybrid larch F1 at two planting densities and of using seedlings of hybrid larch F1 or rooted cuttings of Gream, the registered variety, was investigated. Significantly more time was required to move from
one planting spot to the next in plots with 625 seedlings/ha than in plots with 1,333 seedlings/ha, but there was no significant
difference between seedlings and rooted cuttings. Approximately 18% more time was needed to plant a single planting stock
at the low planting density than at the high density. The need to prune roots or enlarge a planting hole occurred more often
for rooted cuttings than for seedlings, and 17% more time was needed to plant rooted cuttings of Gream than seedlings. 相似文献
25.
Wagner Rodrigo de Souza Nelson Geraldo de Oliveira Felipe Vinecky Ana Paula Ribeiro Marcos Fernando Basso Raphael Augusto das Chagas Noqueli Casari Brbara Andrade Dias Brito da Cunha Karoline Estefani Duarte Thaís Ribeiro Santiago Polyana Kelly Martins Carlos Eduardo Aucique‐Perez Sílvio Carlos Cristofoletti Júnior Alexandre Lima Nepomuceno Carlos Antnio Ferreira de Sousa Adilson Kenji Kobayashi Kazuo Nakashima Kazuko Yamaguchi‐Shinozaki Hugo Bruno Correa Molinari 《Journal of Agronomy and Crop Science》2019,205(6):545-553
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Takaaki Fujimoto Hisashi Akutsu Kazuhito Kita Kazuko Uchiyama Makoto Kuromaru Kazuyuki Oda 《Journal of Wood Science》2006,52(2):101-106
Age trends in variance components and heritability were estimated from the spiral grain angle of rings 2–25, as counted from
the pith in hybrid larch (Larix gmelinii var. japonica × Larix kaempferi) F1. Age-age genetic and phenotypic correlations and the optimum selection age for spiral grain were also calculated. Wood samples
were collected from 95 29-year-old trees belonging to 19 full-sib families in a progeny test plantation in Hokkaido, northern
Japan. Spiral grain angle data were obtained by the splitting method. Mean grain angles at growth rings used for the analyses
were calculated as the arithmetic mean of angles up to respective rings. Generally, the additive genetic variance for mean
grain angle decreased with increasing ring number. Although dominance variance was comparatively high near the pith, it decreased
to zero in subsequent growth rings. Highest heritability estimates of mean grain angle occurred at ring 4 and then declined
with age, ranging from 0.45 down to 0.20. Age-age phenotypic correlations were higher than genetic correlations, especially
those involving early growth rings. Optimum selection ages for spiral grain based on genetic and phenotypic correlations were
estimated as 3 and 4 years (cambial age), respectively, in which maximum gain efficiency per year were obtained. 相似文献
28.
Masato?YoshidaEmail author Masayo?Ikawa Kazuko?Kaneda Takashi?Okuyama 《Journal of Wood Science》2003,49(6):475-478
The tangential strain on the inner bark surface of Fagus crenata sapling stems was continuously measured using strain gauges. The total strain increased daily, increasing at night and decreasing during the day. When tension wood was induced by artificial inclination, the strain increased more on the upper side than on the lower side; and the increment in the strain at night was larger on the upper side than on the lower. The change in tangential strain on the inner bark surface arose from changes in the water content and the volume of differentiating cells. Differentiating tension wood fibers appear to contain more water and to expand more at night than differentiating normal wood fibers. We can determine whether tension wood is formed from the tangential strain during growth. 相似文献
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Sho FUKUI Yoshifumi ENDO Kazuko HIRAYAMA Hiroyuki TANIYAMA Tsuyoshi KADOSAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):747-751
Simultaneous removal of bilateral thyroid tumors was performed while preserving the
parathyroid gland in six dogs. At least one external parathyroid gland was identified in
all dogs. In five cases, the external parathyroid gland and its blood supply were
preserved intact. In one dog, the vessels supplying the external parathyroid gland had
been invaded by the tumor, and the gland was thus removed and reimplanted into the
sternohyoid muscle. That dog required postoperative treatment with oral calcium gluconate
and vitamin D3. Local tumor recurrence was not observed in any of the cases.
The mean survival time was 920 days. We found that the external parathyroid gland could be
identified and preserved in most dogs undergoing total thyroidectomy. 相似文献