Development of a novel inducible bioluminescent and antibiotic resistance tagging system and its use to investigate the role of antibiotic production by <Emphasis Type="Italic">Pectobacterium carotovorum</Emphasis> ssp. <Emphasis Type="Italic">carotovorum</Emphasis> during potato tuber infection

We report the construction of a novel Tn7 vector for the tagging and enumeration of target bacteria from complex microbial communities. The system utilises a cassette for inducible bioluminescence and tetracycline resistance that integrates at a defined neutral position present in most Gram-negative species. We used this approach to chromosomally tag Pectobacterium such that it could be enumerated in mixed consortia without placing a significant bioburden on the tagged strain. Two Pectobacterium strains, a carbapenem antibiotic producer and an isogenic knock-out strain were tagged using this system. The modified Pectobacterium strains were used to compare the extent to which potato tuber-associated and endophytic bacteria can gain advantage and multiply in planta, utilising the nutrients released by a Pectobacterium infection, when the infecting Pectobacterium is either an antibiotic producer (Car+) or a carbapenem knock-out (Car−) strain. We show that the ability to synthesise carbapenem has a significant effect upon Pectobacterium numbers throughout the course of the infection. Whilst limiting the number of other bacterial species, carbepenem production allows the Pectobacterium to replicate to higher titres in the rotting tuber. We anticipate that the Tn7 tagging vector will be of use to other researchers studying ecological interactions in complex environments.     

Occurrence of atypical myxomatosis in Central Europe: clinical and virological examinations

An outbreak of the atypical form of myxomatosis struck a rabbit farm in Hungary. The animals had previously been vaccinated with a vaccine containing Shope rabbit fibroma virus strain. The disease appeared in winter when the presence of mosquitoes and fleas is not common. The virus was isolated from an eyelid specimen of a naturally infected rabbit. The surviving animals were observed for four weeks, blood samples were collected and, after euthanasia, organ specimens were also examined by morphological methods including pathology and electron microscopy. Serum samples were examined by virus neutralisation for antibodies. Genetic analysis of the isolated virus was carried out by polymerase chain reaction (PCR) and direct sequencing. The primers were designed on the basis of the major envelope gene (Env) of the Lausanne reference strain in the GenBank. The viral proteins were examined by SDS-PAGE. The isolated virus (ref. no.: BP04/2001) was able to infect the susceptible animals directly, by contact. The disease was characterised by respiratory symptoms of the upper tracheal tract, conjunctivitis and high mortality by the 11th-14th day. Aerogenic infection with strain BP04/2001 resulted in 100% morbidity among the susceptible animals. Sequencing of the amplified 400-bp-long DNA revealed 97% homology with the Env gene of the Lausanne strain, which proves that strain BP04/2001 is a variant of the Lausanne strain having been enzootic throughout Europe. The live vaccine strain used in Hungary against myxomatosis, which is also a Lausanne-derived strain, protected the animals. According to the protein analysis a protein of 200 kDa in size is not expressed in strain BP04/2001. This is the first report on atypical myxomatosis in Central Europe. The virus spreads by airborne transmission and may cause severe losses in the rabbit population.     

Der Nährwert der in Ungarn angebauten Reissorten

Zusammenfassung Verfasser untersuchten den Rohproteingehalt, die Eiweißfraktionen, die Aminosäurezusammensetzung der Eiweißfraktionen, den Gehalt an Vitamin der B-Gruppe und die küchentechnischen Eigenschaften von etwa 40 in ungarischen Sortenversuchen eingestellten Reissorten.Die Biologische Wertigkeit der vier im Reiseiweiß vorhandenen Eiweißfraktionen: Orysenin, Albumin, Globulin und Prolamin, wurde nach drei verschiedenen Methoden berechnet.Den Nährstoffverlust der geschälten und geschält polierten Reissorten vergleichend, wurde festgestellt, daß durch Polieren in der Biologischen Wertigkeit der Eiweißstoffe und im Gehalt an Vitaminen der B-Gruppe ein bedeutender Verlust eintritt. Der Verlust an Roheiweiß beträgt etwa 15 bis 29 %. Der größte Verlust, mehr als 75 %, tritt bei der Albuminfraktion ein.Der ungarische Reis ist hinsichtlich des Rohproteingehalts, sowie der küchentechnischen Eigenschaften, ausgezeichnet.

---

The nutritive value of rice species cultivated in Hungary

Summary Authors determined in 40 rice varieties being cultivated or tested in Hungary, the amount of raw protein, protein fractions, amino acid composition of the protein fractions, the amount of vitamins of the B group, and the cooking properties.The nutritive value of the four fractions contained in rice protein, namely oryzenine, albumine, globuline and prolamine, was calculated by three different methods.Comparing husked rice with husked polished rice, it is obvious that polishing causes heavy losses in the nutritive value of proteins and in vitamins of the B group. The losses in raw proteins amounts from 15 to 29 per cent. The greatest loss, exceeding more than 75 per cent, is manifested in the albumin fraction.In view of the high raw protein contents and the advantageous cooking properties, Hungarian rice varieties may be called excellent.

---

La valeur nutritive des espèces de riz cultivées en Hongrie

Résumé Les auteurs ont examiné la teneur en protéines brutes, les fractions protéiques et la teneur en acides aminés des fractions protéiques, ainsi que la teneur en vitamines du groupe B et les propriétés culinaires de 40 espèces de riz cultivées en Hongrie. La valeur nutritive des quatre fractions présentes dans la protéine du riz: Oryzeanine, albumine, globuline et prolamine ont été calculées selon trois méthodes différentes.En comparant le riz décortiqué et le riz décortiqué et poli il est évident que par le polissage une perte significative se manifeste dans la valeur nutritive des substances protéiques ainsi que dans le contenu en vitamines groupe B. La perte en protéine brute varie de 15 jusqu'à 29 %. La plus grande perte environ 75 % s'observe dans la fraction albumine.Le riz hongrois se distingue par grande teneur en protéine et par ses propriétés culinaires avantageuses.

---

---

mit 1 Fig.     

Characterisation of black locust isolates of <Emphasis Type="Italic">Peanut stunt virus</Emphasis> (PSV) from the Pannon ecoregion show the frequent occurrence of the fourth taxonomic PSV subgroup

Biological and molecular characterisation of ten Peanut stunt virus (PSV) isolates from Robinia pseudoacacia was carried out. The host range of these isolates was similar to that of the previously described PSV strains in most cases, but on Pisum sativum and Lens culinaris latent infection was induced. Variability in systemic symptoms was observed only on Nicotiana glutinosa. The partial RNA3 sequences were determined, including the carboxyl terminal region of the movement protein gene, the intergenic region, the entire coat protein gene and the 3′ untranslated region. Nucleotide sequence comparison of the coat protein genes showed 77.6–84.2% identity with most of the known PSV strains and 96.3–98.0% identity with PSV-Rp the typical member of subgroup IV. Phylogenetic analysis indicated the presence of the ancient homologous recombination in all of the examined black locust isolates and all the isolates were members of the fourth PSV subgroup. These results showed that the isolates of the fourth subgroup are widely distributed in black locust in this region.     

Developmentally regulated piRNA clusters implicate MILI in transposon control

Nearly half of the mammalian genome is composed of repeated sequences. In Drosophila, Piwi proteins exert control over transposons. However, mammalian Piwi proteins, MIWI and MILI, partner with Piwi-interacting RNAs (piRNAs) that are depleted of repeat sequences, which raises questions about a role for mammalian Piwi's in transposon control. A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. We also find evidence of an adaptive amplification loop in which MILI catalyzes the formation of piRNA 5' ends. Mili mutants derepress LINE-1 (L1) and intracisternal A particle and lose DNA methylation of L1 elements, demonstrating an evolutionarily conserved role for PIWI proteins in transposon suppression.     

Effect of broth from meat of linseed‐fed cattle on glucose‐stimulated insulin release in healthy male volunteers

Polyunsaturated fatty acid consumption has been shown to improve insulin sensitivity. We studied if administration of broth with beef meat enriched with polyunsaturated fatty acids influenced glucose‐stimulated insulin release in healthy male volunteers. Broth was made either from cattles undergone dietary supplementation with lightly bruised whole linseed in addition to feeding ad libitum on grass silage (test meal) or from those fed grass silage alone (control meal). Oral glucose tolerance tests (OGTT) were performed in patients after a 6‐day period of eating 300 ml broth containing 100 g meat once a day in addition to their otherwise normal mixed nourishment. During OGTT, blood samples were taken for blood glucose level and plasma insulin immunoreactivity before and 15, 30, 60, 90, 120, and 180 min after the glucose load. Glucose‐stimulated maximum increase in plasma insulin immunoreactivity was 42 ± 6.6 and 81 ± 7.4 mU/ml (p < 0.05) after the test and the control meals, respectively. However, both fasting and postload blood glucose levels were the same after either meal period. The results suggest an insulin‐sensitizing effect of food produced from beef cattle maintained on linseed diet in healthy human volunteers.     

Comparison of protein quality and mineral element concentrations in grain of spelt (Triticum spelta L.) and common wheat (Triticum aestivum L.)

The cultivation of Triticum spelta (spelt) has no tradition in Hungary. In recent years the interest towards this old species renewed in many countries. This high‐nutritional cereal, which has a high ash and fibre content, can be used in many health‐oriented grain‐based food products. Therefore, field experiments have been conducted for some years to test the performance of this species under home growing conditions. Here we report the results of analyses for some important quality parameters of grain samples from the 1996/97 season in comparison with those of older and new home‐grown bread wheat cultivars. Three common wheat cultivars and one advanced spelt line were grown on small plots fertilised with an NPK dose necessary to reach the highest yield and quality. Spikes were sampled weekly from the time of 70–77% grain moisture to full ripening. The grains were analysed for ash, N, P and K content and amino acid composition. Concentrations of 16 other macro + micro elements and in the ripe grains, baking quality parameters were also assessed. The grain development of spelt showed a remarkable time‐lag compared to that of the common wheat cultivars. However, the highest thousand‐grain‐masses, ash, N, and P concentrations were measured in this cultivar after milk ripening. The grains of spelt contained the macro‐nutrient Mg and four micro‐nutrients (Zn, Mn, Fe, Cu) in higher concentrations compared to those of the common wheat varieties. The total and essential amino acid concentrations measured in the ripe grains of spelt were also remarkably higher.

Although its wet gluten content (47.5%) was considerably higher than that of the bread wheat cultivars, its breadmaking quality was poor.     

Studying the effect of organic matter recycling combined with mineral n fertilization in long-term field and model pot experiments: Untersuchung der wirkung der rezyklierung der organischen substanz kombiniert mit mineraldüngung in dauerfeldversuchen und modellgefässversuchen

The effect of various combinations of organic and mineral N fertilizers was studied on the organic matter content of the soil, grain yield, dry matter production and N-uptake of maize. In a model experiment, large pots were filled with 55?kg sandy loam soil originating from the plots of the ‘International Long-term Experiments for Investigating the Effect of Organic and Inorganic Fertilisers’ (IOSDV), Keszthely. The field experiment, which was set up in 1983, contained three crop-rotations with maize, winter wheat and winter barley. Treatments: (1) inorganic fertilizers only with increasing N-doses (N); (2) farmyard manuring (FYM) in every third year+N; (3) stalk, straw or green manure+N. Our results clearly proved the advantage of organic matter recycling combined with mineral fertilization over treatment 1. In the fifth and sixth rotations, both types of organic matter recycling resulted in significantly higher C_org contents compared to mineral N fertilization without farmyard manuring or incorporation of crop residues. Grain yields in the field trial were the lowest in treatments without organic matter recycling. In the pot experiment, dry matter production and N accumulation in the grains and vegetative plant parts were also significantly higher in treatments where mineral fertilization was combined with organic matter recycling.