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71.
Genome size (C value) is an important index for phylogenetic studies. Haliotidae (abalones) includes many species widely distributed throughout the world??s oceans, which makes this family interesting for phylogenetic studies. To examine Haliotidae phylogeny, we determined the C value and adenine and thymine base pair content (AT?%) of Haliotis discus hannai and H.?diversicolor aquatilis by using flow cytometry. The C values of H.?discus hannai and H.?diversicolor aquatilis were 1.84 and 1.45?pg, with AT?% of 62.3 and 66.3?%, respectively. These data represent the first report of abalones classified as Pacific Northwest (H.?discus hannai) and Indo-Pacific (H.?diversicolor aquatilis) groups, and provides new validation for previous theories related to Haliotidae phylogeny. 相似文献
72.
Huang YQ Morimoto K Hosoda K Yoshimura Y Isobe N 《Veterinary immunology and immunopathology》2012,145(1-2):499-504
Lingual antimicrobial peptide (LAP), one of the β-defensins in bovines, and lactoferrin (LF) are synthesized in mammary epithelium and have bactericidal and bacteriostatic functions. However, it is not known whether they have similar expression patterns. Therefore, the present study was undertaken to compare (1) immunolocalization of LAP and LF in the mammary gland and (2) changes in concentration of these two components in milk after lipopolysaccharide (LPS) challenge. Bovine mammary tissues without LPS challenge were collected and their sections were immunostained with antibodies to LAP or LF. Milk from our previous study was collected every hour up to 12h and twice daily from d 1 to 7 after LPS challenge (the day of infusion was considered as d 0). These milk samples were measured for LAP but not LF in our previous report. Therefore, concentration of LF was measured by enzyme immunoassay in the present study. Epithelial cells of some alveoli showed immunopositive reaction for LF, but negative for LAP. Conversely, some alveoli were LAP positive in their epithelial cells but LF negative. Many alveoli had immunoreactions for neither LAP nor LF. The concentration of LAP in milk was elevated significantly at 3h after LPS infusion compared with pre-infusion values and remained at a high level until 12h. However, LF concentration in milk remained low at d 0 and increased at d 2. These results suggest that LAP and LF were mostly differentially localized in the alveolar epithelium in mammary glands. The different spatial expressions between them may be associated with their different temporal expression mechanisms. 相似文献
73.
Akhil Ranjan Baruah Noriko Ishigo-Oka Mieko Adachi Yasuyo Oguma Yoshiro Tokizono Kazumitsu Onishi Yoshio Sano 《Euphytica》2009,165(3):459-470
The present study was conducted to understand the pattern of variation and the genetic bases for cold tolerance at the early
growth stage in Asian rice. The genetic variation was investigated at the germination, plumule and seedling stages among 57
strains including cultivated rice (Oryza sativa ssp. indica and ssp. japonica) and its wild progenitor (Oryza rufipogon). The significant differentiation of cold tolerance was observed among the taxonomically divided groups. At the germination
stage, both indica and japonica subspecies tended to be more tolerant than O. rufipogon, whereas at the plumule and seedling stages, ssp. japonica tended to be more tolerant than ssp. indica and O. rufipogon. Furthermore, in cold tolerance at the plumule stage, the clinal variation across the latitude of origins was observed within
O. rufipogon and ssp. japonica, suggesting that the current pattern of variation seems to have been shaped by both their phylogenetic histories and on-going
adaptation to the local environments. QTL analysis between O. sativa ssp. japonica (tolerant) and O. rufipogon (susceptible) revealed five putative QTLs for cold tolerance at the plumule and seedling stages but not at the germination
stage. Substitution mapping was also carried out to precisely locate the two major QTLs for cold tolerance at the plumule
stage, which could be used for improvement of tolerance to cold stress in ssp. indica. 相似文献
74.
75.
Koji Adachi Katsuhiro Takehira Tomoaki Soma Masafumi Inoue 《Journal of Wood Science》2010,56(6):502-506
The withdrawal strength of a bolt-nut connector made from wood-based material was evaluated. The thread strength of the wooden
bolt-nut connector was tested to select various parameters of the connector and the type of wood material; the wood materials
tested were hard maple, white oak, ebony, glue-laminated bamboo, and densified Japanese cedar. A plane model of wooden threads
with various thread angles was also evaluated. The results showed that the maximum failure load of the thread increased with
increasing bolt density and connection area, which was calculated from the diameter of the bolt and the thickness of the nut.
The withdrawal resistance after reaching the maximum load underwent a graded decrease because the bolt threads were broken
one by one. In addition, the thread strength depended on the thread angle. In the model with a thread angle of 90°, compressive
deformation in the transverse direction occurred prior to shear deformation along the root of the threads; the model with
this thread angle thus had higher strength than those with other angles. 相似文献
76.
Katsuki Adachi Masaaki Omine Mitsuho Sugimoto Takanori Ishii Hiroshi Niimi Takayuki Suzuki 《Plant Production Science》2016,19(1):125-131
We developed transplantation cultivation method of case-held tuber seedlings (CTS), which was derived from direct planting method of seed tubers, and applied this method to the sweet potato cultivar Beniharuka. A plastic case made of polypropylene was designed for cultivation of CTS. Seed tubers of cultivar Beniharuka in the range of 30–80 g were cut in half. The half-cut tubers were placed inside the plastic cases, and the cases were filled with a commercial soil mix. The case-held tubers were incubated under natural sunlight in a glass house. After 3–4 wk, the CTS were transplanted into a field. Mother tuber (seed tuber) enlargement was suppressed by the plastic confinement of the cases, and daughter tubers were formed above the case as vine-root-originated tubers. In the field experiments in 2012 and 2013, daughter tuber yields were increased 19% and 21% by case-held tuber seedling transplanting (CTST) over conventional vine-planting (VP), the number of daughter tubers per plant in CTST were 36 and 68% higher than in VP, and the mother tuber yields were limited to 2.1 and 4.3% of the total fresh yield of mother and daughter tubers, respectively in 2012 and 2013. Application of CTST method to cultivar Beniharuka enhanced tuber yield, increased the number of daughter tubers per plant, downsized daughter tubers compared to VP, and mother tuber enlargement was suppressed by case-holding. The CTST method is expected to produce more and smaller good in shape tubers of cultivar Beniharuka compared to VP. 相似文献
77.
小菜蛾对4种农药抗药性的研究 总被引:4,自引:0,他引:4
用浸叶法测定了日本关西地区小菜蛾幼虫对乙酰甲胺磷、巴丹、氯氰菊酯和Bt4种不同类型农药的抗药性。试验明确小菜蛾在当地对上述4种参试农药都已产生不同程度抗药性。小菜蛾R系幼虫在240mg/kg氯氰菊酯、200μl/LBt浓度下死亡率分别仅为3.7%、7.2%。这两种农药目前已不能用于防治日本关西地区菜田小菜蛾 相似文献
78.
Kumiko Hayashi Kunihiko Konno Kyoko Adachi Takeshi Nawamura Yoshikazu Shizuri Yuji Okita Ikuo Kimura 《Fisheries Science》2009,75(3):797-803
Suppressive effects of neocarrabiose 4-O-sulfate on the thermal and freeze denaturation of myosin subfragment-1 (S-1) and on myofibrils were investigated. The compound
strongly suppressed the thermal denaturation of S-1. Its suppressive effect was greater than that of sorbitol and similar
to that of maltose. However, it tended to accelerate the denaturation of myofibrils, suggesting a loss of protection by F-actin
upon its addition. The compound suppressed the freeze denaturation of myofibrils and S-1. The effect was similar to that of
sorbitol or maltose, and completely different from that of Na-sulfate. The compound solubilized myofibrils at concentrations
similar to KCl. Therefore, it was concluded that neocarrabiose 4-O-sulfate behaved as an ionic salt in the thermal treatment process, whereas it behaved as a sugar in the frozen storage process. 相似文献
79.
Ryusuke Sudo Ryota Tosaka Shigeho Ijiri Shinji Adachi Hiroaki Suetake Yuzuru Suzuki Noriyuki Horie Satoru Tanaka Jun Aoyama Katsumi Tsukamoto 《Fisheries Science》2011,77(4):575-582
To improve understanding of the mechanism of early ovarian development in eels, the effects of water temperature decrease
on oocyte development, plasma levels of sex steroids [estradiol 17β (E2), testosterone (T), 11-ketotestosterone (11-KT)],
and gonadotropin β-subunit [follicle-stimulating hormone (FSHβ), luteinizing hormone (LHβ)] messenger RNA (mRNA) expression
levels were investigated. A total of 27 female Japanese eels Anguilla japonica were divided into initial, control, and test (water temperature decrease) groups. Starting on 22 September 2009, eels in
the test group were reared in a tank with gradual temperature decrease from 25°C to 15°C over 39 days, while the control group
was maintained at 25°C. The test group accumulated more oil droplets in their oocytes than did the other groups. Levels of
sex steroids, especially 11-KT, were higher in the test group. In contrast, FSHβ and LHβ mRNA expression levels were lower
in the test group. These results suggest that water temperature decrease only induced an early stage of ovarian development
that was partly affected by an 11-KT increase. For further maturation, other environmental factors related to induction of
gonadotropin increase appear to be needed. 相似文献
80.