Expression of natural killer cell stimulatory receptor NKG2D and its ligand MICA in Tibetan patients with gastric cancer at Qinghai plateau

AIM: To investigate the expression of natural killer cell stimulation receptor D(NKG2D) in peripheral blood and the expression of major histocompatibility complex class I chain-related protein A(MICA) on the gastric carcinoma tissue and the neighboring non-cancerous tissue in Tibetan patients with gastric cancer at Qinghai plateau. METHODS: Samples of 33 patients and 20 healthy persons were collected to detect NKG2D in peripheral blood by Flow cytometry analysis. The expressions of MICA in part of the correspondent tissues were examined by RT-PCR and immunohistochemistry.RESULTS: The expression of NKG2D in the plateau of Tibetan patients with gastric cancer group (13.47 ±5.26)% was significantly lower than that in healthy groups (32.62±10.08)%. The mRNA level of MICA in gastric cancer was significantly higher than that in neighboring non-cancerous tissue (P<0.05). The expressions of MICA proteins showed a significant difference between the neighboring non-cancerous tissue (21.21%, 7/33) and the gastric carcinoma tissue (78.79%, 26/33), between the high differentiation (60.00%, 3/5) and the moderate (72.73%, 8/11), low differentiation (88.24%, 15/17). The expressions of MICA were not correlated with tumor size, sex, age, lymph node metastasis of the patients (P>0.05). Spearman rank correlation displayed that the expressions of MICA mRNA were positively correlated with the MICA protein level (r=0.903, P<0.01).CONCLUSION: The immune escape in Tibetan patients with gastric cancer at Qinghai plateau probably relates to the down-regulation of NKG2D and the up-regulation of its ligand MICA. The activity of NK cells and the anti-cancer cellular immunity level are descending in the plateau of Tibetan patients with gastric cancer. The decrease in the receptor NKG2D is a reason for the activity of NK cell descending.     

番茄木虱取食对含Mi-1.2番茄抗氧化酶活性及防御反应相关基因表达的影响

Mi-1.2基因编码CC-NBS-LRR家族抗病蛋白,对线虫及刺吸式昆虫具有广谱抗性。以番茄木虱和携带Mi-1.2基因的番茄品系‘Motelle’作为研究体系,研究番茄木虱取食对番茄叶片抗氧化酶活性及防御反应相关基因表达的影响。结果表明：番茄木虱的取食显著提高了番茄叶片抗氧化酶POD、CAT、APX等的活性,同时诱导Mi-1.2基因表达;番茄木虱的取食上调SA合成有关基因PAL、SA信号途径标志基因PR-1（P4）及与Mi-1.2功能相关基因WRKY70、WRKY72a、WRKY72b的表达。JA/systemin信号途径和伤害有关的PinII、TPI-1、PR-6、PR-7、PR-5等及Prosystemin编码基因Psy的表达未发生变化;JA合成途径相关酶编码基因AOS2、LoxD下调表达,AOC的表达没有变化。SA信号途径可能参与Mi-1.2介导的番茄对于刺吸式昆虫番茄木虱的防御反应。另外,Mi-1.2的RNAi沉默不会改变上述基因的mRNA水平,说明Mi-1.2基因产物对这些基因的表达无调控作用。     

不同氮肥施用量对青花菜氮肥利用率、氮素含量及产量的影响

为了探明不同氮肥施用量对青花菜氮肥利用率、氮素含量和产量的影响,试验设置5个水平,分别为667 m2施0、5、10、20、30 kg,利用相关性分析及回归方程了解氮肥对青花菜的影响。结果表明,氮肥施用量与氮肥利用率间呈显著负相关,与花球中氮素含量呈极显著正相关。青花菜的氮肥效应方程为:Y=-0.829 5X2+33.17 1X+543.55,在其他条件相同的情况下,667 m2氮肥施用量为19.46 kg,青花菜达到最大收益。     

草坪草种(品种)的筛选及利用

以绿色期、质量、抗逆性及建植效率为指标 ,对国内外的优良草坪草进行了引种筛选工作。筛选出了适宜内蒙古气候条件的 9个品种 ,培育出了冷季型草坪草品种—大青山草地早熟禾。同时对优良的地被植物进行了引种驯化     

烷基锡酸催化松香酯化反应的研究

以松香树脂酸的酯化反应为例，研究了烷基锡酸为酸化反应的催化作用，系统的探讨了影响反应的各种因素，研究表明烷其锡酸具有很高的催化活性，可以高效地催化合成系列松香酯，大大缩短了反应时间。     

Allelic variation and composition of HMW-GS in advanced lines derived from d-genome synthetic hexaploid / bread wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.)

The objective of this study was to identify allelic variations at Glu-1 loci of wheat (Triticum aestivum L.) advanced lines derived from hybridization of bread wheat and synthetic hexaploid wheats (2n = 6x = 42; AABBDD). Locally adapted wheat genotypes were crossed with synthetic hexaploid wheats. From the 134 different cross combinations made, 202 F₈ advanced lines were selected and their HMW-GS composition was studied using SDS-PAGE. In total, 24 allelic variants and 68 HMW-GS combinations were observed at Glu-A1, Glu-B1, and Glu-D1 loci. In bread wheat, the Glu-D1 locus is usually characterized by subunits 1Dx2+1Dy12 and 1Dx5+1Dy10 with the latter having a stronger effect on bread-making quality. The subunit 1Dx5+1Dy10 was predominantly observed in these advanced lines. The inferior subunit 1Dx2+1Dy12, predominant in adapted wheat germplasm showed a comparative low frequency in the derived advanced breeding lines. Its successful replacement is due to the other better allelic variants at the Glu-D1 locus inherited in these synthetic hexaploid wheats from Aegilops tauschii (2n = 2x = 14; DD).     

辐照控制菲律宾蛤仔中治病菌及其在贮藏期的生长情况

研究了菲律宾蛤仔经60Coγ射线辐照后,其中的菌落总数、大肠肝菌和副溶血性的D₁₀值及辐照后在贮藏期的变化。结果表明,经60Coγ射线辐照后菲律宾蛤仔中的菌落总数、大肠肝菌、副溶血性的D₁₀值分别为：0.70、0.56和0.20kGy;真空包装的菲律宾蛤仔在0°C~4°C贮藏条件下,3kGy剂量辐照贮藏28d后菌落总数为4.46log(CFU/g),符合水产品卫生要求;而0.4kGy剂量辐照贮藏7d后大厂肝菌、副溶血性弧菌后检测未检出。由此可见,3kGy以上剂量辐照处理菲律宾蛤仔,能有效杀灭其中的微生物,且其贮藏期可达28d,用辐照方法保鲜菲律宾蛤仔具有一定实际意义。     

辐照对冷却猪、鸡肉中细菌ATP发光强度的影响

采用ATP发光法检测辐照对冷却猪、鸡肉ATP发光强度的影响,并对其他试验因素是否对样品ATP发光强度产生影响进行了分析。结果表明:ATP标准品的浓度与发光强度呈显著的正相关,ATP标准品发光强度与辐照剂量表现为显著的负相关;辐照冷却肉的细菌ATP发光强度随辐照剂量的变化趋势呈现为倒S形特征,其中6.0kGy辐照时发光强度最大,4.0、8.0kGy剂量辐照时强度最小;无菌水、灭菌样品未对ATP发光强度造成干扰,而未辐照大肠杆菌浓度与ATP发光强度之间呈显著线性正相关,相关系数达到0.9437。     

抛物线沙丘形态特征与土壤含水量和紧实度的关系研究

以库布齐沙漠南缘典型抛物线沙丘为研究对象,通过对沙丘土壤水分含量与紧实度的测量,分析了沙丘形态特征与土壤水分含量、紧实度的关系。结果表明,沙丘臂间平地水分含量与其周围本底值相似,高于沙丘其它地貌部位。沙丘土壤水分含量高的区域,紧实度较大,抗侵蚀能力较强。沙丘土壤水分含量、紧实度等物理性状特征也影响植被的发育与分布。     

A survey on the faunal diversity of Savar Upazila, Dhaka, Bangladesh

A survey was conducted during January to December 2006 to assess the status of faunal diversity of Savar Upazila, Dhaka, Bangladesh. A total of 30 species of birds, 24 species of winter birds, 7 species of reptiles, 3 species of amphibians, 15 species of mammalians and 32 species of fishes were recorded. Relative abundance of those species were determined. Of the birds, House Sparrow (Passer domesticus) was abundant while Blyth's Kingfisher (Alcedo hercules), Rock Eagle Owl (Bubo bengalensis), Hooded Pitta (Pitta sordida), Black-headed Oriole (Oriolus xanthornus), White-winged Duck (Cairina seululala) and Duck (Anser indicus) were rare. The relative abundance of winter bird could not be assessed because of their migratory habit. Striped keelback (Amphiesma stolata) and Common Smooth Water Snake (Enhydris enhydris) were very common while Black pond turtle (Geoclyms hamiltonii) and Pond tortoise (Melanochelys trijuga) were recorded as endangered. Common Toad (Bufo melanostictus) were abundant but Bull Frog (Rana tigrina) was rare. Asiatic Wild Dog (Cuon alpinus) and House Mouse (Mus musculus) were abundant while Common Otter, Large Indian Civet, Irrawaddy River Dolphin, Indian Hare were rare. Carpu, Silver carp, Tilapia, Nilotica were abundant while, Freshwater Garfish, One stripe spinyeel and Grey Featherback were rare. Landfilling, deforestation, poaching, industrial effluents and current jal were identified as major threats to the faunal diversity of Savar area.