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461.
462.
Summary High mortality rates have often been observed when artificial feeding techniques are used for colony maintenance ofOrnithodoros moubata. Such fatalities can be readily controlled by addition of antibiotics to the feeding medium without apparent toxicity to
ticks. Comparative results show that for maintaining colonies ofO. moubata artificial feeding techniques can be as efficient as direct feeding on swine—the primary hosts of these argasids.
Resumen Se ha observado gran mortalidad deOrnithodoros moubata, en colonias alimentadas artificialmente. Esta puede controlarse fácilmente, a?adiendo antibióticos al medio artificial, sin producir toxicidad a las garrapatas. Los resultados comparativos demuestran, que la alimentación artificial de colonias deOrnithodoros moubata es más eficiente, que la alimentación directa de las garrapatas en cerdos, huéspedes naturales de estos argásidos.
Résumé L’alimentation artificielle utilisée pour la maintenance d’une colonie d’Ornithodoros moubata a entra?né de forts pourcentages de mortalité. Ces pertes peuvent être contr?lées par l’addition d’antibiotique au liquide nourricier, sans toxicité apparente pour les tiques. Des résultats comparatifs montrent que pour la maintenance de colonies d’O. moubata, les techniques d’alimentation artificielle sont aussi efficaces que l’alimentation sur porc, h?te primaire de ces argasidés.相似文献
463.
464.
Depletion of taurine and glutamate from damaged photoreceptors in the retinas of dogs with primary glaucoma 总被引:1,自引:0,他引:1
Madl JE McIlnay TR Powell CC Gionfriddo JR 《American journal of veterinary research》2005,66(5):791-799
OBJECTIVE: To determine whether taurine and glutamate contents are reduced in damaged photoreceptors in dogs with primary glaucoma (PG) in a manner consistent with an ischemia-like release of both of these amino acids from damaged cells. SAMPLE POPULATION: Retinas from 6 dogs with PG and 3 control dogs. PROCEDURE: Serial, semithin sections of each canine retina were stained with toluidine blue to identify damaged photoreceptors or via immunogold techniques to quantify taurine and glutamate content in retinal cells. RESULTS: Regions with a thin outer nuclear layer and pathologic nuclear changes in photoreceptors were evident in retinas of dogs with PG. The density of immunostaining for taurine in damaged photoreceptors was significantly reduced to (mean +/- SEM) 37.5 +/- 2.6% of the density in adjacent undamaged photoreceptors. Photoreceptors with decreased taurine immunostaining also had decreased glutamate immunostaining, consistent with ischemia-like release of both of these amino acids from damaged cells. Immunostaining for glutamate, but not taurine, was increased in presumptive radial glial cells (i.e., Miller cells) in damaged regions, consistent with an ischemia-induced redistribution of amino acids in dogs with PG. CONCLUSIONS AND CLINICAL RELEVANCE: Retinal damage in dogs with PG includes ischemia-like losses of taurine and glutamate from photoreceptors and accumulation of glutamate, but not taurine, in nearby Müller cells. These changes are consistent with glutamate release and depletion of intracellular taurine in damaged regions, perhaps contributing to progressive damage in these areas. 相似文献
465.
Lekcharoensuk C Osborne CA Lulich JP Albasan H Ulrich LK Koehler LA Carpenter KA Swanson LL Pederson LA 《Journal of the American Animal Hospital Association》2005,41(1):39-46
Medical records from cats diagnosed with uroliths at nine United States veterinary teaching hospitals from 1980 to 1999, and records of cats with uroliths submitted for analyses to the Minnesota Urolith Center from 1981 to 2000, were evaluated. A 10-fold increase in frequency of upper tract uroliths occurred in cats during the 20-year interval at the nine veterinary teaching hospitals. Calcium oxalate emerged as the predominant mineral type in upper tract uroliths, having increased more than 50-fold during the study period. These results emphasize the need for increased awareness of the occurrence of upper urinary tract uroliths in cats. 相似文献
466.
McIlnay TR Gionfriddo JR Dubielzig RR Powell CC Madl JE 《American journal of veterinary research》2004,65(6):776-786
OBJECTIVE: To determine whether retinal damage in dogs with primary glaucoma (PG) is consistent with ischemia-induced glutamate toxicosis. SAMPLE POPULATION: Retinal tissue sections from 25 dogs with PG and 12 normotensive control dogs. PROCEDURE: Retinal sections from control and glaucomatous dogs were stained for morphometric and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) analyses to determine whether retinal damage was consistent with glutamate toxicosis. Immunohistochemical analysis was performed to detect ischemia-like loss of glutamate from neurons in damaged areas. RESULTS: In severely damaged glaucomatous retinas, all neurosensory layers had focal regions that were thin or disrupted. There was less thinning of the outer nuclear layer (ONL) and inner nuclear layer (INL) in moderately damaged retinas than in severely damaged retinas. Acute signs of damage in the INL included cells with dark, condensed chromatin and lightly stained cytoplasm interspersed with a few TUNEL-positive cells, which was consistent with glutamate toxicosis. Glutamate immunoreactivity was reduced in thin areas and in damaged cells of the INL and ONL, which was consistent with glutamate release in damaged areas. Glutamate immunoreactivity was increased in putative Müller cells in damaged areas, which also was consistent with glutamate release. CONCLUSIONS AND CLINICAL RELEVANCE: Retinal damage in dogs with PG differs in intensity in focal areas. Damage in affected regions resembles damage induced by glutamate. Glutamate is lost from damaged neurons and accumulates in Müller cells, which is consistent with increased glutamate release contributing to the damage. Glutamate antagonists may protect INL cells in dogs with glaucoma. 相似文献
467.
A young female Akita presented for ocular and nasal lesions. Examination showed bilateral 360 degree limbal masses that extended onto the peripheral cornea. In addition, intranasal granulomatous masses were observed by rhinoscopy. Histopathologic examination with immunohistochemical staining showed that these masses were almost identical and were classified as an idiopathic, T-cell rich, granulomatous inflammation. The dog responded well to anti-inflammatory medication and the disease remains in remission 2 years after the initial presentation. 相似文献
468.
469.
Clinical evaluation of dietary modification for treatment of spontaneous chronic renal failure in dogs 总被引:2,自引:0,他引:2
Jacob F Polzin DJ Osborne CA Allen TA Kirk CA Neaton JD Lekcharoensuk C Swanson LL 《Journal of the American Veterinary Medical Association》2002,220(8):1163-1170
OBJECTIVE: To determine whether a diet used for dogs with renal failure (renal food [RF]) was superior to an adult maintenance food (MF) in minimizing uremic crises and mortality rate in dogs with spontaneous chronic renal failure. DESIGN: Double-masked, randomized, controlled clinical trial. ANIMALS: 38 dogs with spontaneous chronic renal failure. PROCEDURE: Dogs were randomly assigned to a group fed adult MF or a group fed RF and evaluated for up to 24 months. The 2 groups were of similar clinical, biochemical, and hematologic status. The effects of diets on uremic crises and mortality rate were compared. Changes in renal function were evaluated by use of serial evaluation of serum creatinine concentrations and reciprocal of serum creatinine concentrations. RESULTS: Compared with the MF, the RF had a beneficial effect regarding uremic crises and mortality rate in dogs with mild and moderate renal failure. Dogs fed the RF had a slower decline in renal function, compared with dogs fed the MF. CONCLUSIONS AND CLINICAL RELEVANCE: Dietary modifications are beneficial in minimizing extrarenal manifestations of uremia and mortality rate in dogs with mild and moderate spontaneous chronic renal failure. Results are consistent with the hypothesis that delay in development of uremic crises and associated mortality rate in dogs fed RF was associated, at least in part, with reduction in rate of progression of renal failure. 相似文献
470.