全文获取类型
收费全文 | 40045篇 |
免费 | 1502篇 |
国内免费 | 2573篇 |
专业分类
林业 | 5798篇 |
农学 | 3643篇 |
基础科学 | 1749篇 |
5740篇 | |
综合类 | 10413篇 |
农作物 | 3777篇 |
水产渔业 | 3034篇 |
畜牧兽医 | 4459篇 |
园艺 | 2497篇 |
植物保护 | 3010篇 |
出版年
2024年 | 114篇 |
2023年 | 371篇 |
2022年 | 843篇 |
2021年 | 957篇 |
2020年 | 912篇 |
2019年 | 908篇 |
2018年 | 3349篇 |
2017年 | 3725篇 |
2016年 | 2028篇 |
2015年 | 1258篇 |
2014年 | 1239篇 |
2013年 | 1453篇 |
2012年 | 2700篇 |
2011年 | 4124篇 |
2010年 | 3927篇 |
2009年 | 2968篇 |
2008年 | 2927篇 |
2007年 | 3114篇 |
2006年 | 1372篇 |
2005年 | 1126篇 |
2004年 | 794篇 |
2003年 | 700篇 |
2002年 | 714篇 |
2001年 | 590篇 |
2000年 | 520篇 |
1999年 | 280篇 |
1998年 | 184篇 |
1997年 | 157篇 |
1996年 | 90篇 |
1995年 | 118篇 |
1994年 | 127篇 |
1993年 | 100篇 |
1992年 | 84篇 |
1991年 | 67篇 |
1990年 | 41篇 |
1989年 | 35篇 |
1988年 | 39篇 |
1987年 | 18篇 |
1986年 | 4篇 |
1985年 | 7篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1981年 | 5篇 |
1980年 | 5篇 |
1979年 | 4篇 |
1977年 | 4篇 |
1968年 | 4篇 |
1962年 | 2篇 |
1956年 | 2篇 |
1932年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
61.
62.
63.
64.
65.
AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P<0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P<0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P<0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P<0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P<0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation. 相似文献
66.
AIM:To study the effect of environment of liver regeneration on the proliferation of rat fetal hepatocytes after intrasplenical transplantation. METHODS:Fetal hepatocytes isolated from 3-week SD rat fetuses bred were transplanted into the spleens of liver regeneration model rats with 70% partial hepatectomy. The cell cycle of the hepatocytes in the remnants liver was analyzed by flow cytometer and the density dimensions of the donor fetal hepatocytes in spleen were measured by image analysis system 7 and 30 days post-transplantation, respectively. RESULTS:Compared with the control group, the proportions of S and G2 /M cells in the remnants liver were obviously decreased (P<0.05), but the density dimensions of the donor fetal hepatocytes in spleen increased significantly (P<0.05) in rats with hepatectomy 7 days post-transplantation. CONCLUSION:The environment of liver regeneration is propitious to the proliferation of fetal hepatocytes after transplantation into spleen. 相似文献
67.
LIN Jun SUN Jie ZHOU Yan HUANG Xing XIONG Ping WANG Ya-ping DENG Chang-sheng 《园艺学报》2002,18(9):1112-114
AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal neoplasm in Hubei Han Chinese. METHODS: HLA-DRB1 gene polymorphism in 42 patients with esophageal neoplasm and 136 normal control subjects was studied by PCR and sequence. RESULTS: Allele frequency of HLA-DRB1 *0901 allele was significantly higher in esophageal cancer patients than those in normal controls(0.2500 vs 0.1397, P =0.028; the odds ratiO2.053; etiologic fraction 0.1282).There were no association between the rested HLA-DRB1 alleles with patients. CONCLUSION: Individuals carrying HLA-DRB1 *0901 may be susceptible to esophagealo carcinoma, its nucleotide sepuence approachs to the corresponded allele sequence(exoN2)published in GenBank. 相似文献
68.
69.
Eiji Tanaka Chihiro Tanaka Atsushi Ishihara Yasumasa Kuwahara Mitsuya Tsuda 《Journal of General Plant Pathology》2003,69(1):1-6
Aciculosporium take (Ascomycota; Clavicipitaceae) is a causal agent of witches' broom of
bamboo plants. The symptoms of this disease are believed to be induced by plant
hormones, particularly auxins. Indole-3-acetic acid (IAA) was identified in
cultures of this fungus in an l-tryptophan-supplemented liquid medium.
IAA production was confirmed on 30 isolates of A. take from various
hosts and locations at levels up to 1 mg/l. The biosynthetic pathway of
IAA in A. take culture was examined by analyzing intermediate products
and by feeding experiments. The results showed that the indole-3-pyruvic acid
pathway (l-tryptophan → indole-3-pyruvic acid → indole
acetaldehyde → IAA) was the dominant pathway in A. take.
Received: June 3, 2002 / Accepted: July 25, 2002 相似文献
70.
Hiromitsu Furuya Tsutomu Matsumoto Shin-ichi Fuji Hideki Naito 《Journal of General Plant Pathology》2003,69(2):115-119
Rice seedling growth, estimated by plant height and root development and discoloration, was better in pasteurized soil than
in unpasteurized soil obtained from a flooded rice field. Rice seedlings also grew better in sterilized soil modified by adding
roots harvested from the pasteurized soil than in soil modified by adding roots harvested from the unpasteurized soil. The
results demonstrate that seedling growth in the rice field soil was inhibited by soil microorganisms, even though no typical
symptoms such as seedling blight or damping-off appeared. Pythium aristosporum is suggested to be involved in the inhibition. Thus, it appears that inconspicuous restraint of rice seedling growth could
occur in soils of rice paddy fields.
Received: May 20, 2002 / Accepted: October 16, 2002
Acknowledgments The authors thank Dr. T. Ichitani, former professor at Osaka Prefectural University, for providing an isolate of Pythium aristosporum for comparison, and Mr. Mitsuaki Sato of Akita Prefectural College of Agriculture for technical assistance. 相似文献