Angiotensin Converting Enzyme in Bovine Ovarian Follicular Fluid and its Relationship with Oestradiol and Progesterone

The purpose of the present study was to identify angiotensin converting enzyme (ACE) in bovine ovarian follicular fluid and to relate the ACE activity to the phase of the oestrous cycle, pregnancy, and the follicular fluid concentrations of oestradiol and progesterone. The ACE activity was similar to that found in bovine serum and was completely inhibited by the specific ACE inhibitor captopril. The 50% inhibitory concentration (IC₅₀) was 1.4 × 10^?8 mol/l (range 0.8 × 10^?8 to 5.0 × 10^?8 mol/l; n=6), which is similar to that found in bovine and human serum. The ACE activity did not differ in the pre‐ovulatory and luteal phase, pregnancy or cystic follicles. It correlated with the follicular fluid concentration of progesterone in cycling cows (ρ=0.476; p < 0.005; n=36), but did not correlate with the diameter of the follicles, the follicular fluid concentration of oestradiol or the ratio between the oestradiol and progesterone concentrations. The demonstration of ACE in bovine ovarian follicular fluid provides further evidence for the presence of a local renin–angiotensin system in the bovine ovary.     

Evaluation of the soil penetration resistance along a transect to determine the loosening depth

The objective of this study was to evaluate the distribution of soil strength (measured as cone index, CI) along a 600 m transect and to determine the soil loosening depth necessary to eliminate zones with soil strengths exceeding a threshold value down to a depth of 0.6 m. The transect was located at a site in a glacial drift area which was characterised by sandy deposits overlying boulder clay. A tractor-mounted multi-penetrometer array consisting of four hydraulically driven single vertical penetrometers was used to determine CI at 1-m sampling intervals as a measure of penetration resistance. The spatial fluctuation of the CI readings in general and that of repeatedly averaged readings along the transect was examined. Furthermore, the relationships between the penetration resistance of several soil layers and the relationships between the CI of single penetrometers were identified. Averaged CI values over 5-m intervals were used to determine the depth of soil loosening required. By using various data sub-sets based on the averaged data of the four array mounted penetrometers and simulating several different sampling intervals, treatment intervals and threshold values of soil strength, a sampling interval of about 10 m proved to be sufficiently accurate to determine the loosening depth required.Special Issue: Papers from the 5th European Conference on Precision Agriculture (5ECPA), Uppasala, Sweden, 2005.