Plasmids in Pseudomonas syringae pv. pisi carry genes for pathogenicity

All strains tested which are pathogenic to peas and which react with antiserum to Pseudomonas syringae pv. pisi contain two to four plasmids; non-pathogens contain none. Two plasmids from a pathogenic strain were transferred individually to a non-pathogenic recipient strain of Pseudomonas syringae: both plasmids converted the recipient to a pathogen on peas and from hypersensitivity negative to hypersensitivity positive on tobacco. Neither plasmid encoded homoserine catabolism.     

QUANTITATIVE HEPATOBILIARY SCINTIGRAPHY USING 99mTC-DISIDA IN THE DOG

Quantitative hepatobiliary scintigraphy using ^99mTc-2,6 diisopropylphenylcarbamoylmethyl iminodiacetic acid (DISIDA) was performed in normal dogs. ^99mTc-DISIDA is excreted by the hepatocyte into the biliary tract. Hepatic blood flow from arterial and portal venous sources was measured as the hepatic perfusion index. The hepatic clearance of the radiopharmaceutical was used to assess hepatocyte function and parenchymal biliary transport. Biliary ejection was evaluated using synthetic cholecystokinin infusion. The procedure should be useful in evaluating dogs with hepatic and biliary disease.