A Distributed Lag Analysis of the Relationship Between Gibberella zeae Inoculum Density on Wheat Spikes and Weather Variables

ABSTRACT In an effort to characterize the association between weather variables and inoculum of Gibberella zeae in wheat canopies, spikes were sampled and assayed for pathogen propagules from plots established in Indiana, North Dakota, Ohio, Pennsylvania, South Dakota, and Manitoba between 1999 and 2005. Inoculum abundance was quantified as the daily number of colony forming units per spike (CFU/spike). A total of 49 individual weather variables for 24-h periods were generated from measurements of ambient weather data. Polynomial distributed lag regression analysis, followed by linear mixed model analysis, was used to (i) identify weather variables significantly related to log-transformed CFU/spike (the response variable; Y), (ii) determine the time window (i.e., lag length) over which each weather variable affected Y, (iii) determine the form of the relationship between each weather variable and Y (defined in terms of the polynomial degree for the relationship between the parameter weights for the weather variables and the time lag involved), and (iv) account for location-specific effects and random effects of years within locations on the response variable. Both location and year within location affected the magnitude of Y, but there was no consistent trend in Y over time. Y on each day was significantly and simultaneously related to weather variables on the day of sampling and on the 8 days prior to sampling (giving a 9-day time window). The structural relationship corresponded to polynomial degrees of 0, 1, or 2, generally showing a smooth change in the parameter weights and time lag. Moisture- (e.g., relative humidity-) related variables had the strongest relationship with Y, but air temperature- and rainfall-related variables also significantly affected Y. The overall marginal effect of each weather variable on Y was positive. Thus, local weather conditions can be utilized to improve estimates of spore density on wheat spikes around the time of flowering.     

Sebaceous adenitis in a 7-year-old Arabian gelding

A 7-year-old Arabian gelding was presented with a 9-month history of progressive patches of nonpruritic scaling, crusting, alopecia, and leukoderma of the periocular areas and muzzle, becoming generalized over time. Sebaceous adenitis was diagnosed on histopathologic examination. Lesions resolved without treatment, coinciding with regression of a sarcoid on the neck.     

Clinical evaluation of dietary modification for treatment of spontaneous chronic kidney disease in cats

Objective-To determine whether a renal diet modified in protein, phosphorus, sodium, and lipid content was superior to an adult maintenance diet in minimizing uremic episodes and mortality rate in cats with stage 2 or 3 chronic kidney disease (CKD). Design-Double-masked, randomized, controlled clinical trial. Animals-45 client-owned cats with spontaneous stage 2 or 3 CKD. Procedures-Cats were randomly assigned to an adult maintenance diet (n = 23 cats) or a renal diet (22) and evaluated trimonthly for up to 24 months. Efficacy of the renal diet, compared with the maintenance diet, in minimizing uremia, renal-related deaths, and all causes of death was evaluated. Results-Serum urea nitrogen concentrations were significantly lower and blood bicarbonate concentrations were significantly higher in the renal diet group at baseline and during the 12- and 24-month intervals. Significant differences were not detected in body weight; Hct; urine protein-to-creatinine ratio; and serum creatinine, potassium, calcium, and parathyroid hormone concentrations. A significantly greater percentage of cats fed the maintenance diet had uremic episodes (26%), compared with cats fed the renal diet (0%). A significant reduction in renal-related deaths but not all causes of death was detected in cats fed the renal diet. Conclusions and Clinical Relevance-The renal diet evaluated in this study was superior to an adult maintenance diet in minimizing uremic episodes and renalrelated deaths in cats with spontaneous stage 2 or 3 CKD.     

Primary closure of the corneas of two Great Horned owls after resection of nonhealing ulcers

Two Great Horned owls were presented to the Colorado State University Veterinary Teaching Hospital for evaluation of corneal lesions. Each bird had a corneal ulcer and bullous keratopathy. Following unsuccessful attempts at medical and surgical management, the corneal defects in each bird were treated with penetrating keratoplasties and conjunctival pedicle grafts. In each bird the cornea healed well and regained approximately its normal shape. Both birds fully recovered and have been released into the wild.     

Evaluation of retinal images for identifying individual dogs

OBJECTIVE: To F whether vessels in the ocular fundus changed over the lifetime of Beagles and whether any changes were substantial enough to likely preclude positive identification of individual dogs by use of their retinal vascular patterns. ANIMALS: 18 Beagles. PROCEDURES: Fundic photographs of both eyes of 18 Beagles taken at 1 or 3, 5, and 7 or 9 years of age were digitalized. Photographs were analyzed by use of 2 software programs. One was used to determine vessel numbers and widths and the other to determine the locations of the 3 largest vessels. Measurements were compared over time periods in the life of each dog. Only observations made at baseline (1 or 3 years of age) and again at 5 and 9 years of age were included in the statistical analysis, as these points were common to all dogs. RESULTS: No significant changes in numbers or locations of the blood vessels were detected over time. Widths of the vessels decreased significantly as the dogs aged. CONCLUSIONS AND CLINICAL RELEVANCE: The ocular fundus of Beagles changed over each dog's lifetime in that the retinal blood vessels became smaller but did not change in number or location. Results suggest that digitalized retinal images can likely be used to identify dogs over their lifetimes.     

Results of Schirmer tear test in clinically normal llamas (Lama glama)

Purpose To determine the normal reference range for Schirmer tear test (STT) values in clinically normal llamas (Lama glama) Animals Nine captive llamas (Lama glama) (seven females and two males) were used in this study. Procedure Complete ophthalmic examinations were performed without chemical restraint. STT I values were evaluated in both eyes of all llamas using a commercial STT strip of a single lot number (Schirmer‐Tränentest^®, Germany). STT II value was also measured in both eyes of seven female llamas. Results No statistically significant differences among ages or between right and left eyes were found for any of the results. The mean ± SD STT I of 18 eyes of nine llamas was 17.3 ± 1.1 mm/min (Range 15–19 mm/min). The mean ± SD STT II of 14 eyes of seven llamas was 15.4 ± 1.7 mm/min (Range 12.5–17.5 mm/min). A paired samples t‐test demonstrated that there was a significant difference between the STT I and II values (P = 0.001). Conclusion This study provides novel data for normal reference ranges of STT I and II values in healthy llamas. Results of this study may assist veterinarians in the diagnosis of ocular surface disease and syndromes affecting the tear film in these species.     

Peptide derivatives of dermaseptin S4 in fresh bovine semen for bacterial contamination control: Physicochemical and structural characterization,antibacterial potency,and effects on red blood and sperm cells

The objectives of this study were to examine the physicochemical and structural properties of peptide derivatives of dermaseptin S4, investigate their detrimental effects on red blood and sperm cells and ascertain their antibacterial potency to control bacterial contaminants in fresh bovine semen. The dermaseptin S4 peptide derivatives used in this study were K4S4, S4(5–28), S4(5–28)a, K20S4(5–28), K4S4(1–16)a, K4S4(1–15)a and K4S4(1–15). Peptides K4S4, S4(5–28)a, K20S4(5–28), K4S4(1–15)a and K4S4(1–16)a, with a higher positive charge, were the most potent against the bacterial strains tested, with the lowest minimum inhibitory concentration (MIC), whereas S4(5–28) and K4S4(1–15), with a lower positive charge, showed the highest MIC (p < .01). Haemolysis percentage depended on peptide concentration (p < .01). The K4S4 was the most powerful haemolytic peptide, showing the highest haemolysis percentage at all peptide concentrations (p < .01). In contrast, S4(5–28), S4(5–28)a, K20S4(5–28) and K4S4(1–15) were not able to produce 50% cell lysis up to 100 µM (p < .01). All peptides reduced sperm motility in a dose-dependent manner when used in concentrations from 16 to 64 μM (p < .01). The highest reduction was seen due to K4S4 activity, and the lowest reductions of sperm motility were observed due to K4S4(1–16)a and K4S4(1–15)a activity (p < .01). Hence, we can conclude that K4S4(1–16)a and K4S4(1–15)a at a concentration of approximately 15 µM are the most promising peptides as antibacterial agents in fresh bovine semen, because at this concentration, they showed the most potent antibacterial activity against evaluated strains without significant effects on haemolysis or a reduction in sperm motility.