Antibody response of cattle to rinderpest vaccine

Antibody production was studied in cattle infected with rinderpest vaccine virus. Vaccinated cattle produced both IgM and IgG serum antibodies. The IgG antibodies were mainly those of IgG2 subclass. No IgA antibody response was detected in vaccinated animals.     

In situ sprouting and regulation of fresh-seed dormancy in Spanish type groundnut (Arachis hypogaea L.)

Two hundred groundnut (Arachis hypogaea L.) germplasm accessions and 21 cultivars belonging to the Spanish group (ssp. fastigiata var. vulgaris) were screened for pod loss due to sprouting of seed in the field. Some cultivars were also tested for germination of the fresh-seed with or without testa and treated or untreated with ethrel or abscisic acid (ABA). Large variation in pod loss due to in situ sprouting of seed, and fresh-seed dormancy (FSD) was found among the accessions and cultivars. Fresh-seed dormancy index (FSDI) varied from 2% in Chico to 88% in ICGS 44 (the check with high FSD). Cultivars with an FSDI value of less than 10% showed more pod loss in situ than the cultivars with high FSDI. Thus pod loss due to in situ sprouting increased with a decrease in FSDI. Cultivar SB XI did not show any in situ sprouting or pod loss. A direct relationship (r, 0.86^**) existed between fresh-seed germination of accession/cultivar in the laboratory and the percentage of its plants with sprouted seed in the field. Seed of two Spanish cultivars ICGS 11 (dormant) and GG 2 (non-dormant) when tested for germination after treatment with ethrel or ABA at various seed development stages showed different germination response to these chemicals. Seed of GG 2 showed up to 40% germination even at an early stage (S₁) of seed development, whereas the seed of ICGS 11 responded to the ethrel only at maturity (S₄). Regulation of FSD appeared to be more under the control of the testa than the cotyledons. The variation in the degree of in situ sprouting can be used for breeding Spanish cultivars with various desirable levels of FSD.     

Water transmission characteristics of a Vertisol and water use efficiency of rainfed soybean (Glycine max (L.) Merr.) under subsoiling and manuring

In Vertisols of central India erratic rainfall and prevalence of drought during crop growth, low infiltration rates and the consequent ponding of water at the surface during the critical growth stages are suggested as possible reasons responsible for poor yields (<1 t ha⁻¹) of soybean (Glycine max (L.) Merr.). Ameliorative tillage practices particularly deep tillage (subsoiling with chisel plough) can improve the water storage of soil by facilitating infiltration, which may help in minimizing water stress in this type of soil. In a 3-year field experiment (2000–2002) carried out in a Vertisol during wet seasons at Bhopal, Madhya Pradesh, India, we determined infiltration rate, root length and mass densities, water use efficiency and productivity of rainfed soybean under three tillage treatments consisting of conventional tillage (two tillage by sweep cultivator for topsoil tillage) (S₁), conventional tillage + subsoiling in alternate years using chisel plough (S₂), and conventional tillage + subsoiling in every year (S₃) as main plot. The subplot consisted of three nutrient treatments, viz., 0% NPK (N₀), 100% NPK (N₁) and 100% NPK + farmyard manure (FYM) at 4 t ha⁻¹ (N₂). S₃ registered a significantly lower soil penetration resistance by 22%, 28% and 20%, respectively, at the 17.5, 24.5 and 31.5 cm depths over S₁ and the corresponding decrease over S₂ were 17%, 19% and 13%, respectively. Bulk density after 15 days of tillage operation was significantly low in subsurface (15–30 cm depth) in S₃ (1.39 mg m⁻³) followed by S₂ (1.41 mg m⁻³) and S₁ (1.58 mg m⁻³). Root length density (RLD) and root mass density (RMD) of soybean at 0–15 cm soil depth were greater following subsoiling in every year. S₃ recorded significantly greater RLD (1.04 cm cm⁻³) over S₂ (0.92 cm cm⁻³) and S₁ (0.65 cm cm⁻³) at 15–30 cm depth under this study. The basic infiltration rate was greater after subsoiling in every year (5.65 cm h⁻¹) in relation to conventional tillage (1.84 cm h⁻¹). Similar trend was also observed in water storage characteristics (0–90 cm depth) of the soil profile. The faster infiltration rate and water storage of the profile facilitated higher grain yield and enhanced water use efficiency for soybean under subsoiling than conventional tillage. S₃ registered significantly higher water use efficiency (17 kg ha⁻¹ cm⁻¹) over S₂ (16 kg ha⁻¹ cm⁻¹) and S₁ (14 kg ha⁻¹ cm⁻¹). On an average subsoiling recorded 20% higher grain yield of soybean over conventional tillage but the yield did not vary significantly due to S₃ and S₂. Combined application of 100% NPK and 4 t farmyard manure (FYM) ha⁻¹ in N₂ resulted in a larger RLD, RMD, grain yield and water use efficiency than N₁ or the control (N₀). N₂ registered significantly higher yield of soybean (1517 kg ha⁻¹) over purely inorganic (N₁) (1392 kg ha⁻¹) and control (N₀) (898 kg ha⁻¹). The study indicated that in Vertisols, enhanced productivity of soybean can be achieved by subsoiling in alternate years and integrated with the use of 100% NPK (30 kg N, 26 kg P and 25 kg K) and 4 t FYM ha⁻¹.     

Agronomic productivity,nitrogen fertilizer savings and soil organic carbon in conservation agriculture: efficient nitrogen and weed management in maize-wheat system

Conservation agriculture (CA) practice increases agronomic productivity and soil fertility, yet CA stimulate nitrogen (N) immobilization and weed interference during the early periods of implementation. This study focuses on efficient N management by soil testing and optical sensor (GreenSeeker^TM) information; and weed management using brown manuring (Sesbania aculeata co-culture) and herbicide mixtures under CA-based maize (Zea mays L.) – wheat (Triticum aestivum L. emend Fiori & Paol) system in the Indo-Gangetic Plains. Fertilizer N application guided by the optical sensor increased grain yields of maize and wheat up to 20 and 14% (average of two years), respectively, compared to whole N application at sowing. Weed management using brown manuring in maize and herbicide mixtures in wheat increased the grain yields up to 10 and 21%, respectively, over the weedy check. The optical sensor-based N management saved up to 45 and 30 kg N ha^–1 of the optimized N fertilizer rate in maize and wheat, respectively, over whole N application. Fertilizer N management coupled with brown manuring resulted in 5 and 4% higher soil organic carbon accumulation. Implementing efficient N fertilizer and weed management in the early years of CA can improve agronomic yield, fertilizer savings, and soil organic carbon content.     

Development of a semiselective medium for isolating Xanthomonas campestris pv. musacearum from insect vectors, infected plant material and soil

A semiselective medium was developed for isolating Xanthomonas campestris pv. musacearum ( Xcm ) from infected banana plants, soil and insect vectors. The new medium was named cellobiose-cephalexin agar (CCA) and it contained (L⁻¹): 1 g yeast extract, 1 g glucose, 1 g peptone, 1 g NH₄Cl, 1 g MgSO₄·7H₂O, 3 g K₂HPO₄, 1 g beef extract, 10 g cellobiose, 14 g agar, 40 mg cephalexin, 10 mg 5-fluorouracil and 120 mg cycloheximide. The medium was evaluated for selectivity using 21 bacterial isolates and for plating efficiency using Xcm . The bacterial isolates included a soilborne Xanthomonas species and three pathogenic Xanthomonas strains that infect cassava, cabbage and beans. Although the plating efficiency of Xcm on CCA was lower (59%) than on non-selective yeast extract peptone glucose agar (YPGA), its selectivity was significantly higher, averaging 60 and 82%, when isolating from banana fruits and soil, respectively. CCA was also superior when isolating Xcm from insect vectors, with selectivity of 48–75%, compared with 8–17% on YPGA. Xanthomonas campestris pv. phaseoli did not grow on CCA, while X. campestris pv. campestris and X. axonopodis pv. manihotis grew, but their colonies were smaller than those of Xcm . Twenty-nine out of 33 suspected Xcm strains isolated from plants, soil and insects using CCA were pathogenic when inoculated onto banana plants, indicating that CCA can be a reliable tool in isolating Xcm populations. The medium should prove useful in studies on ecology, epidemiology and management of the banana bacterial wilt pathogen that is currently ravaging bananas in East and Central Africa.     

Enzyme activities and microbial biomass in coastal soils of India

Soil salinity is a serious problem for agriculture in coastal regions, wherein salinity is temporal in nature. We studied the effect of salinity, in summer, monsoon and winter seasons, on microbial biomass carbon (MBC) and enzyme activities (EAs) of the salt-affected soils of the coastal region of the Bay of Bengal, Sundarbans, India. The average pH of soils collected from different sites, during different seasons varied from 4.8 to 7.8. The average organic C (OC) and total N (TN) content of the soils ranged between 5.2-14.1 and 0.6-1.4 g kg⁻¹, respectively. The electrical conductivity of the saturation extract (ECe) of soils, averaged over season, varied from 2.2 to 16.3 dSm⁻¹. The ECe of the soils increased five fold during the summer season (13.8 dSm⁻¹) than the monsoon season (2.7 dSm⁻¹). The major cation and anion detected were Na⁺ and Cl⁻, respectively. Seasonality exerted considerable effects on MBC and soil EAs, with the lowest values recorded during the summer season. The activities of β-glucosidase, urease, acid phosphatase and alkaline phosphatase were similar during the winter and monsoon season. The dehydrogenase activity of soils was higher in monsoon than in winter. Average MBC, dehydrogenase, β-glucosidase, urease, acid phosphatase and alkaline phosphatase activities of the saline soils ranged from 125 to 346 mg kg⁻¹ oven dry soil, 6-9.9 mg triphenyl formazan (TPF) kg⁻¹ oven dry soil h⁻¹, 18-53 mg p-nitro phenol (PNP) kg⁻¹ oven dry soil h⁻¹, 38-86 mg urea hydrolyzed kg⁻¹ oven dry soil h⁻¹, 213-584 mg PNP kg⁻¹ oven dry soil h⁻¹ and 176-362 mg PNP g⁻¹ oven dry soil h⁻¹, respectively. The same for the non-saline soils were 274-446 mg kg⁻¹ oven dry soil, 8.8-14.4 mg TPF kg⁻¹ oven dry soil h⁻¹, 41-80 mg PNP kg⁻¹ oven dry soil h⁻¹, 89-134 mg urea hydrolyzed kg⁻¹ oven dry soil h⁻¹, 219-287 mg PNP kg⁻¹ oven dry soil h⁻¹ and 407-417 mg PNP kg⁻¹ oven dry soil h⁻¹, respectively. About 48%, 82%, 48%, 63%, 40% and 48% variation in MBC, dehydrogenase activity, β-glucosidase activity, urease activity, acid phosphatase activity and alkaline phosphatase activity, respectively, could be explained by the variation in ECe of saline soils. Suppression of EAs of the coastal soils during summer due to salinity rise is of immense agronomic significance and needs suitable interventions for sustainable crop production.